Phylogeography of Paris poliphylla var. yunnanensis based on chloroplast gene trnL-trnF sequences / 中国中药杂志

Phylogeography is a research hotspot in the field of the genetic diversity and core germplasm construction of endangered rare plants. Paris polyphylla var. yunnanensis is a rare plant species mainly distributed in China. Wild individuals have been overexploited for the last few decades because of increasing demand for such medicines. Therefore, it is great significance to study the phylogeography of P. poliphylla var. yunnanensis based on chloroplast gene trnL-trnF sequences. In this study, chloroplast genes trnL-trnF were used in the phylogeography analysis of 15 wild and 17 cultivated populations of P. polyphylla var. yunnanensis. This study revealed that based on the results of neutrality tests and mismatch analysis, the rapid expansion of wild population has not been detected in P. polyphylla var. yunnanensis. After aligning and sorting the obtained cpDNA sequences, a total of 15 haplotypes were detected in all 32 populations. One haplotype was unique to the wild population, and 5 haplotypes were unique to the cultivated population. It can be seen that the haplotype richness of cultivated population was higher than that of wild population. The wild populations of P. polyphylla var. yunnanensis were divided into two groups according to evolutionary relationship of haplotypes and distribution map of haplotypes. The haplotype of branch Ⅰ was mainly distributed in Guizhou, and the haplotype of branch Ⅱ was located in Yunnan and Huidong, Sichuan. Therefore, it's speculated that Guizhou and the west Yunnan region may be glacial refuge in the evolutionary history of wild populations of P. polyphylla var. yunnanensis, and in order to protect the wild resources more effectively, wild populations of P. polyphylla var. yunnanensis in these two areas should be included in the protection zone.

African elephant genetics: enigmas and anomalies

During the last two decades, our understanding of the genetics of African elephant populations has greatly increased. Strong evidence, both morphological and genetic, supports recognition of two African elephant species: the savanna elephant (Loxodonta africana) and the forest elephant (L. cyclotis). Among elephantids, phylogeographic patterns for mitochondrial DNA are highly incongruent with those detected using nuclear DNA markers, and this incongruence is almost certainly due to strongly male-biased geneflow in elephants. As our understanding of elephant population genetics has grown, a number of observations may be considered enigmatic or anomalous. Here, several of theseare discussed. (i) There are a number of within-species morphological differences purported to exist among elephants in different geographic regions, which would be difficult to reconcile with the low genetic differentiation among populations. (ii) Forest elephants have a higher effective population size than savanna elephants, with nuclear genetic markersmuch more diverse in the forest elephants than savanna elephants,yet this finding would need to be reconciled with thelife history of the two species. (iii) The savanna and forest elephants hybridize and produce fertile offspring, yet full genome analysis of individuals distant from the hybrid zone suggests that gene flow has been effectively sterilized for atleast ∼500,000 years. (iv) There are unexplored potentialramifications of the unusual mito–nuclear patterns among elephants. These questions are considered in light of highmale and low female dispersal in elephants, higher variance of reproductive success among males than females, and of habitat changes driven by glacial cycles and human activity

Bioprospecting of plant growth promoting psychrotrophic Bacilli from the cold desert of north western Indian Himalayas.

The plant growth promoting psychrotrophic Bacilli were investigated from different sites in north western Indian Himalayas. A total of 247 morphotypes were obtained from different soil and water samples and were grouped into 43 clusters based on 16S rDNA-RFLP analysis with three restriction endonucleases. Sequencing of representative isolates has revealed that these 43 Bacilli belonged to different species of 11 genera viz., Desemzia, Exiguobacterium, Jeotgalicoccus, Lysinibacillus, Paenibacillus, Planococcus, Pontibacillus, Sinobaca, Sporosarcina, Staphylococcus and Virgibacillus. With an aim to develop microbial inoculants that can perform efficiently at low temperatures, all representative isolates were screened for different plant growth promoting traits at low temperatures (5-15°C). Among the strains, variations were observed for production (%) of indole-3-acetic acid (20), ammonia (19), siderophores (11), gibberellic acid (4) and hydrogen cyanide (2); solubilisation (%) of zinc (14), phosphate (13) and potassium (7); 1-aminocyclopropane-1-carboxylate deaminase activity (6%) and biocontrol activity (4%) against Rhizoctonia solani and Macrophomina phaseolina. Among all the strains, Bacillus licheniformis, Bacillus muralis, Desemzia incerta, Paenibacillus tylopili and Sporosarcina globispora were found to be potent candidates to be developed as inoculants as they exhibited multiple PGP traits at low temperature.

Phytochemical screening and evaluation of analgesic and antiinflammatory activities of Phaseolus vulgaris linn., seeds in rodents.

With an objective of developing some novel analgesic and antiinflammatory natural agents with fast acting and low toxicity profile here in, the different extracts of Phaseolus vulgaris (Linn) seeds were evaluated for analgesic and antiinflammatory activities using glacial acetic acid induced writhing and carrageenan induced rat paw oedema method respectively. For screening of the extracts for analgesic and antiinflammatory activities aspirin and diclofenac were used as standard drugs respectively. Petroleum ether extract exhibited significant analgesic and antiinflammatory activities. The petroleum ether extract can be considered as a potential candidate for analgesic and antiinflammatory activities. The presence of steroids and flavonoids in petroleum ether extract of Phaseolus vulgaris Linn., seeds could be attributed for the analgesic and antiinflammatory activities.

Trans-Amazon dispersal potential for Crotalus durissus during Pleistocene climate events / Potencial de dispersión trans-Amazónica de Crotalus durissus durante el Pleistoceno

Two disjunct distributional areas of Crotalus durissus (Neotropical rattlesnake) are in open habitats north and south of the Amazon Basin and are presently separated by humid rainforest habitats. We used ecological niche modeling to identify and investigate potential dispersal pathways for this species between the two areas during the late Pleistocene. Niches estimated for the two populations did not differ significantly. Our analyses indicated two possible, but a single most likely, potential routes of dispersal during the last glacial cycle. These results are important to understanding the history of Amazon Basin humid forest biotas, as they suggest agents of isolation among putative humid forest refugia in the form of dry forest and scrub, and associated biotas.

Actualmente existen dos áreas de distribución disyuntas de la serpiente de cascabel Crotalus durissus, afín a hábitats abiertos, al norte y al sur de la cuenca del Río Amazonas, separadas por selvas húmedas. Usamos técnicas de modelado de nicho ecológico para identificar corredores potenciales de dispersión para esta especie entre las dos áreas en el Pleistoceno tardío. Los nichos estimados para las poblaciones de cada una de las áreas de distribución no presentaron diferencias significativas. Nuestros análisis identificaron un corredor de dispersión más probable para esta especie durante el Último Máximo Glaciar. Estos resultados tienen implicaciones para el entendimiento de la historia de las biotas de las selvas húmedas del Amazonas, ya que sugieren causas de aislamiento entre refugios potenciales de selva húmeda, en la forma de selva seca y matorral.

Phylogeography of Polygala tenuifolia inferred from chloroplast DNA trnL intron sequence variation / 中草药

Objective: To study the molecular phylogeography of natural populations of Polygala tenuifolia (Polygalaceae) in China, reveal the forming reasons of geographic distribution pattern of extent populations, and further determine the potential glacial refuges and colonization/migration routines of P. tenuifolia during the postglacial or interglacial periods. Methods: We investigated the distribution patterns of genetic variation of 308 individuals from 39 natural populations of P. tenuifolia in China based on one chloroplast DNA fragment (cpDNA trnL intron). Results: A total of 26 polymorphic sites were identified and 12 cpDNA haplotypes were recovered. Phylogenetic analysis of cpDNA haplotypes suggested that the natural populations of P. tenuifolia could be divided into two different groups: northern part (including populations of northeast, center, and northwest of China) and southern part. There were no shared cpDNA haplotypes between the two groups. And the population genetic analysis suggested that the high level of genetic differentiation (Gst = 0.783, P < 0.001) between the two groups, the high level of the genetic diversity were found (Ht = 0.755) at the species level. No significant phylogeographic structure was detected in the North group. Conclusion: The present studies revealed that the multiple glacial refuges of P. tenuifolia are identified in the northern and southern parts of China during the Quaternary periods. And northern populations have occurred the expansion events during the post-glacial or interglacial periods. The genetic divergence between the north and south groups of natural populations of P. tenuifolia may be caused by the long term geographic isolation.

Current and historical climate signatures to deconstructed tree species richness pattern in South America / Efeitos dos climas atual e histórico no padrão desconstruído de riqueza de espécies arbóreas na região Neotropical

The purpose of this study was to investigate the importance of present and historical climate as determinants of current species richness pattern of forestry trees in South America. The study predicted the distribution of 217 tree species using Maxent models, and calculated the potential species richness pattern, which was further deconstructed based on range sizes and modeled against current and historical climates predictors using Geographically Weighted Regressions (GWR) analyses. The current climate explains more of the wide-ranging species richness patterns than that of the narrow-ranging species, while the historical climate explained an equally small amount of variance for both narrow-and-wide ranging tree species richness patterns. The richness deconstruction based on range size revealed that the influences of current and historical climate hypotheses underlying patterns in South American tree species richness differ from those found in the Northern Hemisphere. Notably, the historical climate appears to be an important determinant of richness only in regions with marked climate changes and proved Pleistocenic refuges, while the current climate predicts the species richness across those Neotropical regions, with non-evident refuges in the Last Glacial Maximum. Thus, this study's analyses show that these climate hypotheses are complementary to explain the South American tree species richness.

O objetivo deste estudo foi testar qual dos climas, atual ou histórico, é o principal preditor do padrão atual de riqueza de espécies arbóreas de interesse comercial. Nós modelamos a distribuição de 217 espécies usando Maxent e usamos esses mapas preditivos para obter o padrão de riqueza de espécies. A riqueza foi desconstruída em relação ao tamanho da distribuição geográfica das espécies e modelada contra os climas atual e histórico utilizando Regressões Geograficamente Ponderadas. O clima atual explicou melhor o padrão de riqueza das espécies com ampla distribuição geográfica do que de espécies com distribuição restrita, enquanto o clima histórico explicou a mesma variância na riqueza dos dois grupos de espécies. Nossas análises com plantas sul americanas mostram diferentes relações da riqueza de espécies ampla e restritamente distribuídas com os climas atual e histórico, quando comparado aos resultados encontrados no hemisfério norte. O clima histórico se mostra como importante preditor da riqueza somente em regiões com mudanças climáticas acentuadas e onde ocorreram refúgios Pleistocênicos, enquanto o clima atual é o melhor da riqueza nas regiões Neotropicais sem evidências de refúgios durante o máximo da ultima glaciação. Dessa maneira, nossos resultados indicam que essas hipóteses são complementares para explicar a riqueza predita de espécies arbóreas da América do Sul.

Improvement of the current routine method of WBC counting in cerebrospinal fluid / 重庆医学

Objective To improve the reliability and accuracy of WBC counting in cerebrospinal fluid (CSF) ,this article is stud-ying the improved method of WBC counting in CSF by finding out the optimum percentage of CSF specimen with the most suitable concentration of acetic acid .Methods CSF specimen was mixed with different acetic acid at different ratio respectively .WBC counts were performed in 5 minutes on diluted samples of various concentrations .A series of 20 CSF specimens were analyzed via the proposed assay and conventional method .The average value and coefficient of variation (CV) of WBC count of each sample were c compared and analyzed .Results The optimum percentage of CSF sample was obtained at 60∶40 ratio .In this percentage , the maximal WBC count (189/μL) was obtained compared that of conventional method (161/μL) .Moreover ,the CV of the WBC counts in this percentage (7% ) was also lower than that of the conventional method (18% ) .Conclusion The reliability and accur-ancy of WBC counting in CSF was the optimum percentage of CSF specimen and 5% acetic acid was 60 :40 .It may lead to a more reliable ,accurate and standard way of WBC counts in CSF .

The Clinical Characteristics and Prognosis after Acute Ingestion of Glacial Acetic Acid

PURPOSE: A retrospective study with a literature review was conducted to identify the clinical characteristics and prognosis after the acute ingestion of glacial acetic acid. METHODS: The medical records of 20 patients,who had presented to the emergency department of Ajou University Hospital complaining of the acute ingestion of glacial acetic acid between January 2006 and December 2011, were examined retrospectively. RESULTS: Among the 172 patients admitted for caustics injury, 20 patients ingested glacial acetic acid. The mean age of the patients was 55+/-23.5, and the mean volume of the acid was 84.5+/-71.3 ml. The clinical features included 1) oral ulcers in 12 patients (63.2%), 2) respiratory difficulties in 11 patients (57.9%), 3) oliguria in 8 patients (42.1%), 4) renal toxicity in 7 patients (36.8%), 5) hepatic failure in 7 patients (36.8%), 6) disseminated intravascular boagulopathyin 7 patients (36.8%), 7) low blood pressure in 8 patients (42.1%), and 8) mental changes in 9 patients (47.4%). Ten patients required endotracheal intubation. Nine patients were admitted to the intensive care unit, and 5 patients expired. CONCLUSION: The ingestion of glacial acetic acid can cause severe symptoms, such as metabolic acidosis, multiple organ failure and upper airway swelling frequently and has a high mortality rate. Therefore, aggressive treatment, including endotracheal intubation, should be considered at the early stages.

A Case of Successful Treatment for Acute Complication Caused by Ingestion of a Large Amount of Glacial Acetic Acid

Glacial acetic acid, a caustic substance of strong acidity, can easily be found in our daily lives; sometimes, it is drunk for the purpose of suicide, or it can be misjudged as a beverage. Glacial acetic acid is corrosive and toxic, therefore, even a tiny amount may cause fatal damage, such as gastrointestinal injury and systemic complication. A 66-year-old male patient, based on the wrong information, that glacial acetic acid is a healthy beverage, ingested 250 mL of 99% glacial acetic acid. Complications included gastric perforation, hemolysis, disseminated intravascular coagulation, acute renal failure, and liver dysfunction. We achieved successful treatment through performance of emergency surgery and hemodialysis on the patient, who had ingested a more than fatal dose of glacial acetic acid; herein, we would like to report on the case along with a review of the literature.

Geología glacial y postglacial del valle de la quebrada Saisay Andes centrales venezolanos (estado Mérida) / Glacial and postglacial geology of Saisay creek valley central venezuelan Andes (Mérida state)

Para el valle de la quebrada Saisay en Los Andes centrales venezolanos, fueron analizados los procesos y factores que intervinieron en su evolución glacial y postglacial. La metodología utilizada consistió en la realización de trabajos de campo, fotointerpretación, representación cartográfica, descripción y análisis de las facies sedimentarias para interpretar los procesos geológicos y geomorfológicos que afectaron el área. Los resultados indican principalmente la presencia de formas de erosión glacial (circos, aristas, valles en forma de U, escalones rocosos, estrías glaciales) y depósitos morrénicos neoglaciales (facies IA- 1-b). Igualmente, en el área se localizan sedimentos postglaciales de origen fluvioglacial (facies II-B-1-b) glaciodeltáicos (facies II-B-2-a y facies II-B-2-b) y depósitos glaciolacustrinos (facies II-B-2-c). El modelo de sedimentación general propuesto para esta secuencia sedimentaria se relaciona con un sistema de depositación asociado a un delta bifurcado (braid delta). Una edad mínima de 8.000 ± 500 años (TL) A.P., indica que este proceso de sedimentación pudo haberse iniciado a principios del Holoceno. En conclusión, la región estudiada se caracteriza por la presencia de formas típicas de erosión glacial, depósitos de origen glacial alpino y sedimentos postglaciales.

For the valley of the Saisay creek in the central Venezuelan Andes, the processes and factors that intervened in it´sglacial and postglacial evolution were analyzed. The methodology used consisted in photo-interpretation and detailed field works to draw the geomorphological map, and description and analysis of the sedimentary facies to explain the geologic and geomorphologic processes that affected the area. The results indicate mostly the presence of glacial erosion forms (circus, edges, U shaped valleys, rocky steps, glacial grooves) and neoglacial morrenic deposits (facies I-A-1-b). As well, there are postglacial sediments of fluvial-glacial origin (facies II-B-1-b), glacial-deltaic (facies II-B-2-a and facies II-B-2-b) and glacial-lacustrine (facies II-B-2-c). The model of general sedimentation proposed for this sedimentary sequence is related to a system of deposit associated to a bifurcated delta or braid delta. A minimal age of 8,000 ± 500 years (TL) A.P., indicate that this sedimentation process could have started at the beginning of the Holocene. In conclusion, the studied region is characterized by the presence of typical shapes of glacial erosion. Deposits of glacial alpine origin and postglacial sediments.

A Case of Acute Renal Failure Caused by Ingestion of Glacial Acetic Acid / 대한신장학회잡지

Acetic acid is a colorless liquid with a pungent vinegar-like order. Glacial acetic acid is 99% acetic acid. Acetic acid may lead to different effect on the damaged organ. Acetic acid ingestion is most common and results in pharyngeal, esophageal and GI burns, bleeding and volume depletion. Systemic effect include hemolysis, hepatic dysfunction, hypotension, renal failure and disseminated intravascular coagulation after ingestion of 90-100% acetic acid. Acute renal failure in acetic acid poisoning is rare and the mechanism of acute renal failure remains unclear. But tubular toxic effect of myoglobin or hemoglobin and direct action of acetic acid are suggested as the mechanism. Because of the acute renal failure may be fatal, the immediate treatment of hemolysis, substitution of blood and clotting factor and hemodialysis lead to improvement in general condition and renal function.