Investigation of the population diversity of intermediate host snails and crabs of Paragonimus along Jiulong River, Zhangjiang River and Dongxi River basins in southern Fujian Province / 中国血吸虫病防治杂志

Objective To investigate the population distribution of intermediate host snails and crabs of Paragonimus along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in Bopingling Mountain, southern Fujian Province, so as to provide baseline data for researches on parasitic disease prevention and control and enlargement of samples in the parasitic resource bank. Methods A total of 23 villages in 8 counties (districts) along the Jiulong River, Zhangjiang River, and Dongxi River basins in Zhangzhou City, Fujian Province were selected as survey sites during the period from November 2020 through March 2023, and snail and freshwater crabs were sampled from 1 to 3 streams and ditches neighboring residential areas in each village. Morphological identification of snails was performed according to the external morphological characteristics of collected snail shells, and the unidentified snail species sampled from the natural foci of paragonimiasis in Yunxiao County were subjected to se-quence analysis of the mitochondrial cytochrome oxidase 1 (CO1) gene. The crab species was identified by observing the morphological characteristics of the terminal segment of the first pleopod of male crabs, and Paragonimus cercariae and metacercariae were detected in collected snails. Results The shells of the unidentified snails sampled from the natural foci of paragonimiasis in Yunxiao County were approximately 50 mm in height and 18 mm in width, thick and solid, long tower cone-shaped, and had 8 to 10 whorls. CO1 gene sequence analysis identified the snail species as Sulcospira hainanensis. A total of 6 freshwater snail species belonging to 5 genera within 3 families, identified 23 survey sites, including Semisulcospira libertina, Paludomus zhangchouensis and S. hainanensis that belonged to the Family Pleurceridae, Tricula fujianensis and T. huaanensis that belonged to the subfamily Triculinae, Family Pomatiopsidae, and Melanoides tuberculata (Family Thiaridae), and 11 species of freshwater crabs belonging to 5 genera within 2 families were identified, including Sinopotamon genus of S. jianglense, S. pinheense, and S. zhangzhouense, Huananpotamon genus of H. planopodum and H. zhangzhouense, Nanhaipotamon genus of N. huaanense and N. longhaiense, and Minpotamon genus of M. nasicum and M. auritum that belonged to the Family Potamidae, and Somanniathelphusa genus of S. huaanensis and S. zhangpuensis (Family Parathelphusidae). In addition, the prevalence of P. westermani cercariae infections was 0.08% (2/2 317) in P. zhangchouensis from Danyan Village in Changtai District and 0.09% (1/1 039) in S. hainanensis from Jinkeng Village in Yunxiao County, and the prevalence of P. westermani metacercariae infections was 25.81% (8/31) in S. jianglense from Danyan Village in Changtai District, and 26.31% (5/19) in S. zhangzhouense from Jinkeng Village in Yunxiao County, respectively. Conclusions There is a population diversity in the intermediate host snails and crabs along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in Bopingling Mountain, southern Fujian Province, and P. zhangzhouensis and S. hainanensis are, for the first time, confirmed as the first intermediate hosts of P. westermani.

Fish fauna of the Río San Pedro and Río La Pasión, Usumacinta River Basin, Guatemala / Ictiofauna del Río San Pedro y Río La Pasión, Usumacinta River Basin, Guatemala

Abstract The Río San Pedro and Río La Pasión lie within the Usumacinta River Basin, a globally significant center of freshwater fish diversity. Both rivers are listed among Central America's top 50 regions for conserving freshwater biodiversity. This study presents an updated checklist of 70 fish species, of which six are non-native to the Usumacinta River Basin. From these species, 69 are reported from the Río La Pasión and 56 reported from the Río San Pedro, representing higher species richness than previously reported. The checklist derives from a systematic survey of fishes conducted in 2019 and records available in public databases and published literature. Seventy-eight percent of the species were reported in both rivers, and Cichlidae and Poeciliidae had the most species. Secondary species represent 59% of the species reported, followed by peripheral species with 22% of the species. The species with highest fidelity in Río La Pasión were the armored catfish Pterygopichthys spp. and the livebearer Gambusia sexradiata; and the cichlids Thorichthys meeki and Oscura heterospila had highest fidelity in the Río San Pedro. Thorichthys helleri was widely distributed in both rivers. According to the IUCN Red List of Threatened Species, there are four species classified as Vulnerable in Río La Pasión. However, 62% of the species are of Least Concern, 25% of the species are Data Deficient, and 6% are listed as Not Evaluated. More research is needed to document the status of the fish fauna, and improved habitat protection is required to conserve stocks.

Resumen El río San Pedro y el río La Pasión se encuentran dentro de la cuenca del río Usumacinta, un centro de diversidad de peces de agua dulce de importancia mundial. Ambos ríos figuran entre las 50 regiones principales de América Central para conservar la biodiversidad de agua dulce. Este estudio presenta un listado actualizado de 70 especies de peces, de las cuales 6 son especies no nativas para la cuenca del Río Usumacinta. De estas especies, 69 se reportan para el Río La Pasión y 56 para el río San Pedro, lo que representa una riqueza de especies más alta que la reportada previamente. La lista de verificación se deriva de un muestreo sistemático de peces realizado en 2019 y registros disponibles en bases de datos públicas y literatura publicada. El 78% de las especies se reportan en ambos ríos, siendo Cichlidae y Poeciliidae las familias con mayor riqueza. Las especies con mayor fidelidad en Río La Pasión fueron el bagre acorazado Pterygopichthys spp. y el pez vivíparo Gambusia sexradiata; y los cíclidos Thorichthys meeki y Oscura heterospila tuvieron mayor fidelidad en el Río San Pedro. Thorichthys helleri se distribuyó ampliamente en ambos ríos. Según la Lista Roja de Especies Amenazadas de la UICN, existen cuatro especies clasificadas como Vulnerable en el Río La Pasión. Sin embargo, el 62 % de las especies son de Preocupación Menor, el 25 % de las especies tienen Datos Insuficientes y el 6 % se enumeran como No Evaluadas. Se necesita más investigación para documentar el estado de la fauna de peces, y se requiere una mejor protección del hábitat para conservar las poblaciones.

Metazoan parasite fauna of detritivorous and omnivorous fishes from Amapá Lake, in western Brazilian Amazon / Fauna parasitária de metazoários em peixes detritívoros e onívoros do Lago Amapá, na Amazônia Ocidental brasileira

Abstract Despite being an important component of the freshwater ecosystems and an important factor controlling wild fish populations, Amazonian fish parasites have been poorly studied. Here, we analyzed the fauna of metazoan parasites from 10 detritivorous and omnivorous fish species from Amapá Lake in the western Amazon. Of 159 fish specimens examined, 73.6% were parasitized by one or more species of metazoan parasites. A total of 5,260 parasites (24 Monogenea, 14 Nematoda, 10 Digenea, 2 Acanthocephala, and 6 Crustacea) were identified. Aggregated dispersion pattern of parasites was observed. There was a predominance of endohelminth species that presented a similar pattern regarding the level of component communities (i.e., a low species number, low diversity and dominance of a single species, mainly the digeneans and nematodes in their larval stages). Five species of parasites presented correlation with the body size of the hosts. In the fish community, the presence of endoparasites in the larval and adult stages suggests that these are intermediate, paratenic and definitive hosts, reflecting their detritivorous or omnivorous feeding habits. The trophic level and diet had an influence on the structuring of endohelminth communities and infracommunities. Lastly, 38% of the metazoan parasite species were new records for examined hosts.

Resumo Apesar de ser um importante componente dos ecossistemas de água doce e um importante fator controlador das populações naturais de peixes, os parasitos de peixes amazônicos ainda são pouco estudados. Foi analisada a fauna de parasitos metazoários em 10 espécies de peixes detritívoros e onívoros do Lago Amapá na Amazônia ocidental. Dos 159 espécimes de peixes examinados, 73,6% estavam parasitados por uma ou mais espécies de parasitos metazoários. Um total de 5.260 parasitos (24 Monogenea, 14 Nematoda, 10 Digenea, 2 Acanthocephala e 6 Crustacea) foram identificados. Houve um padrão de dispersão agregado de parasitos. Houve predominância de espécies de endohelmintos que apresentaram padrão semelhante quanto ao nível de comunidades componentes, ou seja, baixo número de espécies, baixa diversidade e dominância de uma única espécie, principalmente os digenéticos e nematoides em estágio larval. Cinco espécies de parasitos apresentaram correlação com o tamanho corporal dos hospedeiros. Na comunidade de peixes, a presença de endoparasitos em fase larval e adulta sugere que esses são hospedeiros intermediários, paratênicos e definitivos, refletindo seus hábitos alimentares detritívoros ou onívoros. O nível trófico e dieta influenciaram na estruturação das comunidades e infracomunidades de endohelmintos. Por fim, 38% das espécies de parasitos metazoários foram novos registros para os hospedeiros examinados.

Comparación ecotoxicológica al Cloruro de Sodio en Hydra vulgaris e Hydra viridissima

El aumento de la salinidad en los ecosistemas de agua dulce genera preocupación sobre los efectos adversos que puede provocar sobre las especies dulceacuícolas y el suelo. Las concentraciones óptimas de iones de sodio y calcio para que las especies del género Hydra tengan condiciones fisiológicas adecuadas son relativamente bajas. Este trabajo se enfocó en comparar la sensibilidad de H. vulgaris e H. viridissima frente al cloruro de sodio (NaCl). Las especies fueron mantenidas bajo condiciones de fotoperiodo, iluminación y temperatura controladas. Se obtuvieron las tasas de crecimiento y en ensayos de toxicidad aguda se calcularon las CE50 (Concentración efectiva media) y CL50 (Concentración letal media) como indicadores de la sensibilidad al NaCl. H. vulgaris fue más sensible al NaCl dado que la CL50-96h fue de 1,0 g/l NaCl (entre 0,8 - 1,2 g/l como intervalo de confianza al 95%), mientras que H. viridissima presentó una CL50-96h promedio de 2,6 g/l (entre 2,0 - 3,3 g/l como intervalo de confianza del 95%). Se considera que esta última fue más tolerante al NaCl, probablemente por la relación simbiótica con la microalga Chlorella vulgaris, ya que podría generar un efecto protector. Se espera que comprender el comportamiento de estos biomodelos respecto al aumento de la salinización permita la evaluación temprana de riesgos ecológicos en ecosistemas acuáticos tropicales.

The increase in salinity in freshwater ecosystems raises concern about the adverse effects that it can cause in freshwater species and the soil. The sodium and calcium ions optimum concentrations required for genus Hydra to have adequate physiological conditions are relatively low. This research compared the H. vulgaris and H. viridissima sodium chloride (NaCl) sensitivity. The species were propagated under photoperiod, lighting and temperature-controlled conditions. The growth rates were obtained and EC50 (mean effective concentration) and LC50 (mean lethal concentration) were calculated by means of acute toxicity tests as indicators of sensitivity to NaCl. For H. vulgaris the LC50-96h was 1.0 g/l of NaCl with a range between 0.8 - 1.2 g/l of NaCl, compared to H. viridissima presented an average LC50-96h of 2.6 g/l of NaCl with a 95% confidence interval between 2.0 - 3.3 g/l of NaCl. The H. viridissima was more tolerant, probably the symbiotic relationship with Chlorella vulgaris, can generate a protective effect. Understanding the behavior of these species with respect to increased salinization can allow an early assessment of ecological risks in tropical aquatic ecosystems.

Ecological and biological patterns of stream fish studies from the Piracicaba-CapivariJundiaí Basin (PCJ Basin, SP) assessed through a systematic review / Padrões ecológicos e biológicos de estudos de peixes de riachos da Bacia PiracicabaCapivari-Jundiaí (Bacia PCJ, SP) avaliados por meio de uma revisão sistemática

Tropical streams are among the most threatened ecosystems in the world. As such, studies carried out and compiled over spatial and temporal scales can provide useful information to examine patterns of species diversity and threats to their survival. Here we conducted a systematic review of published research on biological and ecological aspects of stream fish fauna found in the Piracicaba-Capivari-Jundiaí Basin, an industrial watershed of São Paulo State. We aimed to detect main patterns, trends and gaps in studies related to species composition, distribution, spatial and temporal scales, as well as in the covered topics. Results were related to main land uses, biomes and Conservation Units. A constant increase in published articles occurred from 2003 until 2016 with an average of 1.8 articles/year. Twenty-six publications were considered for the present study, reporting on fish samples obtained in 67 sites and resulting in 89 species. A high proportion of studies were concentrated in the Corumbataí sub-basin, and rarefaction curves indicated that stream fish richness in the PCJ Basin may be considerably higher than that shown by the actual numbers. Basin studies were unevenly distributed and did do not include such highly preserved areas as the Camanducaia, Jaguari and Jundiaí sub-basins. We emphasize the importance of further surveys in these regions, as well as in high priority conservation areas, which may lead to new insights for developing appropriate conservation strategies for this basin.

Riachos tropicais estão entre os ecossistemas mais ameaçados do mundo e a compilação de estudos temporais e espaciais pode fornecer informações úteis para examinar padrões de diversidade de espécies e ameaças nesses sistemas. Realizamos uma revisão sistemática das pesquisas publicadas sobre aspectos biológicos e ecológicos da ictiofauna de riachos da bacia do Piracicaba-Capivari-Jundiaí, uma bacia industrial do Estado de São Paulo. O objetivo foi detectar os principais padrões, tendências e lacunas em estudos relacionados à composição, distribuição de espécies, escalas espaciais, temporais e temas abordados. Os resultados foram relacionados aos principais usos do solo, biomas e Unidades de Conservação. Foi verificado um aumento constante de artigos entre 2003 e 2016, com média de 1,8 artigos/ano. Vinte e seis publicações foram consideradas para o estudo, que indicaram 67 locais amostrados, e o registro de 89 espécies. Uma alta proporção deles concentrou-se na sub-bacia de Corumbataí e curvas de rarefação indicaram que a riqueza de peixes de riacho na bacia do PCJ deve ser consideravelmente maior do que os números atuais. A distribuição desigual de estudos na bacia, que não inclui áreas altamente preservadas como as sub-bacias de Camanducaia, Jaguari e Jundiaí, enfatiza a necessidade de se obter mais informações nessas regiões, bem como em áreas de conservação de alta prioridade. Novas abordagens relacionadas a conceitos e teorias ecológicas em estudos futuros poderão fornecer informações que ajudem a desenvolver estratégias de conservação adequadas para esta bacia.

The introduction of Physa acuta (Gastropoda: Physidae) on Ilha Grande, Southeast Brazil, from initial stages to an established population / A introdução de Physa acuta Draparnaud, 1805 (Gastropoda: Physidae) na Ilha Grande, Sudeste do Brasil, desde os estágios iniciais até uma população estabelecida

This study presents a four-year follow-up of an introduced population of Physa acuta Draparnaud, 1805, from initial stages to an established population. This introduction occurred on a small impacted stream of Vila do Abraão, the main village of Ilha Grande (Angra dos Reis, Rio de Janeiro, Brazil). The population size increased during the study, and presented a relationship to environmental factors, especially with rainfall. On the initial stages of introduction prevailed the smaller specimens, but on the overall, predominated the intermediate size classes. After less than a year, P. acuta becomes established on this stream and was possibly affecting the other species found on the stream. The information presented here is useful to understand the invasion process of invasive snails, as well as directing conservation efforts.

Neste estudo é apresentado um acompanhamento de quatro anos de uma população de Physa acuta Draparnaud, 1805, desde os estágios iniciais da introdução até seu pleno estabelecimento. Esta introdução ocorreu em um pequeno riacho impactado da Vila do Abraão, o principal vilarejo da Ilha Grande (Angra dos Reis, Rio de Janeiro, Brasil). A população cresceu durante o estudo, e apresentou uma relação com os fatores ambientais, especialmente a pluviosidade. Nos estágios iniciais de introdução prevaleceram os exemplares menores, contudo no panorama geral, predominaram as classes de tamanho intermediárias. Em menos de um ano de introdução, P. acuta se estabeleceu neste riacho, e possivelmente está afetando as demais espécies encontradas no rio. A informação apresentada aqui é importante para a compreensão do processo de invasão de moluscos invasores no Brasil, assim como no direcionamento de esforços de conservação.

The introduction of Physa acuta (Gastropoda: Physidae) on Ilha Grande, Southeast Brazil, from initial stages to an established population

Abstract This study presents a four-year follow-up of an introduced population of Physa acuta Draparnaud, 1805, from initial stages to an established population. This introduction occurred on a small impacted stream of Vila do Abraão, the main village of Ilha Grande (Angra dos Reis, Rio de Janeiro, Brazil). The population size increased during the study, and presented a relationship to environmental factors, especially with rainfall. On the initial stages of introduction prevailed the smaller specimens, but on the overall, predominated the intermediate size classes. After less than a year, P. acuta becomes established on this stream and was possibly affecting the other species found on the stream. The information presented here is useful to understand the invasion process of invasive snails, as well as directing conservation efforts.

Resumo Neste estudo é apresentado um acompanhamento de quatro anos de uma população de Physa acuta Draparnaud, 1805, desde os estágios iniciais da introdução até seu pleno estabelecimento. Esta introdução ocorreu em um pequeno riacho impactado da Vila do Abraão, o principal vilarejo da Ilha Grande (Angra dos Reis, Rio de Janeiro, Brasil). A população cresceu durante o estudo, e apresentou uma relação com os fatores ambientais, especialmente a pluviosidade. Nos estágios iniciais de introdução prevaleceram os exemplares menores, contudo no panorama geral, predominaram as classes de tamanho intermediárias. Em menos de um ano de introdução, P. acuta se estabeleceu neste riacho, e possivelmente está afetando as demais espécies encontradas no rio. A informação apresentada aqui é importante para a compreensão do processo de invasão de moluscos invasores no Brasil, assim como no direcionamento de esforços de conservação.

Sequence analysis of Paragonimus internal transcribed spacer 2 and cyclooxygenase 1 genes in freshwater crabs in Henan Province / 中国血吸虫病防治杂志

Objective To investigate the sequences of internal transcribed spacer 2 (ITS2) and cyclooxygenase 1 (COX1) genes of Paragonimus metacercariae in freshwater crabs in Henan Province, identify the species of Paragonimus and evaluate its genetic relationships with Paragonimus isolates from other provinces in China. Methods Freshwater crabs were collected from 8 survey sites in Zhengzhou, Luoyang, Pingdingshan, Nanyang and Jiyuan cities of Henan Province from 2016 to 2021, and Paragonimus metacercariae were detected in freshwater crabs. Genomic DNA was extracted from Paragonimus metacercariae, and the ITS2 and COX1 genes were amplified using PCR assay, followed by sequencing of PCR amplification products. The gene sequences were spliced and aligned using the software DNASTAR, and aligned with the sequences of Paragonimus genes in the GenBank. Phylogenetic trees were created using the MEGA6 software with the Neighbor-Joining method based on ITS2 and COX1 gene sequences, with Fasciola hepatica as the outgroup. Results The detection rates of Paragonimus metacercariae were 6.83% (11/161), 50.82% (31/61), 18.52% (5/26), 8.76% (12/137), 14.29% (9/63), 17.76% (19/105), 18.50% (32/173) and 42.71% (41/96) in freshwater crabs from 8 survey sites in Zhengzhou, Luoyang, Pingdingshan, Nanyang and Jiyuan cities of Henan Province, with a mean detection rate of 19.46% (160/822), and a mean infection intensity of 0.57 metacercariae/g. The amplified ITS2 and COX1 gene fragments of Paragonimus were approximately 500 bp and 450 bp in lengths, respectively. The ITS2 gene sequences of Paragonimus metacercariae from 8 survey sites of Henan Province showed the highest homology (99.8% to 100.0%) with the gene sequence of P. skrjabini (GenBank accession number: MW960209.1), and phylogenetic analysis showed that the Paragonimus in this study was clustered into the same clade with P. skrjabini from Sichuan Province (GenBank accession number: AY618747.1), Guangxi Zhuang Autonomous Region (GenBank accession number: AY618729.1) and Hubei Province (GenBank accession number: AY618751.1), and P. miyazaki from Fujian Province (GenBank accession number: AY618741.1) and Japan (GenBank accession number: AB713405.1). The COX1 gene sequences of Paragonimus metacercariae from 8 survey sites of Henan Province showed the highest homology (90.0% to 100.0%) with the gene sequence of P. skrjabini (GenBank accession number: AY618798.1), and phylogenetic analysis showed that the Paragonimus in this study was clustered into the same clade with all P. skrjabini and clustered into the same sub-clade with P. skrjabini from Hubei Province (GenBank accession numbers: AY618782.1 and AY618764.1). Conclusions Paragonimus species from freshwater crabs in Henan Province were all characterized as P. skrjabini, and the ITS2 and COX1 gene sequences had the highest homology to those of P. skrjabini from Hubei Province. The results provide insights into study of Paragonimus in Henan Province and China.

Preliminary application of recombinase -aided amplification in detection of Clonorchis sinensis metacercariae in freshwater fish / 中国血吸虫病防治杂志

Objective To evaluate the performance of recombinase-aided amplification (RAA) assay in detection of Clonorchis sinensis metacercariae in freshwater fish samples, so as to provide insights into standardization and field application of this assay. Methods Wild freshwater fish samples were collected in the rivers of administrative villages where C. sinensis-infected residents lived in Jiangyan District, Xinghua County and Taixing County of Taizhou City, Jiangsu Province from June to September 2022. Genomic DNA was extracted from six freshwater fish specimens (5 g each) containing 0, 1, 2, 4, 8 and 16 C. sinensis metacercariae for fluorescent RAA assay, and the diagnostic sensitivity was evaluated. Fluorescent RAA assay was performed with genomic DNA from C. sinensis, Metorchis orientalis, Haplorchis pumilio and Centrocestus formosanus metacercariae as templates to evaluate its cross-reactions. In addition, the detection of fluorescent RAA assay and direct compression method for C. sinensis metacercariae was compared in field-collected freshwater fish samples. Results Positive amplification was found in fresh-water fish specimens containing different numbers of C. sinensis metacercariae, and fluorescent RAA assay was effective to detect one C. sinensis metacercaria in 5 g freshwater fish specimens within 20 min. Fluorescent RAA assay tested negative for DNA from M. orientalis, H. pumilio and C. formosanus metacercariae. Fluorescent RAA assay and direct compression method showed 5.36% (93/1 735) and 2.88% (50/1 735) detection rates for C. sinensis metacercariae in 1 735 field-collected freshwater fish samples, with a statistically significant difference seen (χ2 = 478.150, P < 0.001). There was a significant difference in the detection of C. sinensis metacercariae in different species of freshwater fish by both the direct compression method (χ2 = 11.20, P < 0.05) and fluorescent RAA assay (χ2 = 20.26, P < 0.001), and the detection of C. sinensis metacercariae was higher in Pseudorasbora parva than in other fish species by both the direct compression method and fluorescent RAA assay (both P values < 0.05). Conclusions Fluorescent RAA assay has a high sensitivity for detection of C. sinensis metacercariae in freshwater fish samples, and has no cross-reactions with M. orientalis, H. pumilio or C. formosanus metacercariae. Fluorescent RAA assay shows a higher accuracy for detection of C. sinensis infections in field-collected freshwater fish than the direct compression method.

Length-weight relationships of 24 stream-dwelling fish species from the Atlantic Forest in Rio de Janeiro, Brazil / Relação peso-comprimento de 24 espécies de peixes de riachos da Mata Atlântica do Rio de Janeiro, Brasil

Abstract The length-weight relationship parameter is important for obtaining fish weight and biomass data with relevant implications about species role on ecosystem functioning. Here we report the length-weight relationship (LWR) for 24 fish species from three streams located in the Atlantic Forest in the Rio de Janeiro State, Brazil. Fish were collected with electrofishing and standard length (cm) and wet weight (g) were measured to obtain the a and b parameters of the Log (W) = Log (a) + b Log (SL) equation. Length-weight relationships for seven out of 24 species (Hypostomus punctatus, Deuterodon taeniatus, Deuterodon hastatus, Deutorodon janeiroensis, Characidium vidali, Characidium interruptum and Rineloricaria zawadiskii) are reported for the first time. The length-weight relationships reported here contribute to the database that can support fish diversity conservation, fisheries management plans and studies on fish biology.

Resumo A relação peso-comprimento é um parâmetro importante para se obter o peso do peixe e sua biomassa, fornecendo dados importantes a serem incorporados em estudos sobre o papel das espécies em processos ecossistêmicos. Aqui, reportamos as relações peso-comprimento de 24 espécies de peixes de três rios localizados na Mata Atlântica do estado do Rio de Janeiro, Brasil. Os peixes foram coletados com a pesca elétrica e seu comprimento padrão (cm) e peso (g) foram medidos para obter os parâmetros a e b da equação Log(P) = Log (a) + b Log (CP). As relações peso-comprimento de sete entre as 24 espécies (Hypostomus punctatus, Deuterodon taeniatus, Deuterodon hastatus, Deutorodon janeiroensis, Characidium vidali, Characidium interruptum e Rineloricaria zawadiskii) são reportados pela primeira vez. As relações peso-comprimento informadas contribuem para a base de dados que auxilia na conservação da diversidade de peixes, na preparação de planos de manejo de pesca e estudos da biologia de peixes.

EphemBrazil: a curated online database and dashboard to explore the distribution of mayflies (Insecta: Ephemeroptera) from Brazil

ABSTRACT Despite the growing number of studies on mayflies (Ephemeroptera), especially in terms of description of new species and distributional records, the knowledge generated remains scattered across specialized literature. This dispersion poses a challenge for non-specialists, such as ecologists, hindering their ability to promote studies and conservation actions. In order to fill this gap, we are launching EphemBrazil: an online database focusing on the distribution of mayflies in Brazil. The main objectives of this platform are to compile a dataset of distributional information for the valid species of Ephemeroptera in Brazil, provide open access to this dataset through an interactive and easily updated website, and offer dynamic distributional maps for species, genera, and families of Brazilian mayflies. EphemBrazil aims to facilitate the retrieval and access of distributional and basic taxonomic information for the order.

An updated distribution list of Leptophlebiidae Banks, 1900 (Ephemeroptera: Insecta) for Piauí state / Uma lista de distribuição atualizada de Leptophlebiidae Banks, 1900 (Ephemeroptera: Insecta) para o estado do Piauí

Abstract Leptophlebiidae is the second most diverse family within Ephemeroptera, with species distributed across various States in Brazil, but with gaps in distribution records in others. Currently, nine species of Leptophlebiidae are recorded for the state of Piauí. Based on this information gap, the objective of this study was to present an updated species list of the family Leptophlebiidae new occurrence records and distributional sites for the state of Piauí, Brazil. By analyzing 447 specimens, we have significantly expanded our knowledge about the distribution of Leptophlebiidae species in the state of Piauí, increasing the recorded species from nine to 17. We have also added new occurrence records for six species and, for the first time, documented the presence of four genera. It is important to highlight that there is still an extensive area within the Cerrado and Brazilian semiarid regions where the occurrence of Ephemeroptera is unknown, confirming that the diversity in this area is underestimated and that knowledge of Ephemeroptera species and their distributions can expand with increased sampling efforts in the coming years, reducing the Linnean and Wallacean shortfall regarding this group. Our results also demonstrate the urgent need for inventories in the southern part of the state of Piauí, particularly in the sub-basins of the middle and upper Parnaíba river, which are considered suitable for monoculture expansion in Brazil.

Resumo Leptophlebiidae é a segunda família mais diversa de Ephemeroptera, com espécies distribuídas em vários estados do Brasil, mas com lacunas no registro de distribuição em outros. Por exemplo, no estado do Piauí tem registrado apenas nove espécies de Leptophlebiidae. Baseada nessa lacuna de informação, o objetivo deste estudo foi apresentar uma lista atualizada de espécies da família Leptophlebiidae, novos registros de ocorrência e sítios de distribuição para o estado do Piauí, Brasil. Ao analisar 447 exemplares, ampliamos significativamente nosso conhecimento sobre a distribuição das espécies de Leptophlebiidae no estado do Piauí, aumentando o número de espécies registradas de nove para 17. Também adicionamos novos registros de ocorrência para seis espécies e, pela primeira vez, documentamos a presença de quatro gêneros. Destacamos que ainda existe uma extensa área do Cerrado e semiárido brasileiro que se desconhece a ocorrência de Ephemeroptera, confirmando que a diversidade nessa área é subestimada e que o conhecimento sobre as espécies de Ephemeroptera e suas distribuições podem se expandir com o aumento do esforço amostral nos próximos anos, diminuindo as lacunas Lineana e Wallaceana sobre esse grupo. Nossos resultados também demonstram a necessidade emergencial de inventários no sul do Estado do Piauí, principalmente nas sub-bacias do médio e alto rio Parnaíba que é considerado adequado para a expansão da monocultura no Brasil.

Considering the role of the assassin snail Anentome helena as a biological control of Bithynia siamensis goniomphalos, the first intermediate host of Opisthorchis viverrini

This study aims to investigate whether the assassin snail Anentome helena may serve as a biological control agent of Bithynia siamensis goniomphalos, the 1st intermediate host of Opisthorchis viverrini. Experiments were carried out in the laboratory, and the results found that A. helena showed the variation and selection of mollusc prey. A. helena can consume B. siamensis goniomphalos, which is remarkable because this snail can compete with other snails and could be used as a biological control. The consumption rate of the predator was compared, and it was found that A. helena prefers to consume Indoplanorbis exustus, followed by Pomacea canaliculata, Melanoides tuberculata, Filopaludina sumatrensis speciosa, Lymnaea sp., and B. siamensis goniomphalos. This is the first report of an experimental study controlling B. siamensis goniomphalos using A. helena. Our data imply that A. helena can control the B. siamensis goniomphalos population with good results, especially in the absence of other snail species.

The introduction of Physa acuta (Gastropoda: Physidae) on Ilha Grande, Southeast Brazil, from initial stages to an established population / A introdução de Physa acuta Draparnaud, 1805 (Gastropoda: Physidae) na Ilha Grande, Sudeste do Brasil, desde os estágios iniciais até uma população estabelecida

Abstract This study presents a four-year follow-up of an introduced population of Physa acuta Draparnaud, 1805, from initial stages to an established population. This introduction occurred on a small impacted stream of Vila do Abraão, the main village of Ilha Grande (Angra dos Reis, Rio de Janeiro, Brazil). The population size increased during the study, and presented a relationship to environmental factors, especially with rainfall. On the initial stages of introduction prevailed the smaller specimens, but on the overall, predominated the intermediate size classes. After less than a year, P. acuta becomes established on this stream and was possibly affecting the other species found on the stream. The information presented here is useful to understand the invasion process of invasive snails, as well as directing conservation efforts.

Resumo Neste estudo é apresentado um acompanhamento de quatro anos de uma população de Physa acuta Draparnaud, 1805, desde os estágios iniciais da introdução até seu pleno estabelecimento. Esta introdução ocorreu em um pequeno riacho impactado da Vila do Abraão, o principal vilarejo da Ilha Grande (Angra dos Reis, Rio de Janeiro, Brasil). A população cresceu durante o estudo, e apresentou uma relação com os fatores ambientais, especialmente a pluviosidade. Nos estágios iniciais de introdução prevaleceram os exemplares menores, contudo no panorama geral, predominaram as classes de tamanho intermediárias. Em menos de um ano de introdução, P. acuta se estabeleceu neste riacho, e possivelmente está afetando as demais espécies encontradas no rio. A informação apresentada aqui é importante para a compreensão do processo de invasão de moluscos invasores no Brasil, assim como no direcionamento de esforços de conservação.

Which variables influence the herbivory amount on Montrichardia spp. (Araceae) in aquatic ecosystems? / ¿Qué variables influyen en la herbivoría en Montrichardia spp. (Araceae) en los ecosistemas acuáticos?

Abstract Introduction: The study of herbivory is fundamental in ecology and includes how plants invest in strategies and mechanisms to reduce herbivore damage. However, there is still a lack of information about how the environment, plant density, and functional traits influence herbivory in aquatic ecosystems. Objective: To assess if there is a relationship between herbivory, environmental variables, and plant traits two species of Montrichardia, a neotropical aquatic plant. Methods: In September 2018, we studied 78 specimens of Montrichardia arborescens and 18 of Montrichardia linifera, in 18 sites in Melgaço, Pará, Brazil. On each site, we measured water depth, distance to the margin, and plant density. From plants, we measured plant height and leaf thickness, and photographed the leaves to calculate the specific leaf area and percentage herbivory. To identify anatomical structures, we collected fully expanded leaves from three individuals per quadrat. Results: For M. arborescens, plants with thicker leaves and higher specific leaf area have less herbivore damage. For M. linifera, plants from deeper sites and with thicker leaves had more herbivore damage, while plants that grew farther from the margin had less damage. We found anatomical structures related to defense, such as idioblast cells with phenolic compounds, and cells with solid inclusions that can contribute to avoiding severe damage. Conclusions: Herbivory in these Montrichardia species can be explained by a combination of plant and environmental traits (patch isolation and water depth). The main plant traits are leaf thickness and area, but chemical compounds and solid inclusions also help Montrichardia to sustain less damage than other macrophytes.

Resumen Introducción: La herbivoría es fundamental para comprender cómo las plantas invierten en diferentes estrategias para evitar la depredación, lo que implica diferentes mecanismos de defensa. Factores relacionados con el medio ambiente, la densidad de plantas y/o los rasgos funcionales de las plantas pueden influir en la herbivoría en los ecosistemas acuáticos. Sin embargo, todavía falta información sobre cómo esos factores influyen en la herbivoría en los ecosistemas acuáticos y pueden contribuir a la carga de herbivoría. Objetivo: Evaluar si existe una relación entre la herbivoría y las variables ambientales (p. ej., profundidad del agua y distancia al margen), los factores ecológicos (densidad de plantas) y los rasgos estructurales de las plantas (altura, grosor de la hoja y área foliar) e indicar estructuras anatómicas que actúen junto con los rasgos estructurales en el sistema de defensa de especies de Montrichardia. Métodos: Se evaluaron 96 individuos de Montrichardia spp. (78 de M. arborescens y 18 de M. linifera, en 18 sitios) recolectados en septiembre de 2018. En cada sitio, se midió la profundidad del agua, la distancia al margen y la densidad de plantas. De los individuos, medimos la altura de la planta, el grosor de la hoja y fotografiamos las hojas para calcular el área foliar específica y la cantidad de herbivoría (en porcentaje). Para identificar las estructuras anatómicas relacionadas con la defensa de las plantas, se recogió hojas completamente expandidas de tres individuos por cuadrante. Resultados: Para M. arborescens, las plantas con hojas más gruesas y mayor área foliar específica tienen menos daño por herbivoría. Para M. linifera, las plantas con hojas más gruesas y que habitan en sitios más profundos tienen más daño por herbívoros, mientras que las plantas más alejadas del margen tienen menos daño por herbívoros. Se encontró estructuras anatómicas relacionadas con la defensa, como células idioblásticas con compuestos fenólicos y células con inclusiones sólidas que pueden contribuir a evitar daños severos en las características de las hojas. Conclusiones: Nuestros resultados indican que la herbivoría en las especies de Montrichardia podría explicarse por una combinación de características ambientales (aislamiento del parche y profundidad del agua) y de la planta. Descubrimos que los rasgos de las hojas eran factores importantes que impulsaban los cambios en la carga de herbivoría, especialmente el grosor de las hojas y el área foliar específica. Además, las especies de Montrichardia invierten en compuestos químicos e inclusiones sólidas para evitar daños graves en las hojas y, por lo tanto, pueden sufrir menos daños que otras especies de macrófitos.

Lista de especies de peces de la cuenca del río Ucayali, Perú

En este trabajo se presenta una lista actualizada de los registros de especies de peces de la cuenca del río Ucayali, Perú, provenientes de datos publicados y no publicados. Los resultados muestran que la cuenca del Ucayali presenta una ictiofauna rica y diversificada con el registro de 734 especies distribuidas en 15 órdenes, 49 familias y 292 géneros. La Ictiofauna está compuesta por peces miniatura (4%), peces pequeños (39%), medianos (41%), grandes (13%) y gigantes (3%). Los grupos dominantes son los Characiformes (312 especies, 43.0 %), Siluriformes (270 especies, 36.8%), Gymnotiformes (51 especies, 6.9%), y Cichliformes (50 especies, 6.8%). Parte de la ictiofauna es compartida con cuencas adyacentes como Marañón, y Amazonas peruano, principalmente en la región de confluencia, lo que contribuye a la notable diversidad de peces en la cuenca. Se observó una alta riqueza de especies con distribución restricta de los géneros Orestias, Astroblepus, Trichomycterus, Hemibrycon, entre otras especies, que ocurren en la región de cabeceras. Estimativos de riqueza de especies considerando su distribución por cada 100 m de altitud, indican que la cuenca del Ucayali esta subestimada, esperándose encontrar hasta 1125 especies. Los resultados aquí presentados son antecedentes que coadyuvaran a la toma de decisiones con fines de conservación y desarrollo sustentable en la región amazónica.

This study presents an updated checklists of the fish species recorded in the Ucayali River basin, Peru, from published and unpublished data. Ucayali River basin shows a rich and diversified ichthyofauna, with 734 species distributed in 15 orders, 49 families and 292 genera. It is composed by miniature fishes (4 %, < 2.6 cm of standard length), small fishes (39 %), medium fishes (41 %), large fishes (13 %) and giants (3 %). The dominant groups are Characiformes (312 species, 43.0 %), Siluriformes (270 species, 36.8 %), Gymnotiformes (51 species, 6.9 %) and Cichliformes (50 species, 6.8 %). Part of the ichthyofauna is shared with the adjacent basins as Marañón and Amazon, mainly in the confluence area, which contribute to the highest diversity of fishes in the Ucayali basin. High diversity of fishes with restricted distribution was observed, among them Orestias, Astroblepus, Trichomycterus, Hemibrycon genera that occur in the headwaters. Estimates of species richness considering their distribution per 100 m of altitude, show that the Ucayali basin is underestimated, expecting to find up to 1125 species. The results presented here are background information that will contribute to decision-making for conservation and sustainable development purposes in the Amazon region.

Digestibilidad aparente de materia seca, proteína y carbohidratos de la dieta con ensilado biológico de harina de maíz en Cryphiops (C.) caementarius / Apparent digestibility of dry matter, protein and carbohydrate of the diet with biological corn meal silage in Cryphiops (C.) caementarius

Resumen El objetivo del estudio fue determinar la digestibilidad aparente de la materia seca, proteína y carbohidratos de la dieta con ensilado biológico de harina de maíz en Cryphiops (C.) caementarius. Camarones adultos (4.50 a 7.20 cm de longitud total), se colectaron del río Pativilca (Lima, Perú) y en laboratorio se mantuvieron en recipientes individuales instalados dentro de seis acuarios (cuatro camarones por acuario). La dieta control fue sin ensilado y para la dieta experimental se reemplazó el 75% de la harina de maíz por el ensilado biológico de harina de maíz; ambas dietas tuvieron 1% de óxido crómico como marcador inerte. Los camarones fueron alimentados dos veces por día (40% a las 08:00 h y 60% a las 18:00 h) con el 6% del peso húmedo, durante 10 días. Para la colección de heces, los camarones fueron trasladados a un sistema de caja cribada (12 compartimentos) instalada dentro de un acuario. La digestibilidad aparente se determinó en base seca. Los camarones alimentados con la dieta con ensilado biológico de harina de maíz tuvieron altos coeficientes de digestibilidad aparente de carbohidratos (95.69%), proteína cruda (80.02%) y materia seca (76.19%), que aquellos alimentados con la dieta sin ensilado (p<0.05) que fue de 93.48, 78.21 y 74.36%, respectivamente. Los camarones adultos digieren con eficiencia los carbohidratos de la dieta con ensilado biológico de harina de maíz, en vez de la proteína de dicha dieta.

Abstract The aim of this study was to determine the apparent digestibility of dry matter, protein and carbohydrates of the diet with biological corn meal silage in Cryphiops (C.) caementarius. Adult prawn (4.50 to 7.20 cm total length) was collected from the Pativilca river (Lima, Peru) and in the laboratory each shrimp was kept in individual containers set up in six aquaria (four prawn per aquarium). The experimental diet was supplemented with corn meal biological silage and the control diet was without silage; both diets had 1% chromic oxide. Each diet had three replicates. Prawn was fed twice a day (40% at 08:00 h and 60% at 18:00 h) at 6% of wet weight for 10 days. For feces collection, the prawn was transferred to a system of sieved boxes (12 compartments) installed inside an aquarium. Apparent digestibility was determined on a dry basis. Prawn fed the diet with corn meal biological silage had higher apparent digestibility coefficient for carbohydrates (95.69%), crude protein (80.02%) and dry matter (76.19%) than those fed the diet without silage (p<0.05), which was 93.48, 78.21 and 74.36%, respectively. The adult prawn digested efficiently the carbohydrates of the corn meal biological silage diet, instead of the protein of this diet.

Prevalencia de Fasciola hepatica y Paramphistomidae en bovinos de doble propósito en una hacienda del trópico bajo andino colombiano / Prevalence of Fasciola hepatica and Paramphistomidae in dual purpose cattle in a farm in the Colombian low Andean tropic

RESUMEN En muchos países la fasciolosis y la paramfistomosis representan un grave problema para la salud del sector pecuario. En Colombia hay registros de ambas distomatosis en bovinos, la mayoría en el trópico alto andino, las cuales generan pérdidas econômicas anuales cercanas a 40 000 000 000 COP. El objetivo de esta investigación fue determinar la prevalencia de fasciolosis y paramfistomosis en vacunos de la hacienda La Candelaria, Caucasia (Colombia), y la presencia de caracoles hospederos intermediarios. Para cumplirlo, se realizó un estudio descriptivo de corte transversal con muestreo no probabilístico por conveniencia. Se recolectaron heces de los animales y se hizo el diagnóstico mediante la técnica modificada de Dennis. Se establecieron las prevalencias de los digeneos de acuerdo con el sexo, peso, edad y raza. Se recolectaron caracoles dulciacuícolas en la zona estudiada y se identificaron por morfologia. Se analizaron 466 muestras fecales de 178 bovinos, de las razas Cebú (Bos indicus), BON (blanco orejinegro) y del cruce entre ellas. Se diagnosticaron F. hepatica y Paramphistomidae con prevalencias del 2,2% y 30,9%, respectivamente. En el 1,1% de los vacunos se diagnosticó coinfección. Ambas trematodosis prevalecieron en las hembras (p = 0,03). Se identificaron moluscos dulciacuícolas Ampullariidae, Physidae y Planorbidae sin estadios larvarios de digeneos. Se concluyó que los bovinos de doble propósito de la hacienda La Candelaria están expuestos a F. hepatica y Paramphistomidae, y, probablemente, se infectan en los predios de la hacienda. Paramphistomidae es más prevalente que F. hepatica, lo cual concuerda con lo descrito en algunos estudios realizados en hatos del trópico alto andino colombiano. Paramphistomidae se encontró en todos los grupos etarios.

ABSTRACT Fasciolosis and paramphistomosis are a major health problem for the livestock economy worldwide. In Colombia, both distomatosis are reported in cattle, particularly in high Andean tropics, with annual economic losses close to COP 40 billion. The goal of this study was to determine the prevalence of fasciolosis and paramphistomosis in cattle from La Candelaria farm, Caucasia (Colombia), and the presence of intermediate host snails. A descriptive cross-sectional study was carried out with non-probability convenience sampling. Stool samples were collected from the animals and the diagnosis was made using the modified Dennis technique. Digenea prevalence were determined according to sex, weight, age, and race. Freshwater snails were collected in the studied area and were identified by morphology. 400 and 66 fecal samples from 178 bovines of the Zebu (Bos indicus), BON (white-eared white) breeds and the cross between them were analyzed. Fasciola hepatica and Paramphistomidae were diagnosed with a prevalence of 2,2% and 30,9%, respectively. Coinfection was diagnosed in 1,1% of the cattle. Both trematodosis were most frequent in females (p = 0,03). Freshwater molluscs Ampullariidae, Physidae and Planorbidae without digenea larval stages were identified. It was concluded that dual-purpose cattle from La Candelaria farm are exposed to F. hepatica and Paramphistomidae and are probably infected on the farm grounds. Paramphistomidae was more prevalent than F. hepatica, which agrees with other studies in herds from the Colombian high Andean tropics. Paramphistomidae was found in all age groups.

Detection of bloodworm larvae (Diptera: Chironomidae) in the golden apple snail Pomacea canaliculata (Lamarck, 1819) (Gastropoda: Ampullariidae) in Metro Manila

Background@#Pomacea canaliculata (Lamarck, 1819) is an invasive freshwater snail in the Philippines that damages crops but is consumed as food. It is known to harbor parasites, some of which are pathogenic to humans. @*Objectives@#The objective of this study is to examine P. canaliculata individuals present in Metro Manila for parasite infection and identify the parasites and other organisms associated with the snail using molecular identification. @*Methodology@#P. canaliculata were collected from rivers and marketplaces in Metro Manila. Individuals were crushed and digested in Ash's digestive fluid and observed under a microscope. Collected parasites were subjected to DNA barcoding of the COI gene for putative identification. @*Results@#A total of 462 snails were gathered from 15 sites, eight of which were market areas that sourced the snails from outside Metro Manila. No known parasites were found. Two snails were found to contain insect larvae in the mantle (0.43% infection). The closest BLAST matches for the two insect larvae were the chironomid fly Nilodorum tainanus (91.0% identity) from a snail in Sucat, Muntinlupa, and another chironomid Parachironomus sp. (92.8% identity) found in a snail originally from Cavite and brought to Calumpang, Marikina. @*Conclusion@#This study is the first report of the presence of chironomids in Philippine P. canaliculata. This could have an impact on the allergenic status of these mollusks if consumed while containing these chironomids. The absence of infection of other medically important parasites is possibly due to the patchy distribution of the snails and few interactions with the definitive hosts of known parasites.

New records of the occurrence of Megaleporinus macrocephalus (Garavello & Britski, 1988 (Characiformes, Anostomidae) from the basins of the Itapecuru and Mearim rivers in Maranhão, Northeastern Brazil / Novos registros da ocorrência de Megaleporinus macrocephalus (Garavello & Britski, 1988) (Characiformes, Anostomidae) nas bacias dos rios Itapecuru e Mearim no Maranhão, Nordeste, Brasil

The "piaussu", Megaleporinus macrocephalus is an anostomatid fish species native to the basin of the Paraguay River, in the Pantanal biome of western Brazil. However, this species has now been recorded in a number of other drainages, including those of the upper Paraná, Uruguay, Jacuí, Doce, Mucuri, and Paraíba do Sulrivers. This study presents two new records of the occurrence of M. macrocephalus, in the basins of the Itapecuru and Mearim rivers in the state of Maranhão, in the Brazilian Northeast. The piaussu is a large-bodied fish of commercial interest that is widely raised on fish farms, and its occurrence in the Itapecuru and Mearim rivers is likely the result of individuals escaping from fish tanks when they overflow during the rainy season. Morphological analyses and sequences of the Cytochrome Oxidase Subunit I (COI) gene confirmed the taxonomic identification of the specimens as M. macrocephalus. The COI sequences were 99.66% similar to those of M. macrocephalus deposited in the BOLDSystems database. These records extend the known distribution of M. macrocephalus to the basins of the Itapecuru and Mearim rivers in the Brazilian Northeast, highlighting a new case of introduction of exotic fish species into Brazilian river basins.

Megaleporinus macrocephalus é uma espécie de peixe anostomatídeo nativa da bacia do rio Paraguai, no bioma Pantanal do oeste do Brasil. No entanto, essa espécie já foi registrada em várias outras drenagens, incluindo as dos rios Alto Paraná, Uruguai, Jacuí, Doce, Mucuri e Paraíba do Sul. Este estudo apresenta dois novos registros da ocorrência de M. macrocephalus, nas bacias dos rios Itapecuru e Mearim, no estado do Maranhão, no nordeste brasileiro. O piaussu é um peixe de grande porte, de interesse comercial, amplamente criado em pisciculturas, e sua ocorrência nos rios Itapecuru e Mearim é provavelmente o resultado de indivíduos que escapam dos tanques quando transbordam durante a estação chuvosa. Análises morfológicas e sequências do gene da subunidade I do citocromo oxidase (COI) confirmaram a identificação taxonômica dos espécimes como M. macrocephalus. As sequências de COI foram 99,66% semelhantes às de M. macrocephalus depositadas no banco de dados BOLDSystems. Esses registros estendem a conhecida distribuição de M. macrocephalus às bacias dos rios Itapecuru e Mearim, no nordeste brasileiro, destacando um novo caso de introdução de espécies exóticas de peixes nas bacias hidrográficas brasileiras.