Genome-wide identification of SUN gene family in Fragaria vesca and stresses-response analysis / 生物工程学报

SUN gene is a group of key genes regulating plant growth and development. Here, SUN gene families of strawberry were identified from the genome of the diploid Fragaria vesca, and their physicochemical properties, genes structure, evolution and genes expression were also analyzed. Our results showed that there were thirty-one FvSUN genes in F. vesca and the FvSUNs encoded proteins were classified into seven groups, and the members in the same group showed high similarity in gene structures and conservative motifs. The electronic subcellular localization of FvSUNs was mainly in the nucleus. Collinearity analysis showed that the members of FvSUN gene family were mainly expanded by segmental duplication in F. vesca, and Arabidopsis and F. vesca shared twenty-three pairs of orthologous SUN genes. According to the expression pattern in different tissues shown by the transcriptome data of F. vesca, the FvSUNs gene can be divided into three types: (1) expressed in nearly all tissues, (2) hardly expressed in any tissues, and (3) expressed in special tissues. The gene expression pattern of FvSUNs was further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the seedlings of F. vesca were treated by different abiotic stresses, and the expression level of 31 FvSUNs genes were assayed by qRT-PCR. The expression of most of the tested genes was induced by cold, high salt or drought stress. Our studies may facilitate revealing the biological function and molecular mechanism of SUN genes in strawberry.

Ecological strawberry production: promoting crop vitality with high-dynamized dilutions

The Strawberry (Fragaria× ananassaDuch.) is the world's most important berry. Around 9.2 million tonnes of strawberries were produced worldwide in 2021 over approximately 395,844 hectares distributed across almost all continents. However, industrial farming approaches, which include the application of high volumes of pesticides, have placed the fruit on the list of foods most contaminated by pesticide residues. Such management negatively affects food security and environmental sustainability. Agroecology is proposed as a holistic alternative tosolve this problem, and within this, some practices associated with homeopathy and biodynamic farming involve the application of high-dynamized dilutions as alternatives to chemical pesticides. Research indicates that the use of high-dynamized dilutions holds the potential to promote crop vitality through building natural equilibrium and resilience of agricultural systems. The objective of this research was to explore the extent to which high-dynamized dilutions can increase the sustainability of commercial strawberry production as well as understand the challenges and benefits of using high-dynamized dilutions in agriculture. To do this, natural and social science methods are combined in a multidisciplinary approach that was developed simultaneously in Brazil and the UK. Results of controlled trials demonstrated that the use of high-dynamized dilutions of Phosphorus12CH, Sulphur12 CH, and Kali Carbonicum12CH positively influenced crop production, pest disease levels, and plant vigor in strawberry plants.In addition, data collected from a web surveyand interviews with farmers, researchers,and advisors who work with homeopathy, evidenced the role of homeopathy and biodynamic farming as transformative tools regarding ecological awareness and ecological education, helping to advance the concept of the agriculture organism and subtle aspects of life into agricultural research and society.

Análise microbiológica de morangos frescos comercializados em Goiânia, Goiás / Microbiological analysis of fresh strawberries comercialized in Goiânia, Goiás

Objetivo: O consumo de alimentos contaminados por microrganismos patogênicos pode levar a quadros de doenças transmitidas por alimentos. O presente estudo objetivou realizar uma análise microbiológica de morangos frescos (Fragaria vesca) comercializados em Goiânia, Goiás, pesquisando a presença de Escherichia coli e Salmonella spp., em consonância com a RDC nº 12, de 02 de janeiro de 2001. Métodos: Foram analisadas 51 amostras de morangos frescos in natura no Laboratório Clínico da PUC Goiás, sendo 25 amostras oriundas de supermercados e 26 de comércio livre/rua, entre agosto/2019 e dezembro/2019, em diversos bairros das regiões Norte, Noroeste, Sul e Central do município de Goiânia, Goiás. Foram feitas culturas bacterianas nos ágares Eosin Methylene Blue e Salmonella-Shigella, e identificados os isolados por provas bioquímicas clássicas. Como controle de qualidade, a cada bateria de experimentos, duas cepas bacterianas conhecidas, uma Salmonella spp. (Programa Nacional de Controle de Qualidade/PNCQ) e uma E. coli ATCC 25923, foram utilizadas. Resultados: Das 51 amostras analisadas, 5,9% (3/51) apresentaram positividade para o crescimento de E. coli, sendo estas amostras adquiridas no comércio livre/rua. Foi observada ausência de Salmonella spp. Conclusão: A grande maioria (94,1%) das amostras analisadas nesta pesquisa negativaram em relação à presença de E. coli e 100% negativaram para a presença de Salmonella spp., demonstrando que independente de adquiridos em comércio livre/rua ou em supermercados, os morangos encontravam-se microbiologicamente seguros para o consumo, de acordo com a legislação brasileira vigente.

Objective: The consumption of food contaminated by pathogenic microorganisms can cause Foodborne Diseases. The present study aimed to perform a microbiological analysis of fresh strawberries (Fragaria vesca) marketed in Goiânia, Goiás, researching the presence of Escherichia coli and Salmonella spp., in line with RDC No. 12, of January 2, 2001. Methods: They have analyzed 51 samples of fresh strawberries in natura at the Clinical Laboratory of PUC Goiás, 25 samples from supermarkets and 26 from free trade/street, between August/2019 and December/2019, in several neighborhoods in the North, Northwest, South, and Central regions county of Goiânia, Goiás. Bacterial cultures cultivated on Eosin Methylene Blue and Salmonella-Shigella agar, and identification of the isolates by standard biochemical tests. As a quality control, in each battery of experiments, two known bacterial strains, one Salmonella spp. (National Quality Control Program/PNCQ) and an E. coli ATCC 25923 was used. Results: Of the 51 samples analyzed, 5.9% (3/51) were positive for the growth of E. coli, these samples were acquired in free/street commerce. Absence of Salmonella spp. Conclusion: The majority (94.1%) of the samples analyzed in this research were negative concerning the presence of E. coli, and 100% were negative for the presence of Salmonella spp., demonstrating that independent of being acquired in free trade/street or supermarkets, the strawberries analyzed were microbiologically safe for consumption, following the current Brazilian legislation.

Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.

Micorrhizal biotechnology as an alternative to potentialize the strawberry quality / Biotecnologia micorrízica como alternativa para potencializar a qualidade do morangueiro

The need to develop and use biotechnology tools to improve management and nutritional techniques in strawberry cultivation are increasing. Based on this, the objective of this study was to test if the mycorrhizal inoculation changes the agronomic and qualitative performance of strawberry fruits. The strawberry daughters plants used were of the cultivar Camarosa. The study was carried out in an agricultural greenhouse, Horticulture Sector of the Faculty of Agronomy and Veterinary Medicine (FAMV) of UPF, in the city of Passo Fundo, Rio Grande do Sul (RS), Brazil. The experiment was developed and maintained from June to December 2015. The treatments consisted of five inoculum: T1 = no inoculum (control); T2 = Acaulospora morrowiae; T3 = Rhizophagus clarus; T4 = mycorrhizal community; T5 = Claroideoglomus etunicatum. The experimental design was a randomized block with four replicates. Each plot consisted of a bag containing 6 plants spaced 0.15 mx 0.15 m. In the pits of the plants, it was applied, with the aid of syringe and water, about 70 infective propagules of the tested inocula. During the conduction of the experiment the temperature and photosynthetically active radiation (PAR) were monitored in the culture environment. It was evaluated the agronomic and qualitative performance of the fruits and the percentage of mycorrhizal colonization of the plants roots. The data were submitted to analysis of variance and the differences between means were compared by the Tukey test at 5% of probability error. The cultivar Camarosa produced more fruits in October. In September, the month before the one with the best agronomic performance, the mean temperature was 17.7 ºC. The mean PAR recorded in the growing environment was low in relation to crop requirements, throughout the growing period. Fruits produced by plants inoculated with A. morrowiae presented higher values of TSS/TTA ratio in September and October. The percentage of mycorrhizal colonization varied from 26.2% for A. morrowiae to 46.2% for R. clarus. The inoculation of arbuscular mycorrhizal fungi does not alter the production of strawberry fruits. However, when the plants are inoculated with A. morrowiae there is an improvement in the fruit flavor.

A necessidade de desenvolver e usar ferramentas biotecnológicas para melhorar o manejo e as técnicas nutricionais no cultivo do morangueiro está aumentando. Com base nisso, o objetivo desse estudo foi testar se a inoculação micorrízica altera o desempenho agronômico e qualitativo de frutos de morangueiro. As mudas de morangueiro usadas foram da cultivar Camarosa. O estudo foi realizado em estufa agrícola, no Setor de Horticultura da Faculdade de Agronomia e Medicina Veterinária (FAMV) da UPF, na cidade de Passo Fundo, Rio Grande do Sul (RS), Brasil. O experimento foi desenvolvido e mantido de junho a dezembro de 2015. Os tratamentos consistiram em cinco inóculos: T1 = sem inóculo (controle); T2 = Acaulospora morrowiae; T3 = Rhizophagus clarus; T4 = comunidade micorrízica; T5 = Claroideoglomus etunicatum. O delineamento experimental foi em blocos casualizados, com quatro repetições. Cada parcela consistiu de um saco contendo 6 plantas espaçadas de 0.15 mx 0.15 m. Na cova de plantio das plantas, foi aplicado, com auxílio de seringa e água, cerca de 70 propágulos infectivos dos inóculos testados. Durante a condução do experimento, a temperatura e a radiação fotossinteticamente ativa (PAR) foram monitoradas no ambiente de cultivo. Foi avaliado o desempenho agronômico e qualitativo dos frutos e a porcentagem de colonização micorrízica das raízes das plantas. Os dados foram submetidos à análise de variância e as diferenças entre médias foram comparadas pelo teste de Tukey, a 5% de erro de probabilidade de erro. A cultivar Camarosa produziu mais frutos em outubro. Em setembro, no mês anterior ao de melhor desempenho agronômico, a temperatura média foi de 17.7ºC. A PAR média registrada no ambiente de cultivo foi baixa em relação às necessidades da cultura durante todo o período de cultivo. Frutos produzidos por plantas inoculadas com A. morrowiae apresentaram maiores valores da relação SST/ATT em setembro e outubro. A porcentagem de colonização micorrízica variou de 26.2% para A. morrowiae a 46.2% para R. clarus. A inoculação de fungos micorrízicos arbusculares não altera a produção de frutos de morangueiro. No entanto, quando as plantas são inoculadas com A. morrowiae, há uma melhora no sabor dos frutos.

Pollination service of Nannotrigona testaceicornis stingless bees in strawberry / Serviço de polinização da abelha Nannotrigona testaceicornis no morangueiro

Strawberry presents moderate dependence on bee pollination service, and pollination is related to the production and quality of fruits. The aim of this study was to evaluate the pollination service provided by Nannotrigona testaceicornis stingless bees in strawberry. Primary flowers of cultivar San Andreas were used in a completely randomized experimental design, with twelve replicates and five types of pollination: one N. testaceicornis visit (1V); two N. testaceicornis visits (2V); three N. testaceicornis visits (3V); natural pollination (NP); self-pollination (SP). In flowers visited by N. testaceicornis, movements and visit time were observed. The following fruit characteristics were evaluated: fruit biometry, fecundation rate of achenes, postharvest fruit quality, contribution of pollination agents and mechanisms on average fruit weight. In fruit biometry, pollination service contributed only in longitudinal length, which was higher in NP and 3V, compared to SP. In the different types of pollination, the fertilization rate of achenes did not differ and showed no effect on fresh fruit weight. In post-harvest fruit quality, 1V, 3V and NP showed better results regarding degree of deformation and marketability. Nannotrigona testaceicornis stingless bees and natural pollination contributed to the average fresh weight of strawberry fruits. Nannotrigona testaceicornis stingless bees and natural pollination provided improvement in quality and added value of fruits. Nannotrigona testaceicornis stingless bees were effective strawberry pollinators.(AU)

O morangueiro apresenta dependência moderada quanto ao serviço de polinização por abelhas, e sua polinização está relacionada com a produção e a qualidade das frutas. Diante disso, o objetivo deste estudo foi avaliar o serviço de polinização da abelha Nannotrigona testaceicornis no morangueiro. Utilizaram-se flores primárias do cultivar San Andreas no delineamento experimental inteiramente casualizado, com doze repetições e cinco tipos de polinização: uma visita de N. testaceicornis (1V); duas visitas de N. testaceicornis (2V); três visitas de N. testaceicornis (3V); polinização natural (PN) e autopolinização (AP). Os movimentos e o tempo de visita nas flores foram observados nas visitas de N. testaceicornis. Nas frutas, avaliaram-se as características: biometria das frutas, taxa de fecundação dos aquênios, qualidade pós-colheita, contribuição de agentes e mecanismos de polinização na massa média das frutas. Na biometria das frutas, o serviço de polinização contribuiu no comprimento longitudinal, que se apresentou mais elevado na PN e 3V, em relação à AP. Nos diferentes tipos de polinização, a taxa de fecundação dos aquênios não diferiu e não apresentou efeito sobre a massa fresca das frutas. Na qualidadepós-colheita, 1V, 3V e PN apresentaram melhores resultados no grau de deformação e frutas comercializáveis. A abelha N. testaceicornis e a polinização natural contribuíram na massa fresca média das frutas de morangueiro. Os serviços de polinização natural e de N. testaceicornis proporcionaram melhoria na qualidade e agregação de valor das frutas. A abelha N. testaceicornis apresentou-se como polinizadora efetiva do morangueiro.(AU)

Efecto de recubrimientos comestibles de Aloe vera y alginato de sodio sobre la calidad poscosecha de fresa / Effect of Aloe vera and sodium alginate edible coatings on postharvest quality of strawberry

RESUMEN Las fresas son frutas altamente perecederas, susceptibles a daño mecánico, deterioro y desórdenes fisiológicos durante su almacenamiento. La aplicación de recubrimientos, se presenta como una alternativa promisoria de tratamiento poscosecha, que permite mantener la calidad de las fresas. El objetivo de esta investigación fue estudiar el efecto de recubrimientos comestibles de sábila (A. vera) y alginato de sodio sobre parámetros de calidad de fresas, durante el almacenamiento refrigerado. Se evaluaron mezclas de recubrimientos comestibles de alginato de sodio y A. vera (100:0, 75:25, 50:50 y 25:75) sobre la pérdida de peso, color, firmeza, pH y acidez titulable de fresas, durante almacenamiento refrigerado (0, 3 ,9 y 12 días). Adicionalmente, se determinó el espesor y la opacidad de las películas obtenidas a partir de las soluciones formadoras de recubrimientos, utilizando el método de "casting". Los resultados mostraron que la combinación de A. vera y alginato de sodio tiene un efecto significativo en la reducción de pérdida de calidad de fresa, durante el almacenamiento en refrigeración. Todos los tratamientos con recubrimientos comestibles presentaron, al final del almacenamiento, menor pérdida de peso (7-16%), mayor firmeza (entre 1,3 a 2,1 veces más), mayor retención del color y mayor acidez titulable que las muestras sin recubrimiento (control). El espesor de las películas disminuyó con el incremento de la adición de Aloe vera y la opacidad disminuyó significativamente con la adición de la sábila en 25% y 50% en las mezclas con alginato de sodio.

ABSTRACT Strawberries are highly perishable fruits, being susceptible to mechanical injury, decay and physiological disorders during storage. Applications of edible coatings have been shown to be promising as a tool to improve the quality and extend storage. The aim of this study was to evaluate the use of Aloe vera and sodium alginate as an edible coating in postharvest quality of cold storage strawberries. The composition of edible coatings of sodium alginate and A. vera (100: 0, 75:25, 50:50 and 25:75) was studied on weight loss, color, firmness, pH and titrable acidity of strawberries during refrigerated storage (0, 3, 9 and 12 days). The thickness and opacity of the films were also determined, from the coating forming solutions using the casting method. The short shelf life and the high deterioration of the quality characteristics of strawberries during postharvest, justify to investigate alternative conservation techniques such as edible coatings, in order to reduce food loss and improve consumer acceptance The results showed that the combination of Aloe vera and sodium alginate has a significant effect in the reduction of the loss of the quality of the fruit during refrigerated storage. All coating treatments showed at the end of storage less weigth loss (7-16%), greater firmness, (between 1,3 to 2,1 more times) more color retention and greater titrable acidity tan uncoated samples (Control). The addition of Aloe vera decreased the thickness of the films and the opacity decreased significantly with the addition of Aloe vera in 25 and 50% in the mixtures with sodium Alginate.

Efecto del tratamiento y tiempo de almacenamiento sobre los compuestos funcionales de subproductos de mora y fresa / Effect of treatment and storage time on the functional compounds of blackberry and strawberry by-products

RESUMEN Cerca de un tercio de las partes comestibles de los alimentos para el consumo humano se pierden, siendo mermas asociadas a factores de toda la cadena de abastecimiento. Dentro de dichos alimentos, se destacan la fresa (Fragaria ananassa) y la mora (Rubus glaucus Benth), frutas que han demostrado alto poder antioxidante, siendo relacionado con la prevención o el tratamiento de enfermedades. Por consiguiente, con el propósito de evidenciar el potencial para la obtención de compuestos de interés en los subproductos del procesamiento de dichas frutas, el presente estudio, se enfocó en la caracterización de los parámetros fisicoquímicos, color, poder antioxidante y vitamina C, en cuatro condiciones de conservación, durante cuatro semanas; estas fueron: coproducto fresco, refrigerado, secado convencional y liofilización, siendo caracterizadas también por su contenido de minerales. A partir de los resultados, se evidenció baja concentración de sólidos solubles, pH variable, entre 3,2 y 6,4, así como parámetros de color estables, durante el almacenamiento. Adicionalmente, los coproductos con mayor poder antioxidante fueron la semilla de mora liofilizada y el lodo de mora por secado convencional, superando al residuo de fresa en cualquier condición, según los ensayos FRAP y DPPH, respectivamente; no obstante, todas las muestras presentaron bajos contenidos en vitamina C, mientras que se caracterizaron por su alto contenido en potasio y hierro. Por consiguiente, lo anterior, muestra el interés por la obtención de compuestos, como minerales y antioxidantes, a partir de los desechos de mora y de fresa, así como su uso en productos con alto valor agregado.

ABSTRACT Near of one third of the edible food that human being cultivate are loses, which are due to factors from the supply chain. Within these was highlighted strawberry (Fragaria ananassa) and blackberry (Rubus glaucus Benth), fruits that have demonstrated high antioxidant potential to which is related with prevention of some diseases. Therefore, with the aim to show the processing by-products' potential in the obtaining of interesting compounds, this study was focused on the characterization of their physicochemical parameters, color, antioxidant power and vitamin C in four preservation conditions for four weeks; these were: fresh by-product, refrigerated, conventional drying and lyophilization; also, mineral description was made for each sample. According to the results, it was proved that the samples had a lower solid soluble concentration, pH between 3.2 and 6.4, and stable color parameters during the storage. In addition, by-products with greater antioxidant power were lyophilizate blackberry seed and conventional drying blackberry sludge, which surpassed the strawberry's results in all the conditions conforming to FRAP and DPPH essays. However, all the samples presented low content of vitamin C, while they exhibited high content of potassium and iron. To sum up, above mentioned woke the interest on the minerals or antioxidant obtention from blackberry and strawberry by-products to which can be used in products with high value added.

Potential of endophytic fungi as biocontrol agents of Duponchelia fovealis (Zeller) (Lepidoptera: Crambidae) / Fungos endofíticos para o biocontrole de Duponchelia fovealis (Zeller) (Lepidoptera: Crambidae)

Abstract This study reports the first assessment of endophytic fungi isolated from strawberry leaves and selection of isolates for the control of Duponchelia fovealis, a new pest of strawberries. A total of 400 strawberry leaves of the cultivar 'Albion' were collected in four commercial farms. Leaves were disinfected, cut in fragments, and placed on Petri dishes containing potato dextrose agar media with tetracycline and incubated for 30 days. Following this time, 517 fungal colonies were isolated, and thirteen genera were identified: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe, and Phoma. Eight isolates belonging to the genera Aspergillus, Diaporthe, Paecilomyces, and Cladosporium were selected for pathogenicity bioassays against third instar larvae of D. fovealis. Isolates of Paecilomyces induced the highest mortality rates.

Resumo Este trabalho apresenta a primeira contribuição no isolamento de fungos endofíticos de folhas de morangueiro e na seleção de isolados para controle de Duponchelia fovealis, uma nova praga do morangueiro. Foram coletadas 400 folhas da cultivar 'Albion' em quatro lavouras comerciais de morangueiro. As folhas foram desinfetadas, cortadas em fragmentos e depositadas em placas de Petri contendo Ágar Batata Dextrose como meio, em conjunto com tetraciclina e incubados durante 30 dias. Um total de 517 colônias fúngicas e treze gêneros foram isolados: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe e Phoma. Oito isolados pertencentes aos gêneros Aspergillus, Diaporthe, Paecilomyces e Cladosporium foram selecionados para os bioensaios de virulência contra larvas de 3º instar de D. fovealis. Isolados de Paecilomyces causaram as maiores taxas de mortalidade.

Neofusiccocum parvum as Uncommon Fungal Species (or Emerging Pathogen) on Strawberry Plants in Morocco

During a survey on fungi associated with decline symptoms on strawberry plant of Venicia variety, one species belonging to the Botryosphaeriaceae family was isolated. Based on morphological and cultural characteristics, this species identified as Neofusicoccum parvum was reported for the first time in Morocco. To verify the pathogenicity of the fungus, detached leaves of three strawberry varieties were inoculated artificially by depositing over their intact surface mycelia plug or conidial suspension from N. parvum. Severity index was greater on festival leaves reaching 88% compared to 77.73% on Sabrina. In the third treatment, Guariguette showed a low susceptibility with a severity index in order of 25.07%. Conidia concentration on the leaf surface of the Festival and Sabrina strawberry leaves was respectively 1.62 105and 1.2 105 conidia cm-2. Otherwise, in the second treatment, it has been reduced to less than 1.41105 1.16 and 105 conidia.cm-2 on leaves of Festival and Sabrina respectively. After inoculation, the fungus was re-isolated from the lesions to verify Koch’s postulates.

Linking the platelet antiaggregation effect of different strawberries species with antioxidants: metabolomic and transcript profiling of polyphenols / Asociando el efecto de antiagregación plaquetaria de distintas especies de frutillas con antioxidantes: perfil metabolómico y transcriptómico de polifenoles

A comparative study of antioxidant properties, platelet antiaggregation activity and transcriptional analysis of flavonoid biosynthesis genes were performed in Fragaria x ananassa, F. vesca and F. chiloensis subsp chiloensis f. chiloensis and f. patagonica. Furthermore, differences in flavonoid content were found by UHPLC-MS. The highest free radical scavenging activity by DPPH assay was observed in F. chiloensis f. chiloensis, meanwhile, F. vesca presented the highest antioxidant capacity by FRAP. Biosynthetic flavonoids- related transcripts were higher abundant in F. x ananassa and lower in F. vesca. Additionally, all strawberry extracts showed antiaggregant effect (1 mg mL-1), but F. vesca and F. chiloensis subsp. chiloensis f. patagonica were still active at lower concentration. This study suggests that platelet antiaggregation effect of different strawberries could be due to isoflavones and flavonoids precursors in addition to anthocyanins. Results could usefully to take decisions in future breeding programs to improve the content of healthy compounds in strawberry fruits.

Se realizó un estudio comparativo de propiedades antioxidantes, actividad de antiagregacion plaquetaria, análisis transcripcional de genes de biosíntesis de flavonoides y contenido de estos en Fragaria x ananassa, F. vesca and F. chiloensis subsp chiloensis f. chiloensis and f. patagonica. La mayor actividad removedora de radicales libres por DPPH se observó en F. chiloensis f. chiloensis, mientras F. vesca presentó la mayor capacidad antioxidante mediante FRAP. Transcritos relacionados con biosíntesis de flavonoides fueron mas abundantes en F. x ananassa y menores en F. vesca. Adicionalmente, todos los extractos de frutillas mostraron efectos antiagregante (1 mg mL-1), pero F. vesca and F. chiloensis subsp. chiloensis f. patagonica fueron activos a concentraciones menores. Este estudio sugiere que efectos de antiagregación plaquetaria en distintas frutillas podría deberse a isoflavonas y precursores de flavonoides además de antocianinas. Los resultados podrían ser útiles en programas de mejoramiento genético para mejorar el contenido de compuestos saludables en frutilla.

Genetic divergence of strawberry cultivars under different managements / Divergência genética de cultivares de morango em diferentes manejos

Developing strawberry cultivars that can be grown on a large scale, it is necessary to gather desirable characteristics such as: tolerance to Tetranychus urticae, high fruit yield and wide adaptability to several cropping managements. Therefore, our objective was to study the genetic diversity among 13 strawberry cultivars under different managements and to recommend promising crosses to obtain segreganting populations with high fruit yield and T. urticae tolerance. Trial was performed under field conditions at the Centro Regional de Desenvolvimento Rural Centro Serrano of the Instituto Capixaba for Technical Assistance and Rural Extension (Incaper), Domingos Martins-ES. We evaluated strawberry cultivars Albion, Aleluia, Aromas, Camarosa, Camino Real, Campinas, Diamante, Dover, Festival, Seascape, Toyonoka, Tudla, and Ventana, cultivated in three cropping managements: open field, low tunnel and high tunnel. Experimental design was randomized complete blocks with three replications. Variables evaluated were: number of two-spotted spider mite/cm2 on the leaf (NTSSM), total number of fruits (TNF), number of commercial fruits (NCF) and fruit yield (YIE, t/ha). We applied the generalized Mahalanobis distance and Tocher's optimization method to study the genetic diversity among cultivars in each management, and the relative contribution of traits to genetic diversity was evaluated according to the criterion described by Singh (1981). For the low tunnel and high tunnel environments, the crosses Aleluia x Camarosa, Aleluia x Aromas and Aleluia x Festival are the most promising to generate segregating populations with a higher possibility to appearance transgressive individuals, while for the open field cultivation system, we recommend the cross among Aleluia x Toynoka. The variables that most contributed for genetic dissimilarity were total number of fruits, fruit yield and number of commercial fruits for the environments open field, low tunnel and high tunnel, respectively.

´Para desenvolver cultivares de morango que podem ser cultivados em larga escala é necessário reunir características desejáveis como: tolerância ao Tetranychus urticae, alta produtividade de frutos e ampla adaptabilidade a diversos sistemas de cultivo. Portanto, o objetivo do trabalho foi estuda a diversidade genética entre 13 cultivares de morango sob diferentes manejos e recomendar cruzamentos promissores para obtenção de populações segregantes com alta produtividade de frutos e tolerantes ao T. urticae. O experimento foi conduzido sob condições de campo no Centro Regional de Desenvolvimento Rural Centro Serrano do Instituto Capixaba de Assistência Técnica e Extenção Rural (Incaper), Domingos Martins-ES, no mês de outubro (primavera). Foram avaliadas as cultivares Albion, Aleluia, Aromas, Camarosa, Camino Real, Campinas, Diamante, Dover, Festival, Seascape, Toyonoka, Tudla e Ventana, cultivadas em três sistemas de cultivo: campo aberto, túnel baixo e túnel alto. O delineamento experimental utilizado foi blocos casualizados com três repetições. As variáveis avaliadas foram: número de ácaro/cm² na folha (NTSSM), número total de frutos (TNF), número de frutos comerciais (NCF) e produtividade de frutos (YIE, t/ha). Foram empregadas a distância generalizada de Mahalanobis e o método de otimização de Tocher para o estudo da diversidade genética entre os cultivares em cada manejo, e a contribuição relativa dos caracteres para a diversidade genética foi avaliada segundo o critério de Singh (1981). Para os manejos túnel baixo e túnel alto, os cruzamentos entre os cultivares Aleluia x Camarosa, Aleluia x Aromas e Aleluia x Festival são os mais promissores para gerar populações segregantes com alta possibilidade de aparecimento de indivíduos transgressivos, enquanto que para o campo aberto recomenda-se o cruzamento entre os cultivares Aleluia x Toynoka. As variáveis que mais contribuíram para a dissimilaridade genética foram o número total de frutos, produtividade e número de frutos comerciais para os ambientes campo aberto, túnel baixo e túnel alto, respectivamente.

Study of Inhibitory Effect of Polyphenols from Fragaria ananassa on Fat Accumulation in Rat White Adipocyte / 日本補完代替医療学会誌

On the study of polyphenols from Fragaria ananassa fruit, we reported that some polyphenols showed inhibition of metabolic enzyme, cytochrome P450. Continuous study of health effects of F. ananassa fruit, we isolated a new quercetin glycoside, flagarin, quercetin-3-O- β-glucuronyl- (2→1)- β-D-xyloside along with ten known compounds. Those compounds showed inhibitory activity of fat accumulation in rat white adipocyte. Among the isolated compounds, strictinin and the new compound, flagarin showed high inhibitory activity of fat accumulation in rat white adipocyte.

Potential of endophytic fungi as biocontrol agents of Duponchelia fovealis (Zeller) (Lepidoptera:Crambidae)

Abstract This study reports the first assessment of endophytic fungi isolated from strawberry leaves and selection of isolates for the control of Duponchelia fovealis, a new pest of strawberries. A total of 400 strawberry leaves of the cultivar Albion were collected in four commercial farms. Leaves were disinfected, cut in fragments, and placed on Petri dishes containing potato dextrose agar media with tetracycline and incubated for 30 days. Following this time, 517 fungal colonies were isolated, and thirteen genera were identified: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe, and Phoma. Eight isolates belonging to the genera Aspergillus, Diaporthe, Paecilomyces, and Cladosporium were selected for pathogenicity bioassays against third instar larvae of D. fovealis. Isolates of Paecilomyces induced the highest mortality rates.

Resumo Este trabalho apresenta a primeira contribuição no isolamento de fungos endofíticos de folhas de morangueiro e na seleção de isolados para controle de Duponchelia fovealis, uma nova praga do morangueiro. Foram coletadas 400 folhas da cultivar 'Albion' em quatro lavouras comerciais de morangueiro. As folhas foram desinfetadas, cortadas em fragmentos e depositadas em placas de Petri contendo Ágar Batata Dextrose como meio, em conjunto com tetraciclina e incubados durante 30 dias. Um total de 517 colônias fúngicas e treze gêneros foram isolados: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe e Phoma. Oito isolados pertencentes aos gêneros Aspergillus, Diaporthe, Paecilomyces e Cladosporium foram selecionados para os bioensaios de virulência contra larvas de 3º instar de D. fovealis. Isolados de Paecilomyces causaram as maiores taxas de mortalidade.

Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L) Duch. )

BACKGROUND: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. RESULTS: In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3-6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. CONCLUSIONS: For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.

Biological parameters and fertility life table of Aphis forbesi Weed, 1889 (Hemiptera: Aphididae) on strawberry / Parâmetros biológicos e tabela de vida de fertilidade de Aphis forbesi Weed, 1889 (Hemiptera: Aphididae) em morangueiro

Abstract This study provides the first contribution of the biology and life table of Aphis forbesi Weed, 1889 (Hemiptera: Aphididae), an important strawberry pest throughout the world. This species lives in the crown and leaf petioles of the plant. It is difficulty to rear this species in laboratory due to protocooperation with ants observed only in the field. We studied the life cycle of A. forbesi on the leaves of the Albion strawberry cultivar at 25 ± 2 °C, 60 ± 10% relative humidity, and a 12-h photophase. The experiment was randomised with 100 replicates. The parameters of the fertility life table were calculated using TabVida. In the population studied, 25% and 46% had four and three instars, respectively. A mean of 1.43 nymphs per female per day was generated. The mean reproductive period was seven days and the mean longevity was 10 days. In every 11 days there is a generation of A. forbesi, where each female has the potential to generate between 6 to 9 individuals daily, increasing its population by 1.2 times. The average life cycle was 16.8 days. High viability observed in all instars and the resulting values of R0, rm and λ suggest that A. forbesi has the capacity to increase their numbers in a short period of time, while generating high populations in strawberry crops, requiring differential management.

Resumo Este trabalho apresenta a primeira contribuição ao estudo de biologia e tabela de vida de fertilidade de Aphis forbesi, Weed, 1889 (Hemiptera: Aphididae), uma importante praga de morangueiro no mundo. Esta espécie se desenvolve na coroa e pecíolo do morangueiro. O desenvolvimento desta espécie em laboratório apresentou dificuldades, possivelmente devido à protocooperação com formigas, observada em campo durante coletas. O ciclo de vida de A. forbesi foi estudado em folhas de morangueiro cultivar ‘Albion’ a 25 ± 2 °C, 60 ± 10% umidade relativa, e fotofase de 12 horas. O experimento foi inteiramente casualizado com 100 repetições. Os parâmetros da tabela de vida de fertilidade foram calculados usando o software TabVida. Na população estudada observou-se que as ninfas apresentaram três e quatro instares, sendo 46 e 25% respectivamente, dos indivíduos que completaram o ciclo de vida. Foi gerada uma média de 1,43 ninfas/ fêmea/ dia. O período reprodutivo médio foi de 7 dias e a longevidade média 10 dias. A cada 11 dias ocorre uma geração de A. forbesi onde cada fêmea tem capacidade de gerar de 6 a 9 indivíduos aumentando em 1,2 vezes a população. O ciclo de vida de A. forbesi durou em média 16,8 dias. A alta viabilidade observada em todos os estádios, e os valores de R0, rm e λ sugerem que A. forbesi tem a capacidade de aumentar seu número em um curto período de tempo, gerando altas populações no cultivo do morangueiro, exigindo manejo diferenciado.

Applications of 5-aminolevulinic acid on the physiological and biochemical characteristics of strawberry fruit during postharvest cold storage / 5-aminolevulínico ácido sobre as características fisiológicas e bioquímicas de morango

ABSTRACT: The compound 5-aminolevulinic acid (ALA) is a key precursor in the biosynthesis of porphyrins, such as chlorophyll, heme and phytochromobilin, and has multiple physiological effects on plants. Varying concentrations of ALA (50mg L-1, 100mg L-1, and 150mg L-1) and water (control) were applied to white stage 'Sweet Charlie' strawberry fruit. All ALA treatments delayed senescence and improved the qualities of strawberries fruit during storage. Among the treatments, 150mg L-1 ALA was the most effective dosage concentration. Exogenously applied ALA significantly reduced the decay index, respiration rate, O2 - production rate (O2 -), H2O2 and malondialdehyde (MDA) content, increased superoxide dismutase (SOD), ascorbate peroxidase activities (APX), total soluble solids (TSS) content, titratable acidity (TA) and anthocyanin content during the initial stage of storage. These results supported the pre harvest application of ALA as a beneficial strategy for the prevention of postharvest decay of strawberry fruit.

RESUMO: O composto de ácido 5-aminolevulínico (ALA) é um precursor chave na biossíntese de porfirinas, tais como clorofilae porfirinas, e verificou-se induzir elevações temporárias na taxa de fotossíntese e APX. Além disso, ele tem vários efeitos fisiológicos sobre os vegetais. Após o tratamento, Ala (50mg L-1, 100mg L-1 e 150mg L-1) ou água (controlo) foi aplicado a frutos maduros branco "Sweet Charlie" de morango. As atividades índice de decadência, taxa de respiração, superóxido dismutase e ascorbato peroxidase, conteúdo de sólidos solúveis, acidez titulável, teor de antocianinas, taxa de produção de O2-, e conteúdo malondialdehyde foi avaliada nos frutos ALA-tratados e morango controle durante a armazenagem a 4°C. Os resultados mostraram que a aplicação exógena de ALA retarda a senescência e melhorou o valor nutricional das frutas de morango durante o armazenamento.

Estimated phyllochron in low tunnel cultivated strawberry cultivars / Estimativa de filocrono em cultivares de morangueiro conduzidas em túnel baixo

ABSTRACT: The aim of this study was to determine phyllochron in strawberry cultivars, from two origins during two crop years, conducted in low tunnel. Experiments were conducted at the Universidade Federal de Santa Maria, campus Frederico Westphalen (CESNORS/UFSM), Brazil, evaluating 'Camarosa', 'Camino Real' and 'Albion' strawberry cultivars. During the 2012 crop year, seedlings from Chile and Pelotas/RS, Brazil were evaluated, for the 2013 crop year, only seedlings from Chile were submitted to evaluations. In order to determine the phyllochron, twice a week the numbers of leaves (NL) of the main crown were counted and a regression between NL and accumulated thermal time (ATT) was held. The experimental design was a complete randomized block, with three repetitions with the evaluation of 12 plants per experimental unity, consisting of a factorial. For 2012 and 2013 crop years, it is possible to conclude that 'Camarosa' and 'Camino Real' cultivars required higher accumulation of degree-days (ºC day-1) to issue each successive leaf than cv. 'Albion', which, in turn, presented higher rates of leaf emergence, being considered earlier. Regarding to origin, seedlings from cv. 'Albion' from Chile presented lower total phyllochron values and for samples from Pelotas, no difference among cultivars was observed.

RESUMO: O objetivo deste trabalho foi determinar o filocrono, em cultivares de morangueiro, de duas procedências e em dois anos agrícolas, conduzidas em túnel baixo. Os experimentos foram conduzidos em área da Universidade Federal de Santa Maria, campus Frederico Westphalen (CESNORS/UFSM), constando da avaliação das cultivares 'Camarosa', 'Camino Real' e 'Albion'. Para o ano de 2012, avaliaram-se mudas procedentes do Chile e da região de Pelotas/RS e, para o ano de 2013, apenas mudas chilenas. Na determinação do filocrono, foi realizada contagem, duas vezes por semana, do número de folhas (NF) da coroa principal e, posteriormente, realizou-se regressão linear entre o NF e a soma térmica acumulada (STa). O delineamento experimental foi o de blocos casualizados, com três repetições, sendo cada unidade experimental composta por 12 plantas de avaliação, em esquema fatorial. Para os anos de 2012 e 2013, os resultados obtidos mostram que as cultivares 'Camarosa' e 'Camino Real' necessitam de maior acúmulo de graus dia para emissão de cada folha sucessiva do que a cv. 'Albion', que, por sua vez, apresenta maiores taxas de emissão de folhas, podendo ser considerada como mais precoce. Com relação à procedência, verifica-se que as mudas da cv. 'Albion' provenientes do Chile apresentam menores valores de filocrono total. Para a procedência Pelotas, não foi observada diferença entre as cultivares.

Relationship between production, nematodes and "redness" in strawberries / Relação entre produção, nematoide e o sintoma de "vermelhão" em morangueiros

ABSTRACT: In recent years "redness" has increasingly appeared in strawberry plants with leaves taking on a reddish color. No causal agent has been associated with plants. Since strawberries presented problems due to the incidence of nematodes, the purpose of this study was to look at the relationship between production, resistance to the Meloidogyne hapla nematode and the "redness" symptom in strawberry cultivars. Two experiments were performed, both with the 'Camino Real', 'Festival', 'Oso Grande', 'Albion' and 'Camarosa' cultivars. The first experiment was performed in the field, where the following were evaluated: strawberry production, fruit quality, macro and micronutrient contents in fruit and leaves, percentage of plant survival, incidence of nematodes, quantity of eggs in the roots and juveniles in the soil, and the incidence of Botrytis cinerea . In the second experiment, the strawberries were transplanted into pots and filled with pinus bark-based commercial substrate. Half the pots were inocculated with Meloidogyne hapla . Cultivars presented differences in fruit production and also in the incidence of "redness". Lowest performance in production was related to the high incidence of the nematode Meloidogyne hapla. 'Oso Grande' and 'Albion' presented nematode-resistant behavior. It was possible find a relationship between the incidence of the Meloidogyne hapla nematode, and the incidence of "redness" only 'Camino Real' cultivar.

RESUMO: Nos últimos anos, vem aumentando o aparecimento do "vermelhão" nos morangueiros, que ficam com as folhas avermelhadas. Nenhum agente causal foi associado às plantas. Devido aos plantios de morangueiros apresentarem problemas pela incidência de nematoide, o objetivo do presente trabalho foi verificar a relação entre produção, resistência ao nematoide Meloidogyne hapla e o sintoma de "vermelhão" em cultivares de morangueiro. Foram realizados dois experimentos, ambos com as cultivares 'Camino Real', 'Festival', 'Oso Grande', 'Albion' e 'Camarosa'. O primeiro experimento foi realizado a campo, onde se avaliaram a produção de morangos, qualidade dos frutos, teores de macro e micronutrientes em frutos e folhas, porcentagem de sobrevivência das plantas, incidência de nematoides, quantidade de ovos nas raízes e juvenis no solo e a incidência de Botrytis cinerea. No segundo experimento, os morangueiros foram transplantados em vasos e preenchidos com substrato comercial a base de casca de pinus. Metade dos vasos foi inoculada com Meloidogyne hapla. As cultivares apresentaram diferenças na produção de frutos e também na incidência de "vermelhão". O menor desempenho produtivo relaciona-se com a alta incidência de nematoide Meloidogyne hapla. 'Oso Grande' e 'Albion' se comportaram como resistentes ao nematoide. Foi possível relacionar a incidência de nematoide Meloidogyne hapla com a de "vermelhão" apenas na cultivar 'Camino Real'.