RESUMEN
OBJECTIVE: To explore the new pattern of Chinese medicine solid preparations (CMSP) in vitro dissolution, a method testing the cell inhibition rate in combination with HPLC was used. METHODS: MTT assay was used to obtain the characteristic cell inhibition rate by Fufangbiejiaruangan tablet dissolving solutions at the pH 7.4 (phosphate buffer) dissolution medium at different times. From these results, the cumulative dissolution of Fufangbiejiaruangan tablets based on cell inhibition rate was obtained. The dissolution rate of paeoniflorin was determined by HPLC method. Then f2 similar factor method was used to evaluate the relevance of these two methods. RESULTS: f2 values was less than 50, indicating that there is a poor correlation between the two methods of measuring the dissolution rate. It is unfeasible to determine CMSP in vitro dissolution just by using paeoniflorin cumulative dissolution. CONCLUSION: Biological testing is expected to become CMSP in vitro dissolution of one of the evaluation tools.
RESUMEN
OBJECTIVE: To study the intestinal absorption characteristics of three components (paeoniflorin, phillyrin, ginsen-osides Rg1) of Fufangbiejiaruangan tablets and the effects of prescription on the intestinal absorption of paeoniflorin. METHODS: Everted intestinal sac method was adopted to mimic the intestinal absorption process of Fufangbiejiaruangan tablets extract at three concentrations. The intestinal absorption mechanisms of paeoniflorin, phillyrin and ginsenoside Rg1 were studied. HPLC assay was used to determine the three components and the time-accumulative absorption curves were drawn to compare the absorption characteristics of paeoniflorin in the prescription preparation and paeoniflorin solution. RESULTS: The absorption of the three components was linear at different intestinal segments and doses, and the correlation coefficients of the regression equations were all more than 0.9 (r≤0.9), which were consistent with zero order rate process. The Ka(s) of the components increased along with the increasing dosage, which indicated passive absorption. The cumulative absorption rate of paeoniflorin in Fufangbiejiaruangan tablets was better than one-component solution. CONCLUSION: The compatibility of Fufangbiejiaruangan tablets is beneficial to paeoniflorin absorption and ginsenoside Rg1 may be the indicator of the quality standard of Fufangbiejiaruangan tablets.