Mycobacterium chelonae infection complicating traumatic and surgical wounds: A case series

Mycobacterium chelonae is a rapidly growing non-tuberculous mycobacterium. The skin and soft tissue infections due to this organism are steadily on the rise and need to be delineated specifically as most of these are not responsive to routine antituberculosis treatment. Here, we report 3 different presentations caused by Mycobacterium chelonae in traumatic and surgical wounds. Mycobacterium chelonae can complicate surgical or traumatic wounds.This infection may also present as injection site abscesses. Diabetics on insulin injections are especially at risk. A high index of suspicion is necessary in long standing culture negative lesions for clinching the diagnosis. PCR can be helpful in confirming the diagnosis.

Propagación in vitro de materiales seleccionados de Rubus glaucus Benth (mora de Castilla) en la provincia de Pamplona, región nororiental de Colombia / Propagation in vitro of selected materials of Rubus glaucus Benth (mora de Castilla) in the province of Pamplona, northeastern Colombia

En este estudio los materiales fueron seleccionados en 53 fincas pertenecientes a cuatro asociaciones de cultivadores de mora en los municipios de Pamplona y Chitagá (Norte de Santander, Colombia). Se emplearon como explantes segmentos nodales, los cuales permitieron obtener en poco tiempo brotes adventicios adecuados para la multiplicación masiva. Para la etapa de establecimiento se empleó el medio de cultivo Murashige y Skoog, (MS 1962), suplementado con ácido giberélico (GA3) (0.0-0.1 mg/L) y 6-benzil aminopurina (BAP) (0,0-2,0 mg/L), en la etapa de multiplicación MS ,suplementado con GA3 (0,0-0,03 mg/L-1) y BAP (0,0-2,5 mg/L) y para la etapa de enraizamiento MS, suplementados con ácido indolbutírico (AIB) (0,0-1,0 mg/L). A los datos generados en las tres etapas, se les aplicó un diseño experimental de bloques completos al azar y se analizaron estadísticamente los promedios de los tratamientos mediante una prueba de Tukey. Los resultados alcanzados indicaron tasas promedio de contaminación (16,5-49,7 %) multiplicación (3,8-4,3 brotes/explante) y enraizamiento (3,3-4,3 raíces/planta) in vitro para los diferentes materiales seleccionados y evaluados. Estos resultados, logrados por primera vez en la región Nororiental de Colombia, son importantes por cuanto se contara con materiales seleccionados disponibles para los cultivadores de mora de la región.

In this study plant materials were selected in 53 farms belonging to four growers associations of blackberry in the municipalities of Pamplona and Chitagá (North of de Santander, Colombia). Nodal segments were used as initial explants of R. glaucus. For the establishment stage Murashige and Skoog, 1962 (MS) media was used and, supplemented with of gibberellic acid (GA3) (0.0 -0.1 mg/L) and 6-aminopurine (BAP) (0.0 -2.0 mg/L); for the multiplication stage MS was supplemented with GA3 (0.0 -0.03 mg/L) and BAP, (0.0 -2.5 mg/L) and for the rooting stage MS was supplemented with acid indolbutirico (0.0 -1.0 mg/L). From the data generated during the three stages, an experimental design of incomplete blocks was randomly applied and the treatments averages were statistically analyzed using the Tukey Test. The results indicated average rates of contamination (16.5-49.7 %), multiplication (3.8-4.3 shoots/explant) and in vitro rooting (3.3-4.3 roots/plant) for the different evaluated materials. These results, achieved first in the Northeastern region of Colombia, are important in that they will feature selected materials available for blackberry growers in the region.

Pleasure Growers: Experiências e Produção de Sentido do Envelhecimento numa Rede Social Digital / Pleasure Growers: Experiences and Aging Sense Production in the Digital Social Network

O objetivo deste artigo é discutir a produção de sentido e as experiências estéticas sobre o envelhecimento na comunidade “Nova Cara da Terceira Idade”, em uma rede social digital, o Facebook. Uma análise dialógica, pautada segundo a perspectiva de Bakhtin (1997), foi a metodologia utilizada neste artigo. O objeto empírico se constitui de publicações digitais que apresentam de forma positivada aspectos da velhice, por meio da auto-representação de um grupo de idosos, denominados por Morace (2009) de pleasure growers. Sujeitos que redescobrem os valores das suas utopias juvenis, embasados pelas experiências e pela maturidade alcançada. Parte-se da premissa de que os julgamentos de valor acontecem nas interações verbais cotidianas, na constituição das significações postas pelos diálogos (Bakhtin, 1997), pelas experiências vivenciadas em coletividade. A estrutura da realidade do indivíduo, do “eu idoso” como um processo histórico e social. Como um dos resultados, verifica-se que idosos estão se apropriando, cada vez mais, das tecnologias de comunicação e informação para refratar as visões negativas da velhice...

The purpose of this article is to discuss the production of meaning and aesthetic experiences on aging in community "New Face of the Third Age," in a digital social network, Facebook. A dialogical analysis, based from the perspective of Bakhtin (1997), was the methodology used in this article. The empirical object constitutes of digital publications that present positively valued aspects of old age, through self-representation of a group of elderly, called by Morace (2009), pleasure of growers. Subjects who rediscover the values of their desires of youth, grounded by the experience and the achieved maturity. It starts with the premise that the value judgments happen in everyday verbal interactions, in the constitution of meanings made by dialogues (Bakhtin, 1997), by experiences in the community. The reality structure of the individual, the "old me" as a social and historical process. As a result, one realizes that the elderly are increasingly grabbing communication technologies and information to refract negative views of old age...

Efeito de probiótico na infecção e excreção fecal de Salmonella em suínos / Effect of probiotic on the Salmonella infection and fecal excretion in pigs

A transmissão de Salmonella na cadeia produtiva de suínos é um problema de difícil controle. O objetivo do estudo foi avaliar o efeito da administração oral de probiótico sobre a ocorrência de infecção e excreção fecal de Salmonella em suínos em fase de crescimento. Os tratamentos consistiram de ração basal sem aditivos (controle) ou adicionada de probiótico (10(7)ufc g-1 de células viáveis dos gêneros Bifidobacterium, Enterococcus, Lactobacillus e Saccharomyces). Foram alocados seis leitões de 50 dias em cada tratamento, com duas repetições por tratamento. Todos os animais foram inoculados com Salmonella Typhimurium (10(6)ufc mL-1) após 14 dias do alojamento. Semanalmente, foram coletadas amostras de sangue e fezes e no dia 35 pós-inoculação os animais foram sacrificados e necropsiados. Os animais de ambos os tratamentos foram infectados por Salmonella e soroconverteram. Não houve diferença (P>0,05) entre os grupos nas médias de Salmonella, Enterococcus, Lactobacillus e coliformes totais nas fezes, porém a administração de probiótico resultou em menor frequência de isolamento de Salmonella a partir de fígado (P=0,04), linfonodos mesentéricos (P=0,04), pulmão (P=0,03) e baço (P=0,01). Conclui-se que os microrganismos probióticos testados não foram capazes de impedir a infecção ou a excreção fecal de Salmonella em suínos de crescimento, mas diminuíram o número de portadores em linfonodos mesentéricos.

Control of Salmonella transmission has been a challenge for the pork production companies. The aim of this study was to evaluate the effect of oral administration of probiotics on the occurrence of infection and fecal excretion of Salmonella in growers. The treatments consisted of basal diet without additives (control) or added of probiotic (10(7)cfu g-1 of viable cells of the genera Bifidobacterium, Enterococcus, Lactobacillus and Saccharomyces). Six 50 days-old pigs were allocated into each treatment, with two replicates per treatment. All animals were inoculated with Salmonella Typhimurium (10(6)cfu mL-1) after 14 days of housing. Afterwards, blood and feces samples were taken weekly and on day 35 post-inoculation the animals were euthanized and necropsied. The animals in both treatment groups were infected by Salmonella and seroconverted. There was no difference (P>0.05) between groups in mean counts of Salmonella, Enterococcus, Lactobacillus and coliforms in the feces samples, but the probiotic administration resulted in a lower frequency of isolation of Salmonella from liver (P=0.04), mesenteric lymph nodes (P=0.04), lung (P=0.03) and spleen (P=0.01). It was concluded that the probiotic microorganisms tested in this study were not able to protect against the infection or to decrease the fecal excretion of Salmonella in growing pigs, but were able to decrease the number of carriers in the mesenteric lymph nodes.