Research on Q-markers of Eupatorium lindleyanum based on analytic hierarchy process-entropy weight method and network pharmacology / 中国中药杂志

The potential quality markers( Q-markers) of Eupatorium lindleyanum were studied with analytic hierarchy process(AHP)-entropy weight method(EWM) and network pharmacological method. Based on the concept of Q-markers of traditional Chinese medicine, AHP-EWM was employed to quantitatively identify the Q-markers of E. lindleyanum. AHP method was applied to the weight analysis of the validity, testability, and specificity of the first-level indexes, and EWM method was used to analyze the secondlevel indexes supported by literature and experimental data. At the same time, based on the theory and method of network pharmacology, the component-target-disease-efficacy network of E. lindleyanum was built, and the components most closely related to the efficacy of resolving phlegm and relieving cough and asthma were screened out. Through the integrated analysis of the results obtained with AHP-EWM and network pharmacological method, 13 compounds including rutin, quercetin, nepetin, cirsiliol, luteolin, hyperoside,isoquercitrin, kaempferol, caffeic acid, eupalinolide K, eupalinolide A, eupalinolide B, and eupalinolide C were comprehensively identified as the potential Q-markers of E. lindleyanum. The results provide a basis for the quality control of E. lindleyanum.

Analysis of chemical constituents in Eupatorium lindleyanum by UPLC-Q-TOF/MS / 中草药

Objective: To analyze and identify the chemical constituents from Lindley eupatorium by using UPLC-Q-TOF/MS. Methods: The separation was performed on Waters Acquity UPLC BEH C18 (100 mm × 2.1 mm, 1.7 μm) column with gradient elution of 0.1% formic acid (A)-acetonitrile (B), the flow rate was 0.2 mL/min. The column temperature was set at 35 ℃. The MS analysis was based on information associated mode (IDA), and positive and negative ions were collected respectively. Results: A total of 26 compounds in L. eupatorium were identified by PeakView, combined with the mass spectrometry data of each chromatographic peak in the database, and the cleavage law of secondary fragment of each peak of which11 compounds were first reported for L. eupatorium. The main chemical constituents included flavonoids, nucleosides, alkaloids, phenylpropanoid, sesquiterpenoids, coumarins, polyols, etc. Conclusion: The method is accurate, reliable and effficient, which is suitable for rapid identification of ingredients in L. eupatorium, which provides a reference for clarify its efficacy and material basis.

Study on the Antihypertensive Effects of Water Decoction of Eupatorium lindleyanum on Spontaneously Hy-pertensive Rats and Corresponding Mechanism / 中国药房

OBJECTIVE:To study the antihypertensive effects of water decoction of Eupatorium lindleyanum on spontaneously hypertensive rats (SHR) and corresponding mechanism. METHODS:50 SHR were randomly divided into model group,captopril tablets group(positive drug,10 mg/g)and the groups of high,medium and low-dose(65.45,32.73,16.36 g/kg)water decoction of E. lindleyanum,with 10 rats in each group. The rats in the model group were given normal saline ig,while those in other groups were given corresponding drugs ig,once a day,for 7 consecutive weeks. The systolic blood pressure (SBP) and diastolic blood pressure(DBP)of the caudal arteries of the rats were measured every week,and the dynamic changes in the blood pressure of the rats as a result of drug intervention were recorded. The rats were sacrificed 7 weeks later,and the levels of endothelin(ET), angiotensin (AngⅡ) and nitric oxide (NO) in serum were determined. RESULTS:After 7 weeks of drug use,compared to the model group,the rats in the drug administration groups had significantly lower SBP and DBP of the caudal artery(P<0.05),show-ing obvious dose-effect and time-effect relationships in the antihypertensive effect of water decoction of E. lindleyanum,and had ob-viously lower levels of NO and AngⅡ and markedly higher level of ET in serum,demonstrating a dose-effect relationship. CON-CLUSIONS:The water decoction of E. lindleyanum has an obvious antihypertensive effect by a mechanism which may be related to decrease in the levels of NO and AngⅡin serum and increase in the level of ET therein.