Effect of hypothermic machine perfusion on the metabolism of liver in rats / 中华肝胆外科杂志

Objective To examine the metabolic variations in liver after 30 min warm ischemic injury,and the effect of hypothermic machine perfusion on the metabolism in rats.Methods 40 SD male rats were randomly divided into 4 groups:Group A,the liver underwent warm ischemia for 0 min and cold storage (CS) for5 h; Group B,the liver was treated by warm ischemia for0 min,CS for4 h and then machine perfusion (MP) for 1 h; Group C,the liver suffered from warm ischemia for 30 min and CS for 5 h; and Group D,the liver was treated by warm ischemia for 30 min plus CS for 4 h plus MP for 1 h.During the MP process,the perfusion resistance index was recorded every 10 min,and the liver glycogen content and malondialdehyde (MDA) value were also detected.Results The hepatic glycogen content decreased after MP treatment,but there was no statistical significance (P ＞ 0.05).No differences on MDA contents was found between Group A and B (P ＞ 0.05),while MDA in Group D was significantly higher than that in Group C (P ＜ 0.05).After hypothermic MP treatment,the liver resistance index value was significantly reduced.Conclusion MP could reduce the resistance index but increase metabolic rate in liver undergoing warm ischemic injury,thus producing more lipid peroxides.

Antioxidant properties of Coscinium fenestratum stem extracts on Streptozotocin induced type 1 diabetic rats.

The aim of this investigation was to study the antioxidant status of Streptozotocin induced diabetic rats which were treated with methanolic stem extract of Coscinium fenestratum. Diabetes was induced by administering STZ by intra femoral vein injection at a dose of 40mg/kg body weight. Methanolic extract of Coscinium fenestratum was administered to the rats at a dose of 200 mg/kg body weight for a period of two weeks. The antioxidant status of the treated animals were assessed by measuring the activity of liver enzymes such as Catalase, Super oxide dismutase and Glutathione S Transferase. Levels of these antioxidant enzymes decreased in untreated diabetes compared to normal values. Administration of methanolic fractions of Coscinium fenestratum in STZ induced diabetic rats reversed these changes. Oxidative stress plays a major role in generation of free radicals in the pathogenesis of diabetes and its complications. Our findings suggest that C. fenestratum methanolic extract exerted anti-hyperglycemic activity by combating the oxidative stress, improving the glycogen content and by activating the carbohydrate metabolizing enzyme Hexokinase. The presence of Berberine and other phenolic compounds in the stem extracts may be responsible for this action.

Carbohydrate metabolism alterations in Biomphalaria glabrata infected with Schistosoma mansoni and exposed to Euphorbia splendens var. hislopii latex

This paper evaluates the alterations in the glycogen content of tissues (digestive gland and cephalopedal mass) and glucose in the haemolymph of Biomphalaria glabrata BH strain infected with Schistosoma mansoni BH strain and exposed to the latex of Euphorbia splendens var. hislopii. A reduction in the glycogen deposits was observed in infected snails exposed and not exposed to latex. However, the exposure to latex caused a greater depletion of the glycogen levels in both sites analysed, especially from the third week onward. The utilisation of latex as a molluscicide to control the population of infected B. glabrata selectively is proposed.

The effect of a low dose of clenbuterol on rat soleus muscle submitted to joint immobilization

The aim of the present study was to evaluate the effect of joint immobilization on morphometric parameters and glycogen content of soleus muscle treated with clenbuterol. Male Wistar (3-4 months old) rats were divided into 4 groups (N = 6 for each group): control, clenbuterol, immobilized, and immobilized treated with clenbuterol. Immobilization was performed with acrylic resin orthoses and 10 µg/kg body weight clenbuterol was administered subcutaneously for 7 days. The following parameters were measured the next day on soleus muscle: weight, glycogen content, cross-sectional area, and connective tissue content. The clenbuterol group showed an increase in glycogen (81.6 percent, 0.38 ± 0.09 vs 0.69 ± 0.06 mg/100 g; P < 0.05) without alteration in weight, cross-sectional area or connective tissue compared with the control group. The immobilized group showed a reduction in muscle weight (34.2 percent, 123.5 ± 5.3 vs 81.3 ± 4.6 mg; P < 0.05), glycogen content (31.6 percent, 0.38 ± 0.09 vs 0.26 ± 0.05 mg/100 mg; P < 0.05) and cross-sectional area (44.1 percent, 2574.9 ± 560.2 vs 1438.1 ± 352.2 µm²; P < 0.05) and an increase in connective tissue (216.5 percent, 8.82 ± 3.55 vs 27.92 ± 5.36 percent; P < 0.05). However, the immobilized + clenbuterol group showed an increase in weight (15.9 percent; 81.3 ± 4.6 vs 94.2 ± 4.3 mg; P < 0.05), glycogen content (92.3 percent, 0.26 ± 0.05 vs 0.50 ± 0.17 mg/100 mg; P < 0.05), and cross-sectional area (19.9 percent, 1438.1 ± 352.2 vs 1724.8 ± 365.5 µm²; P < 0.05) and a reduction in connective tissue (52.2 percent, 27.92 ± 5.36 vs 13.34 ± 6.86 percent; P < 0.05). Statistical analysis was performed using Kolmogorov-Smirnov and homoscedasticity tests. For the muscle weight and muscle glycogen content, two-way ANOVA and the Tukey test were used. For the cross-sectional area and connective tissue content, Kruskal-Wallis and Tukey tests were used. This study emphasizes the importance of anabolic pharmacological...

Glycogen Contents in Skeletal Muscles in Men and Different Species of Experimental Animals / 대한정형외과학회잡지

The normal values of glycogen contents in skeletal muscles in commonly used experimental animals and men were studied. In addtion, this study was designed to know the relationship between amount of exercise and glycogen content in muscle, and to know the causes of species difference. Glycogen contents were determined in quadriceps, gastrocnemius, and triceps in mouse, rat, rabbit, and man. The results of this study as follows: The value of glycogen content in human skeletal muscle is significantly higher than that of experimental animals. The heavier the body weight of experimental animal, the higher the value of glycogen content in each muscle. The value of glycogen content in gastrocnemius is higher than that of quadriceps. The value of glycogen content in quadriceps is higher than that of triceps.