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This study was designed to evaluate the effect of germination time on the proximate composition, functional and pasting properties of flours from red and brown varieties of pigeon pea seeds. The red and brown pigeon pea seeds were cleaned and germinated for 48, 72 and 96 h, respectively and processed into flours, while the flours produced from the ungerminated red and brown pigeon pea seeds served as control samples. The flours obtained were analyzed for proximate, functional and pasting properties using standard methods. The proximate composition of the samples showed that the flours produced from the red pigeon pea seeds had a range of 6.24 � 9.34% moisture, 10.37 � 20.95% crude protein, 1.21 � 1.12% fat, 2.32 � 3.32% ash, 3.14 � 4.23% crude fibre, 76.75-61.07% carbohydrate and 329.29 � 338.10KJ/100g energy, while that of the brown pigeon pea flours had a range of 6.44-9.77% moisture, 10.47-23.69% crude protein, 1.23-1.13% fat, 2.42-3.13% ash, 3.18-4.31% crude fibre, 76.26-57.99% carbohydrate and 358.18-336.83KJ/100g energy. The functional properties of the red pigeon pea flours revealed that the bulk density, water absorption capacity, oil absorption capacity, swelling capacity, gelation capacity and foam capacity ranged from 0.58-022 ml/g, 1.36-1.58 %, 1.40-1.61 %, 2.13-2.38 ml/g, 8.24-11.13 ml/g and 12.28-12.01 %, while that of the brown variety ranged from 0.63-0.36 ml/g, 1.47-1.69 %, 1.46-1.67 %, 1.67-2.46 ml/g, 8.88-11.41 ml/g and 13.33-13.06 %, respectively. The pasting properties of the red pigeon pea flours also showed that the peak, trough, breakdown, set back and final viscosities as well as the peak time and pasting temperature ranged from 3.10 � 5.556cP, 3.35 � 4.86cP, 2.65 � 4.57cP, 5.23 � 7.75cP, 8.82 -10.02cP, 6.79 � 7.34min and 69.79 � 92.710C, while that of the brown variety ranged from 3.59 � 6.21cP, 4.19 � 5.61cP, 3.77 � 5.23cP, 5.59 � 8.25cP, 9.60 � 11.08cP, 7.07 � 7.99min and 80.11 � 99.21oC, respectively. The study, however, revealed that the flours produced from both the red and brown pigeon pea seeds could be generally used as nutrient dense and functional ingredients in the preparation of a wide range of food products especially in Nigeria and other developing countries of the world where there is acute shortage of protein in order to ensure food and nutritional security.
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An experiment was conducted to evaluate the cold tolerance ability of ten boro rice genotypes in the laboratory conditions for suitability of growing under low temperature stress. The experiment was laid out in Factorial CRD and the low temperature regimes was provided under controlled environment (Walk-in cold chamber by Saveer Biotech Limited) for germination studies that could maintain the specific low temperature. The experiment was conducted in the Department of Crop Physiology and Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam, India. The germination tests were conducted under four different temperature regimes where seed germination under ambient (T4=28 ± 0.2oC) was considered as control. Low-temperature treatments were provided by the growth chamber at three different levels of low temperature viz. (T3=10-12°C), (T2=7-9oC) and (T1=5-6°C). Per cent germination, germination index, shoot length, length of longest root and seed vigour index were considered for the study. The germination percent, germination index, shoot length, length of longest root and seed vigour index was recorded highest under ambient condition (T4) irrespective of all the genotypes. Seeds grown under T3 showed considerable germination values only after 28 days of germination studies, whereas T1 and T2 showed no germination. The study revealed that amongst all the ten genotypes, Kanaklata and Sona Mahsuri revealed superior performance in germination percentage, germination index, shoot length, length of the longest root and seed vigour index. In the correlation studies, germination % was significantly correlated with germination index, shoot length, length of the longest and SVI at 5% probability level. GI was also positively and significantly correlated with Shoot length (SL), Length of longest root (LLR) and Seed Vigour Index (SVI). The present study revealed that Sona Mahsuri and Kanaklata was found to be the ideal genotypes for germination under low temperature (10-12oC). Therefore, Farmers growing boro rice under low temperature regimes could take the opportunity of selecting the varieties viz. Kanaklata and Sona Mahsuri for higher yield.
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Considering ten (10) Lentil genotypes, some vibrant physiological activities of seed traits and germination linked enzymes was observed for assessing the status on heritability. The study on two successive years showed significant hierarchy for some seed traits in 1st year related to seedling performances and biomolecular activities though others characters showed non-significant association. Considering two years, the genotypes showed significant demarcation among them where the maximum value was in ASHA for all seed traits with enzyme activity. The interacted values of genotypes x year showed significant variation for most of the seed traits excepting seedling length and seedling dry weight. The study on genetic analysis indicated minimum deviation in between genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) that recognized the genetic influence of all considerable seed traits. High heritability (H2%) along with genetic advance indicated the impact of additive gene for all characters except soluble protein and percent of germination. In correlation matrix, the non-significant or negative relationship was followed in between germination linked biomolecules and physiological performances of seed though positive significant relation was followed within each group. Therefore, the selected parameters may be considered in breeding schedule for quality seed production of Lentil.
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Horsegram (Macrotyloma uniflorum) is a robust, drought-resistant crop that is valued for its high protein content and is a significant food source in many parts of the world. Horsegram seeds must be stored properly to preserve their freshness, nutritional value, and viability for future planting. In this article an investigation was carried out to evaluate the suitable seed treatment and packaging materials for maintaining the shelf life of horsegram seeds during storage. The laboratory experiment was carried out at Department of Seed Science and Technology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, India. The graded seeds of horsegram were treated with different botanicals (Neem seed kernel powder @ 5g/kg & Pungam leaf powder @ 5g/kg), herbal oil (Neem oil @ 5ml/kg) and chemical (Malathion @ 5g/kg) and packed in cloth and super grain bags which was kept under ambient condition. The physical, physiological and biochemical parameters were assessed from the stored seed samples. Horsegram seeds dusted with malathion at 5 g/kg and then packed in a super grain bag have better germination (94%), root length (18.33 cm), shoot length (9.10 cm), DMP (0.165 g seedlings-10), vigour index I and II (2551 and 16.2), and also biochemical parameters like the lowest electrical conductivity (0.121 dSm-1), highest enzyme activity like dehydrogenase (0.521) and catalase (0.213 units g-1) and protein level (19.21%) was also high in malathion (5 g/kg) treated seeds packed in a super grain bag after storage for 8 months.
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Sesamum is an important oilseed crop affected by toxigenic fungal pathogens viz., Alternaria, Macrophomina, Fusarium and Aspergillus spp. Among these, A. alternata and M. phaseolina are seed borne pathogens causing yield loss to an extent of 20 to 40 per cent. The effect of A. alternata and M. phaseolina on seed quality parameters of susceptible cv. Swetha was investigated during 2022-2023. The A. alternata and M. phaseolina inoculated and uninoculated seeds of both test pathogens were tested for germination and seedling growth by rolled paper method and pot culture studies. Significant differences in seed germination, seedling length, seedling dry mass, seedling vigour index (SVI) - I and II and seed moisture content were observed in susceptible cv. Swetha. The results indicated that A. alternata recorded lower seed germination percentage (60.50%), seedling length (6.56 cm), dry mass (9.12 mg) and SVI-I and II (656 and 552 respectively) over untreated seeds recording high germination (82.25%), seedling length (14.84 cm), dry mass (13.17 mg) and SVI-I and II (1221 and 1116 respectively). Similar results were observed in M. phaseolina treated seeds where the pathogen treated seeds recorded lower seed germination percentage (70.75%), seedling length (9.42 cm), dry mass (8.71 mg) and SVI-I and II (667 and 599 respectively) than pathogen untreated seeds recording high seed germination (82.50%), seedling length (14.25 cm), dry mass (13.30 mg) and SVI-I and II (1175 and 1097 respectively).
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A field experiment was conducted in 2020–21 at the Fruit Research Station, Imaliya, Department of Horticulture, JNKVV, Jabalpur. The AFRBD (Asymmetrical Factorial Randomized Block Design) was set up with 20 distinct treatment combinations of soil media Soil + Sand, Soil + Vermicompost, Soil + Sand + Vermicompost, Soil +Vermicompost + Biofertisol, Soil + Vermicompost + Azatobactor + PSB + KSB and plant growth regulator i.e. GA3 0 ppm (control), GA3 100 ppm, GA3 200 ppm, GA3 300 ppm. The vigour and germination of seeds were significantly impacted by the type of media used and the usage of plant growth regulators. In regard to the growth parameter, better values for seedling height, number of leaves and stem girth at 30, 60 and 90 days after sowing were found with treatment GA3 200 ppm. Growth metrics, such as root length, fresh shoot weight, dry shoot weight and dry root weight at 120 days, revealed improved results with a higher vigour index when the combination of GA3 200 ppm + growing medium (soil+ vermicompost+ Biofertisol) were applied. The GA3 200 ppm with growing medium (Soil+ Vermicompost+ Biofertisol) were proven to be better in terms of the seed germination parameters. Minimum time was taken for seed germination and increased seed germination percentage had been recorded at 30 days after planting.
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Objective. The germination capacity of Dioclea guianensis is being described. Materials and methods. The fruits were collected in the La Depresión district of La Sierra municipality, Cauca, where floristic and ecological restoration studies are being conducted. For the morphological description, 50 fruits and 75 seeds were used, and morphometric measurements of length, width, and thickness were taken. The stages of germination were observed and described through nursery sowing with four repetitions of 290 seeds each; observations were made daily and establishment was determined by the appearance of the second leaf. Results. Morphological description of the fruit and seeds: The fruit of D. guianensis is a dry, oblong-compressed legume with explosive dehiscence. The seeds are oblong-elliptical with a semicircular hilum, without a persistent funicle. Germination in D. guianensis is epigeal and phanerocotylar, with an average germination time of 25 to 40 days. The survival rate of seedlings until the appearance of the second leaf was 85%. Conclusion, D. guianensis exhibits a maximum germination rate of 99.8% and a seedling survival rate of 85%. The seedlings have phanerocotylar, epigeal, and straight germination, with fleshy cotyledons and the development of photosynthetic membranous leaf primordia. This allows the seedlings to develop in the first few weeks with the protection and storage of the cotyledons, and later assume the nutritional role of the photosynthetic leaf primordia.
Objetivo. Describir la capacidad de germinación de Dioclea guianensis. Materiales y métodos. Los frutos fueron colectados en el corregimiento La Depresión del municipio La Sierra Cauca, donde se adelantan estudios florísticos y de restauración ecológica. Para la descripción morfológica fueron utilizados 50 frutos y 75 semillas, se tomaron medidas morfométricas de largo, ancho y grosor. Las etapas de la germinación se observaron y describieron a partir de la siembra en vivero con cuatro repeticiones de 290 semillas cada una; las observaciones se realizaron cada día y el establecimiento se determinó por la aparición del segundo nomofilo. Resultados. Descripción morfológica del fruto y semillas: El fruto de D. guianensis, es seco de tipo legumbre oblongo-comprimidas con dehiscencia explosiva. Las semillas son oblongo-elípticas con hilo semicircundante, sin funículo persistente. La germinación es epigea fanerocotilar, el tiempo medio de germinación es de 25 a 40 días y el porcentaje de sobrevivencia de plántulas a la aparición del segundo nomofilo fue del 85%. Conclusiones. D. guianensis presenta una tasa máxima de germinación del 99.8%, una sobrevivencia del 85% de las plántulas, las cuales, al presentar una germinación fanerocotilar, epigea y recta, con cotiledones carnosos, y desarrollo de los primordios foliares membranosos fotosintéticos les permiten a las plántulas desarrollarse en las primeras semanas con la protección y almacenamiento de los cotiledones, para luego asumir el papel nutricional, los primordios foliares fotosintetizadores.
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Dioclea , Fabaceae , Germinación , Plantones , FrutasRESUMEN
Aims/ Objectives: Effect of soil propagation media and bio-fertilizers on seedling germination and seedling vigour in Aonla.Study Design: Completely Randomized Block Design.Place and Duration of Study: Research Field, Department of Fruit Science, College of Horticulture, Mandsaur, Madhya Pradesh during 20th February 2021 to 05th April 2021.Methodology: The experiment was laid out using Completely Randomized Block Design with 18 different combinations of growing media. In the preparation of media and filling of polybags the soil was sieved and mixed FYM, Vermicompost and Neemcake in the ratio of 1:1:1:1 then added the bio-fertilizers.Results: In this study we found that treatment GM18- Soil + Neemcake + Vermicompost + FYM + Rhizobium + Trichoderma spp. @ 2.5 g found minimum days taken to seed germination (10.00 days), maximum number of seedlings sprouted (13.32), maximum survival percent (81.03%), maximum germination percentage (94.54%), maximum seedling height (8.94 cm), maximum number of leaves per plant (7.27), maximum leaf area (17.80 cm2),maximum fresh weight of shoot (4.34 g), maximum dry weight of shoot (2.21 g), maximum length of roots (7.21 cm), maximum diameter of roots (1.05 mm), maximum fresh weight of roots (1.04 g), maximum dry weight of root (0.44 g), maximum speed of germination (7.56), maximum mean daily germination (3.53), maximum peak value (1.33), maximum germination value (4.68).Conclusion: On the basis of results obtained in present investigation it is concluded that treatment GM18 (Soil + Neemcake + Vermicompost + FYM + Rhizobium + Trichoderma spp. @ 2.5 g) was found to be the best for seed germination characters and seedling vigour characters.Directions of Further Use: The experiment should be done with different concentrations of growth regulator to find the best combination.
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A study on effect of pre-sowing seed treatments with organics, chemicals and plant growth regulators on seed germination and seedlings growth of papaya cv. Arka Surya was carried out at YSRHU- College of Horticulture, Anantharajupeta during the year 2021 - 2022, under complete randomized design with eighteen treatments and replicated thrice. The papaya seeds were subjected to various organic, chemicals and plant growth regulators treatments. Papaya seeds soaked in KH2PO4 @ 0.5% (T7) had recorded minimum days for initiation of germination (8.67) and 50% germination (10.34). The treated seeds had also recorded maximum germination percentage (86.66), germination index (1.16), dry weight of shoots (2.00 g), tap root length (26.33 cm), number of lateral roots per seedling (22.84), dry weight of roots (0.79 g), quality index of papaya seedlings (0.47) and seedling survivability (100.00%). In addition the same treatment exhibited higher chlorophyll content (3.51 and 3.91 mg/g), photosynthetic rate (15.32 and 17.76 µmol Co2m-2s-1), transpiration rate (4.61 and 5.69 mmol m-2 s-1) and stomatal conductance (2.73 and 4.12 mol m-2 s-1) at 35 and 45 DAS, respectively. While the lowest values for germination and growth parameters were observed in treatment salicylic acid @ 2 mM (T16). Among all the treatments, KH2PO4 @ 0.5% (T7) was found to be superior to the other treatments followed by KNO3 @ 0.5% for the enhancement of germination, growth and physiological traits.
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Wheat (Triticum aestivum L.) is the world's most essential staple food. It is the most important crop in Himachal Pradesh during the winter season in the state's low and mid hills. Manipulation of agronomic practices such as planting pattern/geometry and seed rate is regarded as the first stage in achieving optimum plant distribution over cultivated area, resulting in greater utilization of above and below ground natural resources. A field and laboratory investigation was undertaken to study the effect of different planting patterns and seed rates on its quality in wheat by evaluating the quality parameters. The experiment with twelve treatment combinations comprising of four planting patterns (P1-15 cm, P2 -23 cm, P3 -15 x 15 cm and P4 -23 x 23 cm) and three seed rates (S1 -100, S2 - 120 and S3 -140 kg/ha) was conducted in Randomized Block Design with four replications during Rabi season of 2015-16 at experimental farm of CSKHPKV, Palampur, Himachal Pradesh, India. Observations showed that 23 × 23 cm planting patterns produced significantly (2.96%) higher 1000 seed weight, (8.08%) seedling length, (6.01%) seedling dry weight, (8.34%) vigour index –I and (6.58%) vigour index- II over normal planting patterns (23 cm), whereas other planting techniques had no appreciable impact on germination percentage. Different seed rates had no appreciable impact on 1000 seed weight, germination rate, seedling length, seedling dry weight, and vigour index. Criss cross sowing (23 x 23 cm) with 140 kg per hectare is the best combination for quality seed production of wheat.
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Aim: The aim of the present study was to investigate the effect of different pre-sowing treatments on germination characteristics of strawberry guava (Psidium cattleainum Sabine).Study Design: The experiment consisted of eleven treatments and three replications laid out in a Completely randomized design (CRD).Place and Duration of Study: The study was conducted at Orchard Nursery, Department of Fruit Science, Horticulture College and Research Institute, Tamil Nadu Agricultural University, Coimbatore during April-June 2023.Methodology: Seeds of Psidium cattleainum (strawberry guava) were collected from guava germplasm from an orchard and treated with different pre-sowing chemicals. After pre-sowing treatment, the seeds were sown and various germination characteristics and growth parameters were observed and recorded.Results: The seeds treated with GA3 500 ppm for 30 minutes took minimum days to germinate (20), with higher germination percentage (79.33%), maximum vigour index I, and vigour index II (708.6 and 584.23). The growth parameters such as seedling height, stem girth,and number of leaves, primary root length, number of lateral roots were recorded higher in GA3 500 ppm followed by Thiourea 1%.The biomass production of seedlings was also higher in seeds treated with GA3 500 ppm.Conclusion: The seeds treated with GA3 500 ppm for 30 minutes recorded higher germination characteristics, growth parameters and biomass production. So for quicker propagation of strawberry guava to be used as rootstock, seeds can be treated with 500 ppm GA3 to obtain the large number of seedlings for the use of rootstock.
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Study was undergone to evaluate the efficacy of floatation grading on the seeds of Amaranthus Cv. PLR 1 using organic solvents in Department of Seed Science and Technology, TNAU, Coimbatore during March 2023.The experiment was laid out in Completely Randomized Design with four replications in each five treatments. We considered four organic solvents viz., Acetone, Methanol, Dichloromethane and Petroleum Ether along with water and observed the seed recovery percentage, germination percentage, root length, shoot length and vigour index of all the treatments. The results showed that sinker fraction regardless of organic solvent and water performed better than floater fraction. The grading ability of Dichloromethane was greater among the assessed organic solvents. The sinkers seed recovery percent of dichloromethane (93%) was higher than sinkers recovered by water (63%). Also, the sinkers of dichloromethane recorded higher germination percent(77%) and vigour index(304) than the sinkers of water whose germination percent is 55% and vigour index is 272.This specific gravity grading using dichloromethane can be adopted to segregate well filled and ill filled seeds in a seed lot of Amaranthus. This method of floatation grading is a good substitute for sieve grading which requires skilled labour and availability of specific size sieve to properly segregate seeds. So, this can be used as an alternate method for grading Amaranthus seeds.
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Aim: The present investigation was carried out to study the effect of gamma irradiation on the growth parameters of cowpea variety Paiyur-1.Seeds were irradiated with physical mutagen viz., gamma irridation. The doses of radiation used were control, 50 Gy, 100 Gy, 150 Gy, 200 Gy, 250 Gy, 300 Gy, 350 Gy, and 400 Gy.Finding the LD50 and GR50 value for the physical mutagen dosages is the study's major goal. Cs137 is exposed to gamma radiation, and the observation were recorded in the M1 generation.Study Design: The regression method by applying Probit analysis based on rates of fatalities was utilised to estimate the Lethal Dose (LD50). Simple Linear Regression on the dose-response curve was adopted to evaluate the GR50 incorporating all of the vegetative parameters using R software.Place and Duration of Research: The study was carried out at Horticulture orchard, Department of Vegetable Science, Horticulture College and research Institute, Tamil Nadu Agricultural University, Coimbatore during 2022-2023.Methodology: In this study, the paiyur-1 variety of cowpea was sown with two replications, those mutagenized seeds were planted individually in germination paper using the roll towel method and dry seeds that weren't irradiated served as the control. To find the lethal dose 50 and to observe the shoot length, root length, germination percentage, mortality rate and seed vigour.Results: Among the 9 gamma irradiated treatments, 50Gy and 100 Gy exhibited superior germination percentages, despite the fact 50 Gy treated seeds were having appealing shoot length, root length, and plant height. The linear relationship have given that shoot length and root length are the two parameter that are highly susceptible to gamma irradiation than plant height and seed vigour.
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In recent years, Hass avocados (Persea americana Mill.) have become more widely cultivated in Colombia. One of the difficulties facing the nation is boosting orchard output by ensuring graft plants have the best agronomic, genetic, and phytosanitary criteria. In this study, the growth and development of avocado rootstocks derived from seeds of various weight ranges will be monitored for 90 days. Four weights of avocado seeds (S1, S2, S3, S4) (30–40 g, 40–50 g, 50–60 g, and 60-70 g) and four scion maturity of (D1, D2, D3, D4) (60 days old, 70 days old, 80 days old, 90 days old were used as treatments in a factorial completely randomized experimental design. The outcome showed that the best germination rate of 75% for S4 and the highest survival percentage (94.98%) for S4D4. After 90th DAG the maximum number of new shoots per plant (5.75), number of leaves/plants (18.15), leaf area (20.83 cm2), and carbohydrate content (6.57%) were reported. Based on the results of the experiment, it was determined that the optimum method for propagating avocados was softwood grafting using 60–70 g seed size (rootstock) and 90-day-old scion. These plants had better vigor and faster germination rates, all of which could reduce the length of time spent in the nursery while still ensuring the quality of the material, which is influenced by improved seedling growth.
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Temperature is one of the factors which play a major role in deciding the planting value of a seed lot in the seed testing laboratory. The temperature requirement varies with species. High or low temperature may stimulate or inhibit the germination of seeds under testing. The tropical crops may require higher temperature than temperate crops for germination. Eventhough, a seed lot is having high germination and vigour parameter, it could be revealed with suitable seed testing temperature only. Since bhendi is a tropical vegetable crop, two varieties of bhendi, Arka Anamika and CO4 with each five different lots were tested with four different temperature regimes viz., 15, 20, 25 and 30ºC. The experimental results revealed that bhendi seeds performed well at the temperature regime of 30ºC followed by 25ºC which was on par with each other. It was also accompanied with seed quality characteristics of seedling length, vigour index and dry matter production of seedlings. Irrespective of varieties and lots studied, the high temperature showed its superiority over other temperatures. The study also disclosed that the low temperature of 15ºC was not suitable for bhendi seed germination, because none of the seeds produced normal seedling. At low temperature seeds could produce more abnormal seedlings than rest of the temperature regimes studied. It could be inferred that the seed testing temperature suitable for bhendi seed germination is 30°C or 25°C. Low temperature (15°C) is not suitable, since the normal seedlings were not produced at this temperature.
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Capparis decidua (Family: Capparidaceae) is a very nutritious shrub of arid region. Its fruits are utilized in famous Rajasthani Panchkutta and Tricutta vegetable and pickle making. The present paper deals with the effect of augmentation of Apis mellifera beehives on the fruit setting and fruit quality parameters. It was found that the average numbers of fruit setting raceme-1 were found 4.36, 3.77 and 2.2 in natural pollination with augmentation of honey bees, pollination by honey bees only and natural pollination respectively. Germination percentages, total sugar, average fruit weight, shoot length and seedling length was higher in case of natural pollination with augmentation of honey bees among all the treatments.
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The present experiment was conducted during Rabi 2019 at experimental field of Pulses Research unit, Dr. PDKV, Akola to assess the suitable priming treatment for chickpea crop (variety: JAKI-9218). The experiment was laid out in randomize block design in three replication. The morpho-physiological traits viz. germination %, plant height, number branches, dry matter content, number of day required to 50% flowering were taken.. The morpho-physiological i.e. plant height, number of branches, dry matter content, germination % showed significant increased in chickpea crop when seed priming was carried out with potassium nitrate for 4 hours. The numbers of days required to 50% flowering were recorded least in seed priming GA3 for 4 hours. Protein content also recorded significantly higher in seed priming with potassium nitrate for 4 hours. Yield and yield attributes were also significantly increased in seed priming with potassium nitrate for 4 hours. It is concluded that seed priming were with potassium nitrate for 4 hours recorded higher morpho-physiological characters over priming treatment and control.
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The present investigation was carried out to find influences of different sowing dates with application of GA3 on germination and seedling growth of Cape gooseberry (Physalis peruviana L.) in subtropical condition Prayagraj. The experiment was conducted in a Complete Randomized Design with three replications during August-March (2022-23) at Horticultural Research Farm, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences Prayagraj Uttar Pradesh India. The 10 treatments comprised of five dates of sowing i.e., T1: 20 August 2022 with seed treatment from GA3 @100ppm, T2: 20 August without seed treatment from GA3, T3: 5 September 2022 with seed treatment from GA3 @ 100 ppm, T₄: 5 September without seed treatment from GA3, T5: 20 September 2022 with seed treatment from GA3 @100ppm, T6: 20 September 2022 without seed treatment from GA3. T7: 5 October 2022 with seed treatment from GA3 @100ppm, T8: 5 October 2022 without seed treatment from GA3, T9: 20 October 2022 with seed treatment from GA3 @100ppm, T10: 20 October 2022 without seed treatment from GA3. From all the above treatments the highest percentage of germination was recorded from T7: 93.33% followed by T8: 90% and T10: 91.66% and vegetative growth of seedling was higher in T7 followed by T8 and T9. All the treatments are sown in nursery through seed propagation after that transplanted in main field level for observe germination parameters and vegetative growth of Cape gooseberry seedling influenced by different sowing dates.
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The evaluation of pollen viability and fruit setting capacity are two essential criteria for pollinator’s characterization. This study was carried out to evaluate pollen Fertility/ viability in five Varieties of Tomatoes. We analyzed the viability of pollen grains of five different varieties of tomatoes grown in ITM University, Gwalior, Madhya Pradesh, India. Aceto- carmine method is used to estimate pollen viability. The germination percentage, pollen viability and Fruit setting rate were recorded at different stages respectively. Pollen viability in relation to fruit set was analyzed while growth parameters and fruit yield per plant of each variety were also collected and analyzed. Results displayed that the highest pollen viability rate (100%) was obtained in SWEAKAR-448 Variety followed by Arka Vikas (95%), ARJUN-135 (93%), 9005-SIRI (90%) and PKM-1 (80%). The highest percentage of germination (95%) was shown by 9005-SIRI Variety and maximum fruit setting (15-20 Fruits per plant) was recorded in the SWEAKAR-448 Variety. The results showed significant vegetative growth variations and correlations between pollen viability and fruit setting characters. In addition, it was found a noticeable variation in Pollen viability/fertility, was not proportional to fruit setting percentage. In general Pollen viability was directly linked to fruit set, whereas formation and fruit set were not in the tomato varieties that were investigated. Pollen viability was high, which was accompanied through a reduction in the number of fruit sets, fruit size and pollen viability in the field is highly variable, indicating that differences in microenvironment may have a profound effect on fruit setting and pollen viability.
RESUMEN
Resumen Clostridioides difficile es un patógeno esporulado oportunista responsable de diarrea asociada a antibióticos en humanos. C. difficile produce 2 toxinas principales: TcdAy TcdB, además de la toxina binaria (CDT), también asociada a la virulencia. Este estudio buscó caracterizar el aislamiento ALCD3, involucrado en un episodio de recurrencia de una infección nosocomial. La caracterización molecular mostró que dicho aislamiento pertenece al toxinotipo 0/v y el análisis por MLST demostró un perfil alélico adk:91, atpA:1, dxr:2, glyA: 1, recA:27, sodA: 1 y tpi:1, lo cual corresponde al ST293 (MLST clado 1). Durante el crecimiento, el aislamiento ALCD3 mostró un incremento temprano de la tasa de esporulación y valores máximos de formas termorresistentes luego de 2 días de incubación. Tanto la cinética de esporulación como la producción de formas termorresistentes fueron más rápidas en el aislamiento ALCD3 que en la cepa de referencia VPI 10463. La germinación en presencia del germinante natural taurocolato fue más rápida en el aislamiento ALCD3 que en la cepa VPI 10463, lo que indica que aquel comienza la hidrólisis del córtex antes. También, el co-germinante glicina indujo una rápida liberación de ácido dipicolínico en ALCD3. Estos hallazgos indican que el aislamiento ALCD3 es particularmente eficiente en la esporulación y en la germinación. El presente trabajo representa el primer informe de la circulación de C. difficile ST293 en Argentina. La habilidad del aislamiento ALCD3 para producir toxinas y su alta capacidad de esporulación/germinación son características claves compatibles con un alto potencial de diseminación e inducción de infecciones recurrentes.
Abstract Clostridioides difficile is an opportunistic spore-forming pathogen responsible for antibiotic-associated diarrhea in humans. C. difficile produces two main toxins: TcdA and TcdB as well as a third toxin named binary toxin (CDT) that is also involved in virulence. The present study aimed at characterizing the C. difficile isolate ALCD3 involved in a relapse episode of nosocomial infection. Molecular characterization showed that isolate ALCD3 belongs to tox-inotype 0/v and the MLST analysis demonstrated allelic profile adk:91, atpA:1, dxr:2, glyA: 1, recA:27, sodA: 1 and tpi:1 which corresponds to ST293 (MLST clade: 1). During growth, isolate ALCD3 showed an early increase in the sporulation ratio as well as maximal values of heat resis-tant forms after 2 days of incubation. Both sporulation kinetics and production of heat resistant forms were faster for isolate ALCD3 than for the reference strain VPI 10463. Germination in the presence of the natural germinant taurocholate was faster for isolate ALCD3 than for strain VPI 10463, which indicates that isolate ALCD3 starts cortex hydrolysis earlier than strain VPI 10463. Furthermore, the co-germinant glycine, induces rapid release of dipicolinic acid (DPA) in isolate ALCD3. These findings indicate that isolate ALCD3 is particularly efficient in both sporulation and germination. The present work represents the first report of the circulation of C. difficile ST293 in Argentina. The ability of isolate ALCD3 to produce toxins and its high sporulation/germination capacity are key features compatible with a microorganism with high dissemination potential and the possibility of inducing recurrent infections.