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Objective: To assess the genetic diversity of Gentiana straminea (Gentianaceae). Methods: Intersimple sequence repeat (ISSR) markers were used. Twenty-eight populations (83 individuals) of G. straminea were sampled from Sichuan, Qinghai, Gansu provinces, and Autonomous Region of Tibet. ISSR data were analyzed with the program POPGEN, and a UPGMA dendrogram was constructed. Results: PCR products corresponding to 183 alleles at 95 loci, amplified by the seven primers were scored. Eighty-eight loci were polymorphic. Expected heterozygosity (He) and Shannon's information index (Im) were 0.288 2 and 0.437 1, respectively. Nei's coefficient of genetic differentiation (Gst) and gene flow (Nm) were 0.678 3 and 0.237 1 at the population level, respectively. The genetic distance varied from 0.074 3 to 0.490 0. G. straminea populations were mainly divided into two braches by UPGMA tree. Conclusion: Genetic diversity level of G. straminea is high, and genetic diversity level among populations of G. straminea is higher than that in one population; Its autogenous variation mainly exist among populations; Hereditary capacity has certain correlation with geographical distribution. This work contributes basic information for variety identification, species in situ conservation, and enviroment investigation on the influence of heredity differentiated and Chinese Materia Medica genuine study. The congruence between genetic distance and geographic distances is high.
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Objective: To study the genetic diversity of 17 samples of Gentiana straminea from different habitats of Tibet. Methods: Sampling in populations was carried out, and the related species G. crassicaulis from the same area was as the out group; nr DNA ITS sequences were amplified and analyzed; UPGMA systematic tree was constructed. Results: One polymorphic site of G. straminea from Tibet was found; the six samples of out group have the identical DNA ITS sequences. Conclusion: The difference between G. straminea and the out group is obvious; seventeen samples of G. straminea from different habitats are divided into two groups; genetic diversity is more abundant; the result could provide the basic reference for the quality evaluation and the construction of core germplasm of G. straminea in Tibet.
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Objective To study the non-iridoid constituents from the roots of Gentiana straminea.Methods The compounds were repeatedly separated and purified on column chromatography of silica gel,Sephadex LH-20,and ODS,and their structures were identified on the basis of spectral and chemical methods.Results Twelve compounds were isolated from 70% ethanol extract of G.straminea and were identified as(-)-syringaresinol-4,4′-bis-?-O-D-glucopyranoside(1),gentiaphyllide-D(2),erythrecentaurin(3),macrophyiioside D(4),gentiaphyllide-E(5),roburic acid(6),uvaol(7),?-sitosterol(8),daucosterol(9),gentianose(10),?-glucose(11),and ?-glucose(12),respectively.Conclusion Compounds 1-6 and 10 are isolated from this plant for the first time.
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Objective To establish the fingerprint of Gentiana straminea from Qinghai Province by HPLC. Methods By orthogonal design and related methods based on information theories and chemometrics to optimize the proper HPLC conditions so that the fingerprint spectra of ten groups of different samples have been formed. Results The experimental results are the fact that the common fingerprint peaks, precision, and repeatability in the fingerprint totally meet the relative regulations required in Technical Requirements of the Fingerprint Research in Injection of Chinese Materia Medica (Tentatine Standard). Conclusion The chromatographic fingerprint can be used to identify and evaluate the quality of G. straminea.
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AIM: To establish the HPLC fingerprint of Genti ana straminea Maxim. METHODS: It was adopted gradient with Methanol and 0.4% phospho ric acid, the detection wavelength was at 230nm and recording time. And it w as compared with a software published by Zhejiang University. RESULTS: The constituents of Gentiana straminea Maxim. were well se parated by HPLC, and the similarity was more than 0.90 . CONCLUSION: The method can be used as the fingerprint of G entiana straminea Maxim.