RESUMEN
A reverse-phase high pressure liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) has been developed for the quantitative analysis of hupehenine in the total alkaloids from Fritillaria hupehensis. Samples were analyzed on a reverse-phase column (Hypersil C-18) with a mobile phase of methanol:water:chloroform: triethylamine (85:15:1:0.6). The ELSD was set at the drift tube temperature of 68.3℃ and gas flow rate of 1.8 L/min. Hupehenine's retention time was 13.7 min with an asymmetry factor of 1.2. The validity of the method has been verified with linearity, limit of detection, accuracy and precision. The logarithmic linear curve was obtained from 8.936 to 134.04 μg/mL (r=0.9993). The detection limit (S/N＞3) of hupehenine was 1.79 μg/mL on the column. Intra-day RSD was 1.42% and inter-day RSD was 2.26% (3 days within a week). The average recovery of hupehenine was 101.50%, and RSD was 1.62%.
RESUMEN
OBJECTIVE:To study the microscopic TLC characteristics of Fritillaria thunbergii,Bulbus Fritillariae Cirrhosae,Fritillaria hupehensis,Fritillaria ussuriensis and Bulbus Fritillariae Pollidiflorae.METHODS: The five kinds of Fritillaria(powder)was identified microscopically in accordance with Chinese Pharmacopeia,and two development systems were applied for the TCL identification of the five kinds of Fritillaria.RESULTS: The microscopic characteristics of the 5 kinds of Fritillaria were obtained.Both peimine and peiminine were noted in the TLC of Fritillaria thanbergii,Bulbus Fritillariae Cirrhosae,Fritillaria hupehensis and Fritillaria ussuriensis,but were not noted in the TLC of Bulbus Fritillariae Pollidiflorae,however,imperialine was noted in the TLC of Bulbus Fritillariae Pollidiflorae.CONCLUSION: The results serve as basis for the identification of 5 kinds of Fritillaria.