Tratamiento de punta nasal mestiza en rinoplastía secundaria / Treatment of mestize tip nose in secundary rhinoplasty

ANTECEDENTES: la nariz mestiza constituye un reto en la adecuada trasformación estética y funcional de la rinoplastia, más aún cuando esta se realiza en una segunda intervención y los tejidos nasales ya se encuentran alterados, no pudiendo hacer una adecuada reconstrucción de las estructuras anatómicas sin el uso de injertos cartilaginosos de otras áreas del cuerpo. OBJETIVO: el propósito de este trabajo es de evaluar los resultados obtenidos de una reconstrucción de la punta nasal, realizada en pacientes que ya fueron sometidos a una rinoplastia sea esta secundaria o cuaternaria. METODOLOGIA: estudio retrospectivo, de casos de pacientes que se sometieron a una nueva intervención para mejorar aspectos estéticos y funcionales nasales, que ya fueron operados en más de 1 oportunidad. HALLAZGOS: se tiene un universo de 10 pacientes en los que se realizó una rinoplastia secundaria o cuaternaria donde se utilizó injerto costal para conformar el esqueleto cartilaginoso y el uso de un injerto de extensión septal, todo esto para mejorar la estética y funcionalidad nasal.

Espaço Univer-cidade e PELC: a experiência do grupo GEPLEC/UFPNA gestão do programa de esporte e lazer na cidade de curitiba / Space Universe-city and PELC: the experience of the group GEPLEC/UFPR managemente sport and leisure program in the city of curitiba

O presente texto visa relatar os caminhos didático-metodológicos norteadores da implantação do PELC/UFPR, no período de 2008 a 2009, desenvolvido pelo Grupo de Estudos e Pesquisas em Lazer, Espaço e Cidade da UFPR (GEPLEC), localizado no CEPELS/DEF/UFPR. Esta ação se consolidou de forma articulada entre ensino-pesquisaextensão, com intervenções práticas no âmbito do lazer para a comunidade e formação continuada para professores e educadores. Para tanto, os conteúdos como jogo, esporte, dança, lutas, teatro, música, capoeira, práticas corporais na natureza, entre outras ações, foram desenvolvidos, ampliados e transformados. Tal ação partiu do princípio que as experiências no tempo-espaço do lazer podem se constituir como uma prática sociocultural, pedagógica e política.

This paper aims at reporting the ways teaching and methodological guide the deployment of PELC/UFPR in the period 2008 to 2009, developed by the Group of Leisure Studies and Research, Space and the City UFPR (GEPLEC), located in CEPELS/DEF/UFPR. This action was consolidated in coordination between education-researchextension, with practical interventions in the context of recreation for the community and continuing education for teachers and educators. For both, the contents such as game, sports, dance, fights, drama, music, capoeira, bodily practices in nature, among other actions, have been developed, expanded and transformed. Such action was assumed that the experiments in space-time leisure can be constituted as a sociocultural practice, pedagogy and politics.

Improving the affinity of an anti-TNF? scFv by error prone PCR and staggered extention process(StEP) / 中国免疫学杂志

Objective:To improve the affinity of an anti TNF? scFv.Methods:Starting from an anti TNF? scFv gene a mutant phage antibody library was generated by error prone PCR.Affinity improved clones were selected and subjected to staggered extension process to shuffle the mutated sites.Mutants with further improved affinity were selected by bio panning.Affinity was judged by dot blot ELISA and thiocyanate elusion ELISA.Results:Seven affinity improved mutants were obtained from library constructed by error prone PCR.By StEP mediated shuffling of these 7 clones and via bio panning,mutants with further improved affinity were obtained.Conclusion:Combination of error prone PCR and StEP could be used to improve the affinity of antibodies. [

Construction of chimeric CblN/Grb2 and its expression in prokaryon / 解放军医学杂志

Objective To construct the pGEX-4T-2-CblN/Grb2, express and purify the corresponding chimeric protein; To investigate whether the chimeric CblN/Grb2 possesses ubiquitin ligase activity. Methods Total RNA of SK-BR-3 cells were isolated and reversely transcribed into cDNA, which were used as templates to amplify Grb2 SH2 by PCR. The gene fragment encoding the N-terminal part of Cbl protein (named as CblN) was amplified by PCR using pEFHACbl plasmid encoding human Cbl as templates. BamH I and EcoR V restriction enzyme digestion sites were introduced into both flanks of SH2 by overlapping extension PCR and the modified gene were cloned into pcDNA3.1(+). The pcDNA3.1(+)-CblN/Grb2 was obtained by replacing SH2 of CblN with Grb2SH2 and then used as templates to amplify CblN/Grb2 by PCR. The pGEX-4T-2-CblN/Grb2 was constructed by subcloning CblN/Grb2 into the prokaryotic expressing vector pGEX-4T-2. The GST-CblN/Grb2 fusion protein was expressed in E. coli of DH5? under IPTG induction and further purified with Glutathione Sepharose 4B. In vitro ubiquitination assay was performed to investigate whether the GST-CblN/Grb2 fusion protein is able to mediate auto-ubiquitinating reaction, namely whether it possesses ubiquitin ligase activity. Results The fusion expressing vector of pGEX-4T-2-CblN/Grb2 was successfully constructed; The GST-CblN/Grb2 fusion protein was correctly expressed and purified; In vitro ubiquitination assay indicated that GST-CblN/Grb2 fusion protein is able to mediate auto-ubiquitinating reaction and therefore possesses ubiquitin ligase activity. Conclusion Expression, purification of GST-CblN/Grb2 and identification of its′ activity have laid the foundation for further study of chimeric ubiquitin ligase′ effects on the growth of HER2 positive tumor cells.