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Objective To study nuclear factor E-2 related factor 2(NrF-2) and heme oxygenase -1(Ho-1) expression and to explore its clinical significance.Methods From February 2016 to June 2016, 176 cases human gastric tissue paraffin blocks selected in department of pathology in Hangzhou Cancer Hospital, including 78 cases of gastric cancer, 78 cases of normal tissue were analyzed.And the expressions of NrF-2 and Ho-1 were detected by immunohistochemistry in two groups of human gastric tissue.Results The levels of NrF-2 and Ho-1 in gastric cancer group were(0.752 ± 0.098),(0.862 ±0.081) were significantly higher than those in normal group(0.381 ±0.068),(0.412 ±0.083);NrF-2, the positive expression rate of Ho-1 in gastric wall invasion degree of T3 +T4 with lymph node metastasis, NTM stage III and IV gastric cancer tissues were significantly increased.The differences were statistically significant ( P <0.05 ) .Conclusion NrF-2 and Ho-1 play the important role in the development of gastric cancer and NrF-2, the positive expression rate of Ho-1 in the gastric carcinoma and gastric wall invasion, lymph node metastasis and NTM staging are closely related.The clinical diagnosis if the combined detection of NrF-2 and Ho-1, is conducive to the early diagnosis of gastric cancer and the prognosis of the disease.
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BACKGROUND: A soluble fragment of the c-erbB-2 oncoprotein become detectable in the serum of the gastric cancer patients by enzyme-linked immunosorbent assay(ELISA). METHODS: Serum c-erbB-2 levels of 67 gastric cancer patients are measured. In 27 patients, tissue levels of c-erbB-2 in paired normal and gastric cancer tissues are measured by ELISA(Triton Diagnostics Inc., USA). RESULTS: As 14 U/mL for positivity, the sero-positivity of the c-erbB-2 was 33%(22/67). The positivity showed no differences in cancer stages and tumor differentiations(well-moderate 29%, poor-signet ring cell type 35%). Tissue c-erbB-2 level was 390+/-284 U/mL and 568+/-536 U/mL in normal and cancer tissue, respectively. Blood c-erbB-2 level did not correlate with either cancer tissue c-erbB-2 level or normal tissue c-erbB-2 level. Nineteen out of 27 patients showed higher c-erbB-2 levels in cancer than normal tissues. The positivity of c-erbB-2 in serum was 7 out of 19 c-erbB-2 positive cancer tissue, whereas the positivity in serum was one out of 8 c-erbB-2 negative cancer tissue. CONCLUSIONS: Elevated blood c-erbB-2 oncoprotein level might reflect elevated c-erbB-2 level of gastric cancer tissue.