COMPARISION OF DIAGNOSTIC ACCURACY OF NON – FASTING DIPSI AND HBAIC WITH FASTING WHO CRITERIA FOR DIAGNOSIS OF GESTATIONAL DIABETES MELLITUS

Background Gestational Diabetes Mellitus [GDM] is de?ned as Carbohydrate intolerance with recognition or onset during pregnancy and resolves postpartum. Prevalence of GDM in India varies from 3.8 - 21% with different demography and diagnostic methods used. As early diagnosis and control of maternal hyperglycaemia plays a vital role in prevention of adverse outcomes, universal screening is almost mandatory due to high prevalence, we need a simple economical, feasible test with higher sensitivity to diagnose GDM. To compare diagnostic accuracy of two non- Aim fasting tests DIPSI & HBAIC and fasting WHO criteria for diagnosis of GDM. To compare DIPSI with WHO criteria as Objectives standard. To compare HBA1C with WHO criteria as standard This study was done on 100 ANC cases to compare Results: diagnostic accuracy of DIPSI & HBAIC with fasting World Health Organization Glucose Tolerance Test. Mean age of participants was 27.18±4.60 years. 39% patients were in age group of 21 to 25 years and 34% patients were in age group of 26 to 30 years. Majority (45%) of the patients were in gestational age of 26 to 30 weeks. In this study, gestational diabetes mellitus was diagnosed in 47 (47%) patients according to WHO GTT, in 48 (48%) patients according to DIPSI and in 34 (34%) patients according to Glycated Haemoglobin. Mean gestational age of patients during diagnosis of gestational diabetes mellitus was 29.21±2.84 weeks by DIPSI, 28.83±2.82 weeks by WHO GTT and 29.29±3.15 weeks by Glycated Haemoglobin. Mean blood sugar parameters of gestational diabetes mellitus women were 174.96±16.58 mg/dl by DIPSI, 173.21±17.58 mg/dl by WHO GTT and 9.41±1.91 gm% by Glycated Haemoglobin. The sensitivity of DIPSI with regard to WHO GTT was 89.36%, speci?city 88.68%, positive predictive value 87.50%, negative predictive value 90.38%, diagnostic accuracy 89.00% and chi square value of 60.78. These values convey that DIPSI is as good as gold standard WHO GTT criteria. The sensitivity of Glycated Haemoglobin with regard to WHO GTT was 51.06%, speci?city 81.13%, positive predictive value 70.59%, negative predictive value 65.15%, diagnostic accuracy 67.00% and chi square value of 11.51. These values convey that Glycated Haemoglobin is not as good as gold standard WHO GTT. Based on ?ndings from this study it can be concluded that DIPSI is Conclusions: equally as good as World Health Organization Glucose Tolerance Test criteria in diagnosing gestational diabetes mellitus in antenatal women of south India. Since DIPSI does not require fasting it is more feasible than World Health Organization criteria. Glycated haemoglobin estimation is another test to detect diabetes mellitus which does not require fasting however its results are not close to gold standard WHO criteria unlike DIPSI

Gestational Transient Thyrotoxicosis in Pregnancy

Gestational Transient Thyrotoxicosis (GTT) presenting duringpregnancy is a common clinical tricky issues and can bechallenging to differentiate between physiologic patterns of thyroiddysfunction during gestation and fundamental hyperthyroidism.This review provides a instantaneous of the differential diagnosis,clinical presentation, diagnostic options, and impending contraryeffects of maternal thyrotoxicosis to the fetus and treatmentrecommendations for GTT ascending in pregnancy.

Comparative evaluation of screening tests for maternal and fetal outcome in gestational diabetes.

Background: We aimed to evaluate the predictive value of two screening tests - 50 gm GCT and 75 gm GTT for adverse maternal and fetal outcome in patients with gestational diabetes mellitus. Methods: This was a prospective study of 200 antenatal women booked at tertiary care hospital Delhi during the year 2010-2012. Pregnant females at 24-28 weeks gestation were randomly selected and subjected to 50 gm GCT and 75 gm GTT. The women’s history, clinical examination recorded, 50 gm GCT and 75 gm GTT values recorded and the two tests were compared for maternal and fetal outcome. Results: 75 gm GTT had higher specificity, positive and negative predictive values as compared to 50 gm GCT for both maternal and fetal outcome. However 50 gm GCT was more sensitive for predicting maternal and fetal complications. Conclusions: 75 gm GTT is better than 50 gm GCT for predicting adverse maternal and perinatal outcome in gestational diabetic patients.

Evaluación clínica del uso de nutrición enteral domiciliaria en pacientes pediátricos / Clinical evaluation in pediatric patients with home enteral nutrition

Introducción: La nutrición enteral (NE) es la entrega de nutrientes al tracto gastrointestinal mediante una sonda nasogástrica (SNG), nasoyeyunal (SNY) o de gastrostomía (GTT); siendo la ruta preferida para satisfacer las necesidades nutricionales cuando la vía oral está contraindicada o es insuficiente. Objetivo: Describir el perfil de pacientes pediátricos que requirieron NE domiciliaria y su efecto en el estado nutritivo. Sujetos y Método: Estudio analítico retrospectivo, en 37niños hospitalizados en el Servicio de Pediatría del Hospital Clínico Universidad Católica (UC) que fueron dados de alta con soporte nutricional enteral durante el período de Abril 2010 a Abril 2011. Resultados: La indicación principal para iniciar soporte nutricional fue trastorno de deglución (n=27), siendo la mayoría de los pacientes menores de 1 año al momento de recibir la indicación (n=27). El tipo de dispositivo más utilizado fue GTT (n=20). No hubo complicaciones asociadas al uso de SNG. Las complicaciones asociadas al uso de GTT fueron: granuloma periostomal (n=7), neumonía por aspiración (n=3) y retiro accidental (n=1). Del total de pacientes evaluados, un 62% presentaba desnutrición calórico-proteica al inicio de la NE versus 30% al último control (p<0,001). Conclusión: El uso de soporte nutricional enteral permite recuperar y/o mantener el estado nutricional de losniños con trastornos de la deglución y alteraciones en la curva del crecimiento, mejora la calidad de vida, y presenta una tasa baja de complicaciones mayores.

Enteral nutrition (EN) is the delivery ofnutrients to the gastrointestinal tract by nasogastric (SNG), nasojejunal (SNY) or gastrostomy (GTT), being the preferred route to fulfill nutritional needs when the oral route is contraindicated or inadequate. Objective: To describe the clinical profile of pediatric patients with home EN and to assess their nutritional status. Subjects and methods: A retrospective analytic study in 37 children from the Pediatric Service Hospital Clinico UC, discharged with enteral nutrition support from April 2010 to April 2011. Results: The main indication to use EN was swallowing disorder (n=27). 73% began EN before one year of life. GTT was the principal route to EN (n=20). There were no complications associated to NGT or OGT. The reported complications associated with GTT were periosteal granuloma (n=7), aspiration pneumonia (n=3) and accidental removal (n=1). 62% ofpatients had protein-calorie malnutrition (z-score W/H) at baseline vs. 30% at the last control (p<0,001). Conclusion: The use of enteral nutrition allows to restore and/ or to maintain nutritional status in children with swallowing disorder or undernutrition. This feeding procedure also improves the quality of life and promotes a proper development without major complications.

Gamma-tocotrienol Alters Protein Expression of HepG2 Cell Line

Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiprolifera-tive effects on a liver cancer cell line (HepG2) with an IC50 value of 170μM. In this study, two dimensional gel electrophoresis (2DE) was used to determine changes in protein expression in HepG2 cell line following treatment with GTT. The ultimate aim is to identify the possible molecular mechanisms involved in GTT antitumor activity. This study is focused on obtaining a 2DE protein profile for HepG2 cell line with and without GTT treatment. In the preliminary analysis of the resulting 2DE profiles, 18 protein spots were found to be differentially expressed in cells treated with GTT. This observation is confirmed by extending the analysis to a larger sample size. By studying the effects of GTT treatment on differential protein expression in HepG2 cells the underly-ing mechanisms involved in the antitumor activity of GTT may be elucidated.

Effect of Hot Spring Water Drinking on Glucose Metabolism / 日本温泉気候物理医学会雑誌

Drinking of hot spring water containing sulfur or sodium bicarbonate is reported to be beneficial for diabetic patients. In the present study, sulfur spring water and water from simple thermals were chosen to examine their effects on glucose metabolism. Eleven volunteers including eight type 2 diabetic patients and three healthy subjects drank hot spring water from Kawayu Onsen (K-O, acid alum vitriol hydrogen-sulfide springs, pH1.98), and another six patients from Asukano-yu (A-Y, alkaline simple thermals, pH8.9). Oral glucose tolerance test (OGTT) was performed after drinking tap water (200<i>ml</i>) or K-O spring water (5 times diluted) at a week interval (short term effect). Increments in plasma glucose levels were significantly lower (p<0.05) with predrinking of spring water than those with pre-drinking of tap water. To know the long term effect of drinking, patients drank K-O water twice daily for 4 weeks or A-Y water twice daily for a week. Hemoglobin A_1C levels decreased significantly (p<0.05) by drinking K-O water and the levels of 1.5-Anhydro-D-glucitol increased by A-Y water (NS). From these findings, it is suggested that drinking hot spring water from K-O and A-Y is beneficial for diabetic patients if they have no contraindications for drinking these kinds of spring water.