RESUMEN
Objective To clarify the active ingredients of Guanxin Qiwei tablet, and to explore the potential target for its efficacy. Methods We studied the interaction between Chinese medicine ingredients in the Guanxin Qiwei tablet and the therapy targets using a network pharmacology model, which was developed by integrating oral bioavailability prediction, druggability analysis, principal component analysis, molecular docking simulation and network of pharmacophore-target-disease analysis. Results Eighteen principal compounds had high-degree network with 41 therapy targets for coronary heart disease (CHD), and had higher affinity with 18 potential targets via the calculation of molecular docking. Further analysis showed that 7 key targets were closely related to the treatment of CHD. Conclusion We have screened 18 possible active molecules in Guanxin Qiwei tablet and 7 key therapy targets, and preliminarily elucidate the molecular mechanism based on the “multi-ingredient, multi-target, and synergistic effect”.
RESUMEN
Objective To clarify the active ingredients of Guanxin Qiwei tablet,and to explore the potential target for its efficacy.Methods We studied the interaction between Chinese medicine ingredients in the Guanxin Qiwei tablet and the therapy targets using a network pharmacology model,which was developed by integrating oral bioavailability prediction,druggability analysis,principal component analysis,molecular docking simulation and network of pharmacophore-target-disease analysis.Results Eighteen principal compounds had high-degree network with 41 therapy targets for coronary heart disease (CHD),and had higher affinity with 18 potential targets via the calculation of molecular docking.Further analysis showed that 7 key targets were closely related to the treatment of CHD.Conclusion We have screened 18 possible active molecules in Guanxin Qiwei tablet and 7 key therapy targets,and preliminarily elucidate the molecular mechanism based on the multi-ingredient,multi-target,and synergistic effect.
RESUMEN
[Objective] To establish the quality standard for Guanxin Qiwei Tablet. [Methods] Radix Salviae Miltiorrhizae, Lignum Dalbergiae Odoriferae, Rhizoma Kaempferiae and Fructus Choerospondiatis in Guanxin Qiwei Tablet were identified by TLC. TanshinoneⅡA content was determined by HPLC.[Results] Radix Salviae Miltiorrhizae, Lignum Dalbergiae Odoriferae, Rhizoma Kaempferiae and Fructus Choerospondiatis can be identified by TLC, the spot being clear without the interference of negative control. A good linearity was in the range of 0.022-0.154 ?g, the average recovery of tanshinone ⅡA was 97.9%, and RSD was 1.22%. [Conclusion] This method is simple and can be used to evaluate the quality of Guanxin Qiwei Tablet.