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Abstract The aim of this study was to recognize the mite fauna associated with apple orchards in southern Brazil and present a dichotomous key of the species sampled and those already reported in apple trees in southern Brazil. The studies were carried out in the 2020/2021 harvest in seven apple orchards of the Eva, Fuji and Gala cultivars located in the municipalities of Muitos Capões, Antônio Prado (Rio Grande do Sul state) and São Joaquim (Santa Catarina state). The orchards were divided into quadrants and sampling was carried out monthly. In each orchard, 40 plants were sampled, with three leaves of each plant collected in apical, median and basal regions of a median branch. In addition, monthly five species of spontaneous plants per orchard were sampled. A total of 8,425 mites were found, with the greatest abundance in Antônio Prado (50%), followed by Muitos Capões (35.5%) and São Joaquim (14.5%). The specimens found belong to 29 families, 64 genera and 99 species, in addition to mites of the order Oribatida. The greatest diversity was found in spontaneous vegetation (59 species), being 19 exclusives to apple trees and 21 species common to apple trees and spontaneous vegetation. Phytoseiidae was the family that presented the greatest richness, with 16 species, of which seven were common in apple trees and spontaneous plants. The most abundant species was Aculus schlechtendali Nalepa (Eriophyidae) (39.5%), followed by Panonychus ulmi (Koch) (Tetranychidae) (14.6%), Polyphagotarsonemus latus (Banks) (Tarsonemidae) (9.4%) and Neoseiulus californicus (McGregor) (Phytoseiidae) (7.4%). These results suggest that the mite fauna present in apple orchards in different landscapes and management are distinct, with a greater diversity of phytoseiid mites present in organic areas. Therefore, to maintain a greater diversity of predatory mites in these orchards, organic management seems to be the most appropriate. Furthermore, the high acarine diversity found in spontaneous vegetation demonstrates the importance of maintaining these plants in orchards that serve as refuges and reservoirs, favoring the permanence of natural enemies in these environments.
Resumo O objetivo deste estudo foi reconhecer a acarofauna associada a pomares de macieira no sul do Brasil e apresentar uma chave dicotômica das espécies amostradas e daquelas já relatadas em macieiras no sul do Brasil. Os estudos foram realizados na safra 2020/2021 em sete pomares de macieiras das cultivares Eva, Fuji e Gala localizados nos municípios de Muitos Capões, Antônio Prado (RS) e São Joaquim (SC). Os pomares foram divididos em quadrantes e a amostragem foi realizada mensalmente. Em cada pomar foram amostradas 40 plantas, sendo três folhas de cada planta coletadas nas regiões apical, mediana e basal de um ramo mediano. Além disso, mensalmente foram amostradas cinco espécies de vegetação espontânea por pomar. Foram encontrados 8.425 ácaros, com maior abundância em Antônio Prado (50%), seguido por Muitos Capões (35,5%) e São Joaquim (14,5%). Os exemplares encontrados pertencem a 29 famílias, 64 gêneros e 99 espécies, além de ácaros da ordem Oribatida. A maior abundância foi encontrada em vegetação espontânea (59 espécies), sendo 19 exclusivas de macieiras e 21 espécies comuns a macieiras e vegetação espontânea. Phytoseiidae foi a família que apresentou maior riqueza, com 16 espécies, das quais sete eram comuns em macieiras e vegetação espontânea. A espécie mais abundante foi Aculus schlechtendali Nalepa (Eriophyidae) (39,5%), seguida por Panonychus ulmi (Koch) (Tetranychidae) (14,6%), Polyphagotarsonemus latus (Banks) (Tarsonemidae) (9,4%) e Neoseiulus californicus (McGregor) (Phytoseiidae) (7,4%). Esses resultados sugerem que a acarofauna presente em pomares de macieira em diferentes paisagens e manejos são distintas, com maior diversidade de ácaros fitoseídeos presentes em áreas orgânicas. Portanto, para manter uma maior diversidade de ácaros predadores nesses pomares, o manejo orgânico parece ser o mais adequado. Além disso, a elevada diversidade de ácaros encontrada na vegetação espontânea demonstra a importância da manutenção destas plantas em pomares que servem como refúgios e reservatórios, favorecendo a permanência de inimigos naturais nestes ambientes.
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Objective:To analyze the supervision mode and problems of intensive countywide medical communities and explore the methods and strategies to improve their supervision system.Methods:From March 2022 to May 2022, a stratified random sampling method was used and the intensive countywide medical communities in the province were selected for field study. In-depth interviews were made with the staff of the healthcare administration departments and health insurance agencies affiliated with intensive countywide medical communities, directors of general hospitals, directors of township hospitals, and heads of functional departments. These interviews mainly covered the principal entities of countywide medical community supervision, supervision measures and contents, supervision effects, supervision weaknesses, and how to improve internal and external supervision mechanisms. The interviews results were analyzed using thematic framework analysis and the data were subject to a descriptive analysis.Results:23 persons of five intensive countywide medical communities were selected for key person interview. The finalized framework of regulatory elements of intensive countywide medical communities consists of such five general themes as regulatory subjects, regulatory content, regulatory measures, regulatory effectiveness and existing problems, comprising 14 level-1 indicators and 52 level-2 indicators. The main regulatory bodies were mainly government departments (15 times), the regulatory content was focused on medical resources (10 times), the regulatory measures cover specific regulations (4 times), specific activities (4 times) and assessment and evaluation (4 times). And the regulatory effectiveness demonstrated a currently strong regulation strength of medical communities (5 times), and the existing problems were insufficient government supervision (12 times), difficulties encountered by social forces in their supervision (11 times) and internal governance (5 times).Conclusions:The supervision system of intensive countywide medical communities should be optimized by improving the governmental supervision mechanism, inviting supervision of social forces and building an effective internal governance.
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Objective: Resin-modified glass ionomer (RMGI) cements are among the commonly used restorative materials in low stress-bearing areas and also for temporary restorations. The competition between acid-base reactions and light polymerization reactions in delayed curing of RMGIs can affect their physical and mechanical properties, as well as their degree of conversion. Since solubility, color stability, and opacity are among the main physical properties affecting the durability and clinical service of RMGI restorations, this study aimed to assess the effect of delayed curing on solubility, color stability, and opacity of Fuji II LC RMGI. Material and Methods: This in vitro, experimental study evaluated 80 Fuji II LC RMGI specimens (10 specimens per each in 4 groups) in terms of solubility, color stability, and opacity at 6 months later. Specimens were cured immediately or were cured with 1, 5 and 10 min delay. Results: Maximum solubility and minimum change in opacity and color stability at 6 months were noted in the group with delayed curing by 10 min. A significant difference was noted in the solubility of specimens cured after 10 min and 1 min. Significant differences were also noted in the opacity and color stability of specimens cured after 10 min and all other groups (P < 0.05). Conclusion: Delayed curing by 1 min decreased the solubility of RMGI specimens compared with immediate curing or curing after 5 min. Although this difference did not reach statistical significance. Color stability and changes in opacity are mainly influenced by the acid-base reactions rather than polymerization reactions.(AU)
Objetivo: Cimentos de ionômero de vidro modificado por resina (CIVMR) estão entre os materiais restauradores mais comumente utilizados em áreas de baixa tensão e também para restaurações temporárias. A competição entre reações ácido-base e reações provenientes da fotopolimerização tardia dos CIVRMs podem afetar suas propriedades físicas e mecânicas, bem como seu grau de conversão. Uma vez que a solubilidade, estabilidade de cor e opacidade estão entre as principais propriedades físicas que afetam a durabilidade e o tempo de serviço clinico de restaurações de CIVMR, este estudo teve como objetivo avaliar o efeito da fotopolimerizaçao tardia na solubilidade, estabilidade de cor e opacidade do CIVMR Fuji II LC. Material e Métodos: Este estudo experimental in vitro avaliou 80 espécimes de CIVMR Fuji II LC (4 grupos com 10 espécimes cada) em termos de solubilidade, estabilidade de cor e opacidade apos 6 meses. As amostras foram fotopolimerizadas imediatamente ou com 1, 5 e 10 min de atraso. Resultados: Máxima solubilidade e mínima alteração na opacidade e estabilidade da cor em 6 meses foram observadas no grupo com fotopolimerização tardia em 10 min. Uma diferença significativa foi observada na solubilidade das amostras fotopolimerizadas após 10 min e 1 min. Diferenças significativas também foram observadas na opacidade e estabilidade de cor das amostras fotopolimerizadas após 10 min e em todos os outros grupos (P <0,05). Conclusão: A fotopolimerizaçao tardia em 1 min diminuiu a solubilidade das amostras CIVMR em comparação com a fotopolimerizaçao imediata ou após 5 min. Embora essa diferença não tenha alcançado significância estatística. A estabilidade da cor e as mudanças na opacidade são influenciadas principalmente por reações ácido-base, em vez de reações causadas pela polimerização(AU)
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Solubilidad , Color , Cementos de Ionómero VítreoRESUMEN
The environmental gas concentration affects the storage period and quality of fruits and vegetables. High concentration CO₂ treating for a long time will cause damage to fruits, However, the specific molecular mechanism is unclear. To analyze the mechanism of CO₂ injury in apple, high-throughput sequencing technology of Illumina Hiseq 4000 and non-targeted metabolism technology were used to analyze the transcriptome sequencing and metabolomics analysis of browning flesh tissue of damage fruit and normal pulp tissue of the control group. A total of 6 332 differentially expressed genes were obtained, including 4 187 up-regulated genes and 2 145 down regulated genes. Functional analysis of the differentially expressed genes confirmed that the occurrence of CO₂ injury in apple was related to redox process, lipid metabolism, hormone signal transduction process and energy metabolism process. Twenty candidate browning genes were successfully screened, among which grxcr1 (md14g1137800) and gpx (md06g1081300) participated in the reactive oxygen species scavenging process, and pld1_ 2 (md15g1125000) and plcd (md07g1221900) participated in phospholipid acid synthesis and affected membrane metabolism. mdh1 (md05g1238800) participated in TCA cycle and affected energy metabolism. A total of 77 differential metabolites were obtained by metabolomic analysis, mainly organic acids, lipids, sugars and polyketones, including 35 metabolites related to browning. The metabolism of flavonoids was involved in the browning process of apple. Compared with the control tissue, the content of flavonoids such as catechin and quercetin decreased significantly in the damaged apple tissue, the antioxidant capacity of cells decreased, the redox state was unbalanced, and the cell structure was destroyed, resulting in browning. The results of this study further enrich the theoretical basis of CO₂ damage, and provide reference for the practical application of high concentration CO₂ preservation technology.
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Dióxido de Carbono , Frutas , Regulación de la Expresión Génica de las Plantas , Malus/genética , Metaboloma , TranscriptomaRESUMEN
ntroduction: Memory is a cognitive function essential to everyday life. Alcohol causes damage to the hippocampus. Music (especially Mozart music) has been reported to enhance memory function.Aim: This study investigated the possible role of different genres of Nigerian music (Afro-Hiphop and Fuji) on alcohol-induced hippocampal toxicity.Methodology: Thirty-six (36) Adult Wistar rats (105g-160g) were randomly distributed into 6 groups. Group A were administered 5ml/kg b.wt. of distilled water everyday for 28 days. Group B rats were administered 5ml/kg b.wt. of alcohol (20%) for 2 weeks. However for the first 2 weeks, the rats in Groups C and D were administered 5ml/kg b.wt. of alcohol (20%) and the rats in Groups E and F were exposed to Nigerian Fuji and hip hop music at 75dB-83dB respectively for 4 hours daily. For the last 2 weeks, the rats in C and D were exposed to Nigerian hip hop and fuji music respectively 75dB-83dB, for 4 hours daily and the rats in Group E and F were administered 5ml/kg b.wt of alcohol.Results: Higher memory capacity, oxidative status and normal histo-architecture of the hippocampus in group A rats were recorded. However, Group B rats showed a non-significant (p≥0.05) increase inbody weight, higher and lower memory capacity as shown by Morris water maze test and Y-Maze respectively, significant decrease (p≤0.05) in oxidative stress markers (SOD, CAT, GSH) and a significant (p≤0.05) increase in MDA and Acetylcholinesterase, structural distortion and neuronal degeneration as evident by a significant (p≤0.05) decrease in number of pyramidal cells when compared to the rats in groups A, C-F. The rats in group C-F had changes in their memory capacity at the 14th and 28th day.Conclusion: Music (AfroHip-Hop and Fuji) improved the histo-architecture of alcohol-distorted hippocampus in rats. However they do not have a definite effect on spatial memory.
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Introduction: Even with the advances and innovations inrestorative dentistry, microleakage is regarded one of the mainproblems in this area.Objective: To evaluate in vitro marginal microleakage ofBiodentine, Fuji II LC and SDR at the cervical level and at theinterface these materials with Tetric N Ceram Bulk fillcomposite and also to note the marginal microleakage whenthese materials were used as bulk fill materials.Materials and Methods: Cavities, standardized on the mesialand distal surfaces, were prepared in thirty molars andrandomly assigned to two groups (n = 30), according to themode of restoration done.Goup1 (control) in which testmaterials were used as bulk fill. These were further subdividedinto subgroups according to the material. SG1: Biodentine(SEPTODONT); SG2: Fuji II LC (GC); SG3: SDR (Dentsply).Group2 (experimental) in which test materials used as dentinsubstituent in class II sandwich technique followed byrestoration with Tetric N Ceram (Ivoclar vivadent). These werealso divided into subgroups, SG4: Biodentine + Tetric NCeram; SG5: Fuji II LC + Tetric N Ceram; SG6: SDR + Tetric NCeram. After storage for 24 hours in an incubator (37 °C), thesamples were submitted to the thermocycling test (500 cycles:5 °C/55 °C). They were later waterproofed, immersed in 1%methylene blue solution and sectioned in the mesialdistal direction for evaluation under stereomicroscope at 30xmagnification (Nikon SMZ 1500 Zoom Stereomicroscope)Scores from 0 to 3 using the ISO microleakage scoring system(ISO/TS 11405:2003). The ANOVA Test and Post- hoc Test,with a significance level of 5%, were used for statisticalanalysis.Conclusion: The study concluded that SDR (SD 2.38) showedthe least amount of microleakage both at the occlusal and atthe cervical levels in open sandwich restorations and alsowhen used as bulk fill materials as compared with Biodentine(SD 1.73) and Fuji II LC (SD 1.00).
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Objective To evaluate the effects of marginal adaptation with different materials on restoring dental wedge-shaped defects. Methods From January 2016 to July 2016,80 patients with 172 wedge -shaped defect teeth in Stomatological Hospital of Haishu District were chosen and randomly divided into three groups accord-ing to random number table method,26 patients with 55 defect teeth were treated with Fuji glass ionomer( group A) , 23 patients with 59 defect teeth were treated with Carey charismatic light-cured composite resin( group B) ,and the other 31 patients with 58 defect teeth were treated with beautifil flow plus reinforced polymer F03(group C). Marginal adaptation of three groups of different materials of the teeth were measured. Results The micro -leakage depth among the three groups had statistically significant difference(H=7. 036,P=0. 035). The micro-leakage depth of group A,B and C were (1. 10 ± 0. 05) mm,(0. 96 ± 0. 05) mm,(0. 78 ± 0. 06) mm,respectively. There was statisti-cally significant difference among the three groups(F=22. 371,P=0. 005). Conclusion The marginal adaptation of beautifil flow plus reinforced polymer F03 is superior to FujiⅡglass-ionome and Charisma composite resin,and has benefits for treatment of wedge-shaped defects.
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Objective To study the clinical application of Fuji intelligent color enhancement ( FICE) on the diagnosis of vocal leukoplakia and esophageal diseases. Methods A total of 50 patients suspected with vocal leukoplakia by electronic laryngoscopy were enrolled for undergoing meticulous examination of vocal cords and esophagus using FICE from January 2014 to January 2016. The diagnostic value of FICE for vocal leukoplakia were analyzed. Results Among the 50 patients, 50 lesions were found using laryngoscopy and 52 lesions were found using FICE. There were significant differences between FICE mode and white light mode on the capillary pattern score and lesion boundary resolution score ( all P<0. 05) . The diagnostic coincidence rate of FICE mode with pathology was higher than that of white light mode [ 89. 4％ ( 42/47 ) VS 68. 1％ ( 32/47 ) , P = 0. 01 ] . Among the 50 patients suspected with vocal leukoplakia, 31 combined with gastroesophageal reflux disease, and were given proton-pump inhibitor treatment. In the follow-up after three months, lesions of 16 cases alleviated, 13 cases disappeared, and 2 cases had no significant changes. Conclusion FICE has a higher clinical value than conventional laryngoscopy and white light endoscopy on the diagnosis of vocal leukoplakia.
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Objective To investigate the observation effect of Fuji intelligent chromoendoscopy (FICE) in central type lung cancer,to select the optimal wavelength combination and to explore its diagnostic value for central type bronchogenic lung carcinoma. Methods One hundred and forty-six cases of definite histopathological diagnosis by bronchoscopy examination with preserved intact data in the Chongqing Municipal Tumor Hospital from January 2016 to October 2016 were included into this study. The white light bronchoscopy(WLB) and FICE were used to conduct the observation respectively. The capillary morphology was respectively scored by using the 10 wave combinations in FICE. The best wave combination was selected for conducting the forceps biopsy. Finally the pathological results served as the gold standard for conducting the comparative study. Results The difference of lesion observation effect among 10 sets of FICE wave combination had statistical significance (P<0.01), the wave combination 8 [R= 540 (2), G= 505 (4), B= 420 (5), wavelegnth(nm gain value)] was superior to that of other wave combinations in observing lesion vascular texture (Rank% = 9.74). The coincidence rate of FICE for judging the lesion property was 88.4 %. The detection rate of FICE combined with WLB for central type lung cancer was 96.6 %, compared with that of single WLB, the detection rate had statistically significant difference (P<0.01). Conclusion FICE wave combination 8 [R= 540 (2) ,G=505 (4),B=420 (5) ,wavelength(nm, gain value)] is most ideal for observing superficial mucosal capillary vascular morphology of central type lung cancer lesion tissue.
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AIM: The purpose of this study was to evaluate the leakage of bleaching agents through 3 different bases into the obturated pulp space and dentinal tubules in intra coronal bleaching. MATERIAL AND METHODS: Fifty-five freshly extracted, intact non-carious single rooted teeth were collected and stored in formalin until used. The specimen divided into experimental and control group. Experimental group were further subdivided into 3 subgroups on the base of cervical base materials. Group I was Type II GIC, Group II was Fuji II LC and Group III was Hybrid composite. The control groups was divides into negative group (received the bleaching agent and received complete external coating including access cavity) and positive control group (did not receive any external coating). The obturated pulp space were removed at a level of 3 mm below the cemento enamel junction and filled with dry cotton pellets and temporized with temporary cement, stored at ± 37°C and 100% relative humidity for five days, the bleaching material were placed in the pulp chamber (a paste of sodium perborate i.e. 0.15g and 30% hydrogen peroxide 0.05ml), and then stored for 3 days in an incubator. Later on the teeth were immersed in India ink dye and stored at ± 37°C and 100% relative humidity for 5 days. Specimens were examined under binocular microscope with a stage micrometer to analyze the degree of dye penetration. RESULTS: The minimal leakage values were observed with type II glass ionomer cement followed by light cure glass ionomer cement and lastly by hybrid composite. CONCLUSION: The present invitro investigation evaluated the microleakage observed with different cervical base materials during non-vital bleaching procedures.
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Background: Occlusal surfaces of the molars are highly susceptible to dental decay. Pit and fissure sealants are one of the best methods of preventing fissure caries. The main problem encountered is microleakage leading to deterioration of material and increased possibility of the development of secondary caries. Aims: To assess and compare the marginal integrity of glass ionomer (Fuji VII) and resin based (Clinpro) fissure sealants using invasive and non-invasive technique. Setting and Design: Experimental in-vitro study. Materials and Methods: An in-vitro study was conducted on 40 healthy, extracted premolars. The teeth were divided into 4 groups. Group I: Clinpro-invasive technique, group II: Clinpro-non-invasive technique, group III: Fuji VII-invasive technique, group IV: Fuji VII-non-invasive technique. Sealants were applied and subjected to an evaluation under SEM (scanning electron microscopy) for the width of the marginal gap. ANOVA (analysis of variance) and Duncan's multiple range tests were used for the statistical analysis. Results: The mean width of the marginal gap was 0.4089 μm in group I as compared to 3.0485 μm in group III; and the difference was statistically highly significant (P<0.001). In non-invasive technique, Clinpro and Fuji VII showed a mean marginal gap width of 0.4486 μm and 3.0485 μm, respectively. This difference was statistically highly significant at a P<0.001. There was no statistically significant difference in the mean widths between invasive and non-invasive techniques. Conclusion: Clinpro performed better in terms of marginal adaptation than did the Fuji VII sealant. The techniques (Invasive and non-invasive) does not influence the marginal integrity significantly.
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Resinas Acrílicas/química , Resinas Compuestas/química , Fisuras Dentales/cirugía , Fisuras Dentales/terapia , Resinas Epoxi/química , Cementos de Ionómero Vítreo/química , Humanos , Selladores de Fosas y Fisuras/químicaRESUMEN
Objective To evaluate the flexible spectral imaging color enhancement (FICE) system in the diagnosis of early esophageal carcinoma and precancerous lesions.Methods A total of 257 patients with suspicious esophageal lesions were examined successively by FICE,magnifying FICE,iodine dyeing endoscopy and magnifying iodine dyeing endoscopy.Findings were compared with the pathologic diagnosis.Results The positive rates of early esophageal carcinoma by FICE (92.6％,25/27) and iodine dyeing endoscopy (88.9％,24/27) were not significantly different (P =0.642),nor were those of magnifying FICE (96.3％,26/27) and magnifying iodine dyeing endoscopy (92.6％,25/27),(P =0.556).The magnifying FICE could reveal the IPCL of early esophageal carcinoma clearly.Early esophageal carcinoma and advanced neoplasia were mainly type Ⅳ + Ⅴ,low-level neoplasia and esophagitis were type Ⅱ + Ⅲ,and normal esophagus was type Ⅰ.However,the magnifying iodine dyeing endoscopy was not able to reveal IPCL.There was no adverse reaction in FICE,but the adverse reaction rate was 12.8％ (33/257) in iodine dyeing endoscopy.Conclusion Magnifying FICE can accurately determine the pathological types of early esophageal carcinoma,which is an effective complement to iodine dyeing endoscopy.
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ObjectiveTo evaluate Fuji intelligent color enhancement (FICE) chromoendoscopy for diagnosis of early gastric cancer. MethodsFrom February 2010 to March 2011 ,a total of 67 patients with suspected gastric mucosal lesions were enrolled in this study. The lesions were observed with magnifying endoscopy, FICE, magnifying chromoendoscopy and indigo-carmine-magnifying-chromoendoscopy.Suspected gastric mucosal pit patterns and microvascular morphology were compared. Targeted biopsy was performed on suspected locations. Sensitivity, specificity and pathological consistency were compared between the 3 procedures. ResultsOut of 67 patients, 17 were diagnosed as having early gastric cancer.There was no difference among magnifying endoscopy, FICE magnifying chromoendoscopy and indigo-carmine-magnifying-chromoendoscopy in pit pattern findings,however, FICE magnifying chromoendoscopy was superior to other 2 procedures in displaying capillary loop. The sensibility, specificity and pathological accordance rate of FICE magnifying chromoendoscopy were 94. 1％ ( 16/17 ), 98. 0％ (49/50) and 97.0％(65/67), which were higher than those of the magnifying endoscopy [58.8％ ( 10/17), 84％ (42/50)and 77.6％ ( 52/67 )] ( P ＜ 0. 05 ), and were not different from those of indigo-carmine-magnifying-chromoendoscopy [88.2％ (15/17), 96％ (48/50) and 94.0％ (63/67)] (P ＞0.05). Conclusion FICE magnifying chromoendoscopy provides rather clear images of gastric mucosa and intrapapillary capillary, thereby improving the accuracy of endoscopic biopsy and then imporving the diagnosis rate of early gastric cancer.
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ObjectiveTo evaluate the Fuji intelligent chromo endoscopy (FICE) in the diagnosis of Barrett esophagus (BE).MethodsFrom September 2010 to March 2011,a total of 180 patients with suspected reflux esophagitis were examined successively by FICE,magnifying FICE,acetic dyeing endoscopy and magnifying acetic dyeing endoscopy.The diagnosis was made out under the observation of lesion extensions,superficial mucosa contrast ratio,pit patterns and capillary forms of BE.The endoscopic diagnosis was made and compared with the pathologic diagnosis,and the consistency of the diagnosis was evaluated by Kappa value.ResultsBE was confirmed in 35 patients ( 19.4％ ) pathologically.The consistency rates of diagnosis under FICE and acetic dyeing endoscopy were 81.7％ and 72.8％ ( P ＜ 0.05 ).The consistency rates of diagnosis under magnifying FICE and magnifying acetic dyeing endoscopy were 97.8％ and 85.6％,respectively (P ＜ 0.05).FICE magnifying endoscopy revealed better mucosal structures of capillaries than magnifying acetic dyeing endoscopy did ( P ＜ 0.05 ),but there was no significant difference in revealing of duct openings (P ＞ 0.05).The specificity,sensitivity,positive predictive value,negative predictive value and Kappa value of FICE in diagnosis of BE were 82.1％,80.0％,51.9％,94.4％ and 0.52,respectively,which were 73.2％,71.4％,39.1％,91.4％ and 0.34 of acetic dyeing endoscopy,98.6％,94.3％,94.3％,98.6％ and 0.93 of magnifying FICE,and 88.3％,74.3％,60.5％,93.4％ and 0.58 for magnifying acetic dyeing endoscopy.ConclusionAs a neotypical endoscopic system,magnifying FICE could exhibit clearly the pit patterns and microvascular structures of esophagus mucosa,and it can capture the optimal images of Barrett's epithelium.FICE could improve the diagnosis of BE in vivo.
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La enfermedad por reflujo gastroesofágico es una enfermedad crónica del esófago, su diagnóstico clínico está basado en sus síntomas, pero la endoscopia determina dos entidades clínicas. El 50 por ciento de los pacientes con enfermedad por reflujo gastroesofágico tienen enfermedad por reflujo gastroesofágico y esofagitis. El objetivo de este trabajo es demostrar que la endoscopia de alta resolución con magnificación y Fuji intelligent color enhancement, tienen un mayor porcentaje diagnóstico de enfermedad por reflujo gastroesofágico, tienen enfermedad por reflujo gastroesofágico y esofagitis cuando se compara con la endoscopia de alta resolución. Materiales y métodos: Se realizó endoscopia de alta resolución y endoscopia de alta resolución con magnificación y Fuji intelligent color enhancement a 50 pacientes con el diagnóstico clínico de enfermedad por reflujo gastroesofágico. Se comparó con un grupo control de 50 pacientes. Resultados: La endoscopia de alta resolución dio un 70 por ciento de diagnóstico de enfermedad por reflujo gastroesofágico tienen enfermedad por reflujo gastroesofágico y esofagitis y la endoscopia de alta resolución con magnificación y fuji intelligent color enhancement dio un 98 por ciento de diagnóstico de enfermedad por reflujo gastroesofágico tienen enfermedad por reflujo gastroesofágico y esofagitis (P=0,0002), estadísticamente significativa. Conclusión: La endoscopia de alta resolución con magnificación y fuji intelligent color enhancement, es el método endoscópico de elección, para diagnosticar la existencia de enfermedad por reflujo gastroesofágico tienen enfermedad por reflujo gastroesofágico y esofagitis, en los pacientes con diagnóstico clínico de enfermedad por reflujo gastroesofágico
The gastroesophageal reflux disease (GERD) is a chronic illness of the esophagus, where the clinical diagnosis is based on their symptoms, but the Endoscopy determines two clinical entities. 50 percent of the patients with gastroesophageal reflux disease has esophagitis. The Objective of this trial was demonstrate that the High Resolution Endoscopy with Magnification and Fuji Intelligent Color Enhancement (FICE) had a major percentage diagnosis of ERGEE, when it was compared with the High Resolution Endoscopy. Materials and Methods: It was performed High Resolution Endoscopy and High Resolution Endoscopy with Magnification and Fuji intelligent color enhancement to 50 patients with the clinical diagnosis of gastroesophageal reflux disease, and it was compared with a control group of 50 patients. Results: The high resolution endoscopy demonstrated 70 percent of diagnosis of gastroesophageal reflux disease has esophagitis and the high resolution endoscopy with magnification and Fuji intelligent color enhancement reported 98 percent of diagnosis of gastroesophageal reflux disease has esophagitis (P=0.0002), statistically significant. There was not significant statistical difference in the control group. Conclusion: The high resolution endoscopy with magnification and Fuji intelligent color enhancement was the method of choice to evaluate the existence of gastroesophageal reflux disease has esophagitis, in patients with clinical diagnosis of gastroesophageal reflux disease
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Humanos , Masculino , Femenino , Endoscopía Gastrointestinal/métodos , Esofagitis Péptica/diagnóstico , Esofagitis Péptica , Magnificación Radiográfica/métodos , Reflujo Gastroesofágico/diagnóstico , GastroenterologíaRESUMEN
Objective The aim of this study was to describe the FICE application on gastric mucosa characteristics including normal and pathological changes, with or without H.pylori infection, and its corre-lation with histopathoingical evidence. Methods A total of 32 patients with dyspepsia symptoms and 5 healthy controls were enrolled into study. Each one underwent esophngogastreduedenoscopy (EGD) examina-tion with FICE and magnified observation. The whole stomach was examined by 3 steps: including conven-tional endoscopy followed by magnifying and FICE observation of the gastric antrum and body as well as biop-sies. All the patients were asked to take the rapid urease test (RUT) 、13C -urea breath test (13C-UBT) . Gastric antrum and body were both sub-classified into following 3 patterns by FICE observation and high reso-lution magnifying endoscopy. The sensitivity, specificity of each FICE pattern of both gastric antrum and body were analysed for the assessment of H. pylori infection, and the consistency with the results of RUT and 13C-UBT. Furthermore, the histopathologic parameters including inflammation、activity、atrophy and intestinal metaplasia were also assessed, Results FICE patterns of gastric antrum and body of all 5 control subjects were type Ⅰ, corresponded to an H. pylori negative and non-atrephy gastric mueosa. In study group on gastric antrum, 14 cases of FICE type Ⅰ pattern were noted and only 1/14(7. 1%) corresponded to an H. pylori positive gastric mucosa. 13 cases of FICE type Ⅱ pattern were noted and 10/13 (76. 9%) corresponded to an H.pylori positive mucosa and 9/13(69. 2%) were positive for both gastric atrophy. 5 cases of FICE type Ⅲ pattern were noted and 5/5 (100%) corresponded to an H. pylori positive mucosa and 3/5 (60%) were positive for both gastric atrophy and intestinal metaplasia. There was statistical difference in prediction of H. pylori infection between type Ⅰ FICE pattern and type Ⅱ or type Ⅲ pattern on gastric antrum (P <0. 01). In study group on gastric body, 15 cases of FICE type Ⅰ pattern were noted and only 1/14(7. 1%) corre-sponded to an H. pylori positive gastric mucosa. 13 cases of FICE type Ⅱ pattern were noted and 11/13 (84. 6%) corresponded to an H. pylori positive mucosa. 4 cases of FICE type Ⅲ pattern were noted and 4/4 (100%) corresponded to an H.pylori positive mucosa. There was statistical difference in prediction of H. pylori infection between type Ⅰ FICE pattern and type Ⅱ or type Ⅲ pattern on gastric body (P < 0. 01). Conclusion FICE in combination with high resolution magnifying endoscopy is valuable for identifying the normal gastric mucosa, H.pylori infection and its associated gastritis, gastric atrophy as well as intestinal metaplasia.
RESUMEN
Objective To establish a method for determination of matrine in Fuji Lotion. Methods An Inertsil NH2 column (250 mm?4.6 mm, 5 ?m) was used with acetonitrile-ethanol absolute-3% phosphoric acid (83∶8.5∶8.5) as mobile phase, the flow rate was 1.0 mL/min, and the UV detection wavelength was set at 220 nm. Results The calibration curve of matrine was linear in range of 0.039～1.560 ?g, r=0.999 7. The average recovery was 97.8% with RSD of 1.1% (n=6). Conclusion The method is simple, quick and accurate. It can be used for the quality control of Fuji Lotion.