Ginkgolide B inhibits the malignant biological behaviors of gastric cancer HGC-27 cells by blocking the PI3K/Akt/mTOR signaling pathway / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨银杏内酯B（GKB）是否通过阻抑PI3K/Akt/mTOR信号通路抑制胃癌HGC-27细胞的增殖、凋亡、迁移及侵袭。方法：将HGC-27细胞分为对照、GKB低剂量（100 mg/L）、GKB高剂量（200 mg/L）、GKB高剂量（200 mg/L）+740Y-P（PI3K激活剂）、Ly294002（PI3K抑制剂）组。采用MTT、Edu、FCM、Transwell实验分别检测各组细胞的增殖、凋亡、迁移和侵袭能力，qPCR和WB法分别检测各组细胞中PI3K mRNA、Akt mRNA、mTOR mRNA和Ki-67、caspase-3、p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR蛋白的表达。构建胃癌HGC-27细胞裸鼠移植瘤模型，观察GKB对移植瘤生长的影响，WB法检测移植瘤组织中Ki-67、caspase-3、p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR蛋白的表达。结果：体外实验结果表明，与对照组相比，GKB低剂量组、GKB高剂量组、Ly294002组HGC-27细胞的增殖活力及细胞增殖率、迁移和侵袭细胞数，PI3K、Akt、mTOR mRNA表达，以及Ki-67、p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR蛋白表达均显著降低（均P<0.05）；细胞凋亡率、caspase-3蛋白表达均显著升高（均P<0.05）；740Y-P可部分逆转GKB对HGC-27细胞的抑制作用（均P<0.05）。荷瘤裸鼠实验结果显示，GKB可显著抑制HGC-27细胞裸鼠移植瘤的生长（P<0.05），且可下调PI3K/Akt/mTOR通路相关蛋白的表达。结论：GKB可通过阻抑PI3K/Akt/mTOR信号通路而抑制胃癌HGC-27细胞增殖、迁移与侵袭并促进其凋亡。

Expression of mitogen-activated protein kinase kinase 1 in gastric cancer and its clinical significance / 解剖学报

Objective To investigate the expression of mitogen-activated protein kinase kinase 1 (MAP2K1) in gastric cancer and its clinical significance. Methods Immunohistochemistry and Western blotting were used to detect the protein expression of MAP2K1 in gastric cancertissues and cells. The morphology and the expression position of MAP2K1 were observed by immunofluorescence. MAP2K1 mRNA expression in gastric cancer tissues was analyzed by data mining of Starbase database and Oneomine database. The correlation between MAP2K1 mRNA expression and clinicopathological features was analyzed by UALCAN database. Survival analysis was performed using Kaplan Meier-Plotter online analysis tools. GEPIA2 database mining the relationship between MAP2K1 and gastric cancer stem cell related factors and drug resistance related factors. Results Immunohistochemistry, immunofluorescence and Western blotting showed that MAP2K1 protein was highly expressed in gastric cancer tissues and cells, and MAP2K1 was expressed in the cytoplasm of gastric cancer. According to the analysis of various databases, the expression of MAP2K1 mRNA in gastric cancer tissue was higher than that in normal gastric tissue, and the expression of MAP2K1 mRNA was closely related to gastric cancer stage, grade, lymph node metastasis and patient gender, and the overall survival rate of gastric cancer patients in the group with high MAP2K1 mRNA expression was significantly lower than that in the group with low MAP2K1 mRNA expression, which may be related to the characteristics of gastric cancer stem cells and drug resistance. Conclusion MAP2K1 is highly expressed in gastric cancer, and its expression level may affect the poor prognosis of patients by regulating stem cell related factors and drug resistance related factors. MAP2K1 may be a new diagnostic marker to determine the prognosis of gastric cancer patients.

Inhibitory Effect of Hederasaponin B on Gastric Cancer HGC-27 Cells and Its Mechanism Based on Bioinformatics / 中国实验方剂学杂志

ObjectiveTo investigate the inhibitory effect of hederasaponin B on gastric cancer HGC-27 cell and the mechanism. MethodMethyl thiazolyl tetrazolium （MTT） assay， hematoxylin-eosin （HE） staining， 4'，6-diamidino-2-phenylindote （DAPI） staining， colony formation assay， scratch assay， and flow cytometry were employed for the analysis of apoptosis and cell cycle. Thereby， the inhibitory effect of hederasaponin B on gastric cancer HGC-27 cell was investigated. Then the Pharm Mapper， UniProt， Swissdock， STRING， and Metascape were used for target screening， gene annotation， molecular docking， protein-protein interaction （PPI） network construction， Gene Ontology （GO） term and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis to explore the mechanism. ResultHederasaponin B （15， 30， 60， 120 μmol·L-1） can significantly reduce the survival rate of HGC-27 cell （P<0.01） in a time-dependent and dose-dependent manner compared with the blank group. It had no significant toxicity to normal GES-1 cell at concentration below 120 μmol·L-1. Compared with the blank group， hederasaponin B （30， 60， 120 μmol·L-1） induced cytoplasmic vacuolization， and nuclear deformation and karyopyknosis， inhibited the migration of HGC-27 cell （P<0.01）， and brought about the apoptosis （P<0.05， P<0.01） and cell cycle arrest of HGC-27 cell （P<0.05， P<0.01）. Hederasaponin B （10， 20， 30 μmol·L-1） also suppressed the independent survival ability and proliferation ability of HGC-27 cell （P<0.01）. The possible action targets were kinesin-like protein KIF11， cGMP-specific 3，5 cyclic phosphodiesterase， caspase-3， serine/threonine protein kinase Chk1， proto-oncogene tyrosine protein kinase， epidermal growth factor receptor， and mitogen-activated protein kinase （MAPK） 8. The mechanism may be related to MAPK signaling pathway （pathways in cancer）， adhesion connection， focal adhesion and proteoglycans in cancer （epithelial cell signaling pathways in Helicobacter pylori infection）. ConclusionHederasaponin B exerts significant inhibitory effect on gastric cancer HGC-27 cell through multiple targets and multiple pathways.

LINC01018 regulates the proliferation, apoptosis and radiosensitivity of gastric cancer HGC-27 cells through miR-297 / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨长基因间非编码RNA（long intergene non-coding RNA，LINC）01018是否通过抑制miR-297调控胃癌HGC-27细胞的增殖、凋亡及放射敏感性。方法： 收集于青海省第五人民医院接受手术的胃癌患者（21例）及化疗抵抗胃癌患者（19例）的癌组织和癌旁组织，以qPCR检测胃癌组织、化疗抵抗胃癌组织和胃癌HGC-27细胞中LINC01018、miR-297的表达。双荧光素酶报告基因实验验证LINC01018与miR-297之间的靶向关系。在HGC-27细胞中转染LINC01018过表达质粒pcDNA-LINC01018或miR-297抑制剂，或共转染pcDNA-LINC01018和miR-297模拟物，以qPCR检测验证细胞转染效果。MTT和克隆形成实验检测转染后HGC-27细胞的增殖活力与克隆形成能力，流式细胞术检测转染后HGC-27细胞的凋亡率，克隆形成实验检测各组转染后HGC-27细胞的放射敏感性，WB实验检测细胞中Ki67、cleaved-caspase3、pro-caspase3蛋白的表达。结果： 与癌旁组织或胃正常黏膜上皮细胞GES-1相比，胃癌组织、化疗抵抗胃癌组织和胃癌HGC-27细胞中LINC01018呈低表达、miR-297呈高表达（均P<0.01）。LINC01018与miR-297存在靶向结合关系，LINC01018负向调控miR-297的表达。过表达LINC01018或敲减miR-297可抑制HGC-27细胞的增殖活力与克隆形成能力、降低细胞存活率，促进细胞的凋亡、下调Ki67和pro-caspase3蛋白表达水平、上调cleaved-caspase3蛋白表达水平、提高HGC-27细胞的放射敏感性（均P<0.01）。共转染pcDNA-LINC01018和miR-297模拟物可逆转过表达LINC01018对HGC-27细胞的所有上述作用，尤其可逆转其对HGC-27细胞的放射增敏作用（P<0.05或P<0.01）。结论： LINC01018通过下调miR-297表达抑制胃癌HGC-27细胞增殖而促进凋亡和增强细胞的放射敏感性，该作用与Ki67和caspase3的表达变化有关。

Effects of microRNA-4286 on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type I / 解剖学报

Objective To investigate the effects of microRNA-4286 (miRNA-4286) on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type I (INPP4A). Methods HGC-27 cells were divided into blank control group, negative control group and intervention group. The blank control group received no treatment, negative control group was transfected with pEGFP-Nl plasmid, and intervention group was transfected with pEGFP-Nl-miRNA-4286 plasmid. Real-time PCR was used to detect the expression of miRNA-4286 in HGC-27 cells, and Western blotting was used to detect the expression of INPP4A protein in HGC-27 cells. The proliferation rate, invasion ability and migration ability of HGC-27 cells were detected by cholecystokinin octapeptide, cholecystokinin octapeptide (CCK8), Transwell chamber assay and scratch test, respectively. Results The miRNA-4286 expression level, proliferation rate, and number of transmembrane cells in intervention group were significantly higher than those in blank control group and negative control group (P0.05). Conclusion miRNA-4286 may promote the growth, migration and invasion of gastric cancer cell line HGC-27 by down- regulating the expression of INPP4A.

Ensayo de reproducción inducida en Ariopsis seemanni / Induced spawning trial with Ariopsis seemanni / Ensajo de reprodução induzida em Ariopsis seemanni

Resumen El bagre marino Ariopsis seemanni es aprovechado como pez de consumo en la alimentación de pescadores y pobladores de la región pacifica colombiana y como pez ornamental (5 a 10 cm -longitud total), a nivel comercial nacional e internacional. En Colombia esta especie ha sido priorizada en la agenda nacional de investigación en acuicultura de 2011-2012, como una de las especies marinas potenciales en actividades acuícolas. El objetivo de esta investigación fue evaluar el efecto de la gonadotropina coriónica humana (HGC) y el extracto pituitario de carpa (EPC) sobre la maduración final y el desove en hembras adultas de la especie. Se utilizaron 24 parejas de peces silvestres capturadas del medio, con un peso promedio de 263.6±42.2 g y 174.4±30.4 g para hembras y machos, respectivamente. Se utilizaron cuatro tratamientos hormonales: T1: 5 mg EPC/kg; T2: 7 mg EPC/kg; T3: 2000 UI HGC/kg y T4: 1000 UI HGC/kg. Se identificaron tres tipos de oocitos en maduración, no se presentaron diferencias estadísticamente significativas (p>0,05) entre los tratamientos utilizados y la cantidad de oocitos en maduración; no se obtuvo desove espontaneo en ninguna de las hembras inducidas con las dosis hormonales empleadas.

Abstract The tete sea catfish Ariopsis seemanni is a fish used for human consumption by fishermen and residents along the coast of Colombia and as ornamental fish (5 to 10 cm -total longitude), at national and international comercial level. In Colombia, this species has been prioritized in the national aquaculture research agenda 2011-2012, as one of the potential marine species for aquaculture activities. This research was aimed at evaluating the effect of human chorionic gonadotropin (HGC) and carp pituitary extract (EPC) on final maturation and spawning adult females of the species. 24 pairs of wild fish were used, with an average weight of 263.6±42.2 g and 174.4±30.4 g for females and males, respectively. Four hormonal treatments was be used: T1: EPC 5 mg/kg; T2: EPC 7 mg/kg; T3: HGC 2000 IU/kg and T4: HGC 1000 IU/kg. Identify three types of maturation oocytes was. There were no statistically significant differences (p>0,05) between treatments used and the number of oocytes achieving maturation. No spontaneous spawning was obtained in any of the females induced with the hormonal doses used.

Resumo O bagre marino Ariopsis seemanni é aproveitado como peixe de consumo na alimentação de pescadores e moradores da região pacifica colombiana e como peixe ornamental (5 a 10 cm de comprimento), a nível comercial nacional e internacional. Na Colômbia A. seemanni tem sido priorizado na agenda nacional de pesquisa na aquicultura de 2011-2012, como uma das espécies marinhas potenciais em atividades aquícolas. Esta pesquisa teve como objetivo avaliar o efeito da gonadotrofina coriónica humana (GCH) e o extrato pituitário de carpa (EPC) sobre a maduração final e o desove em fêmeas adultas desta espécie. Utilizaram-se 24 casais de peixes retirados do meio natural, com um peso médio de 263,6 ± 42,2 g e 174,4 ± 30,4 g para fêmeas e machos, respectivamente. Empregaram-se quatro tratamentos hormonais: T1: 5 mg EPC/kg; T2: 7 mg EPC/kg; T3: 2000 UI HGC/kg e T4: 1000 UI HGC/kg. Identificaram-se três tipos de oócitos em maduração, não houve diferencias estatisticamente significativas (p>0,05) entre os tratamentos utilizados e a quantidade de oócitos em maturação; não foi possível obter desove espontâneo em nenhuma das fêmeas induzidas com as doses hormonais empregadas.

In Vitro Antitumor Bioactivity and GC-MS Analysis of Petroleum Ether Fraction of Boehmeria nivea Root / 中国实验方剂学杂志

Objective:To define the anti-gastric cancer activity in vitro of petroleum ether fraction of Boehmeria nivea root and reveal the material basis of its efficacy, so as to lay the foundation for the development and utilization of B. nivea root. Method:Methyl thiazolyl tetraolium(MTT) method was used to evaluate the inhibitory rate and time-dose relationship of petroleum ether fraction of B. nivea root with different doses and delivery times on human gastric cancer HGC-27 cells. Flow cytometry was used to detect the change of cell apoptosis and cell cycle after petroleum ether fraction of B. nivea root acted on human gastric cancer HGC-27 cells. GC-MS was used to detect the components of petroleum ether fraction of B. nivea root. Result:Experiment data showed significant cell proliferation inhibition in an obvious time-dose-effect manner, with statistically significant differences (PB. nivea root. The effect of petroleum ether fraction of B. nivea root on human gastric cancer HGC-27 cells could induce apoptosis,which affects the normal changes of cell cycle. The percentage of cells was decreased significantly in G0/G1 phase,and that in S phase was significantly increased. GC-MS was used to identify 26 chemical constituents in petroleum ether of B. nivea root,including sitosterol and stigmasterol. Conclusion:Petroleum ether fraction of B. nivea root is the active anti-gastric cancer part,and its main effective component is sterol compounds. This lays the foundation for the rational application of B. nivea root in clinic and the further research in tis anti-tumor effect.

Aloin induces apoptosis regulating the activation of MAPKs signaling pathway in human gastric cancer cells / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of aloin on apoptosis of human gastric cancer cells and explore the molecular mechanism.</p><p><b>METHODS</b>Gastric cancer MKN-28 and HGC-27 cells were cultured routinely in 1640 medium supplemented with 10% fetal bovine serum and 10% non-essential amino acids (for HGC-27 cells) and treated with different concentrations of aloin for different durations. The cell viability, cell nuclear morphology, and apoptotic rate of the cells were detected using CCK-8 assay, DAPI staining and AnnexinV-FITC/PI, respectively; Western blotting was used to detect the expression levels of PARP, procaspase 3 and the phosphorylation of p38, ERK and JNK. The cells were treated with specific inhibitors of p38, ERK and JNK, and the inhibitory effects on these pathways were detected with Western blotting; DAPI staining was used to detect the effects of inhibitors on apoptosis of gastric cancer cells.</p><p><b>RESULTS</b>Aloin dose-dependently inhibited the viability and induced apoptosis of HGC-27 and MKN-28 cells. Alion treatment obvious enhanced the phosphorylation of p38 and JNK but decreased ERK phosphorylation in the cells. Blocking ERK activation with the ERK inhibitor obviously enhanced aloin-induced cell apoptosis, where inhibiting p38 and JNK activation partly reversed alion-induced apoptosis in the cells.</p><p><b>CONCLUSIONS</b>Aloin induces apoptosis of human gastric cancer cells by activating p38 and JNK signaling pathways and inhibiting ERK signaling pathway.</p>

Inconsistent effect of chloroquine on apoptosis of normal gastric epithelial cells GES-1 and gastric cancer cell line HGC-27 / 中国免疫学杂志

Objective:To compare the effect of chloroquine on apoptosis of normal gastric epithelial cells and gastric cancer cell line HGC-27. Methods:Change of these two kinds of cells were observed by inverted microscope after treating with CQ. HGC-27 cells were detected on the effect of apoptosis by DAPI nuclear staining after treating with CQ. The proliferation of cells were measured by CCK-8. Changes of mitochondrial membrane potential were investigated by JC-1 after treating with CQ. The expression of apoptosis protein effector enzyme Caspase-3 and substrate PARP in these two kinds of cells were tested by Western blot after using chloroquine (CQ) and rapamycin ( rapamycin, RAP ) to treat cells 72 h. Results: After treated with 10 μmol/L CQ 72 h, morphological characteristics of GES-1 cells and HGC-27 cells could be visible under the microscope,CQ induced apoptosis of GES-1 cells,on the contrary,it could make the HGC-27 cell get widened,the number of apoptotic cells gradually increased,the cell density decreased,cell atrophy and gradually turned round,cytoplasm reduced,at last,lose normal cell morphology. After two kinds of cells treated with CQ 72 h,as for GES-1 cell nuclei stained light,nuclear size and shape were not changed,however,HGC-27 nuclei showed pyknosis or granular fluorescence dense concentrated form. CCK-8 results showed that comparing with normal gastric epithelial cells GES-1,the pro-liferation of gastric cancer HGC-27 cells activity could be inhibited by CQ. JC-1 results showed that the change of the red fluorescence to green fluorescence in HGC-27 cells treated by CQ. Western blot showed that after being treated with CQ and RAP in normal gastric epithelial cells and HGC-27 cell line 72 h,the expression of apoptosis protein Caspase-3 and PARP in gastric cancer cell HGC-27 decreased significantly,comparing to that in GES-1 cells. Conclusion:Compared to normal gastric epithelial cells,CQ can inhibit human gastric cancer HGC-27 cell viability and induce apoptosis.

Trametenolic acid B induces the programmed death of human gastric cancer HGC-27 cells / 中成药

AIM To study the programmed death effect of trametenolic acid B (TAB) against human gastric cancer HGC-27 cells.METHODS The proliferation of cells was examined by MTT assay;cell apoptosis rate and cell cycle were detected by flow cytometry;cell autophagy was observed by acridine orange staining and transmission electron microscope;expressions of autophagy-related proteins were detected by Western blot.RESULTS 10,20,40 μmol/L Trametenolic acid B had good growth-inhibitory effects on HGC-27 cells,blocked in G0/G1 phase,in a dose-dependent and time-dependent manner,but had no obvious effect on cell apoptosis.After treatment with 20 μmol/L trametenolic acid B for 24 h,a large number of autophagy vacuoles and autophagy bodies were observed under transmission electron microscope,and the proportion of cell autophagy was significantly increased with higher dose of TAB;TAB promoted the transformation of type Ⅰ microtubule-associated protein 1 light chain 3 (LC3 Ⅰ) to type Ⅱ microtubule-associated protein 1 light chain 3 (LC3 Ⅱ),up-regulated the expressions of myosin-like B-cell lymphoma-2 (Bcl-2) interacting protein (Beclin-1) and cyclin-dependent kinase inhibitor 1A (p21),and inhibited the CCND1 gene protein (CyclinD1) expression.CONCLUSION Trametenolic acid B can induce the autophagic death of HGC-27 cells,but it has no significant effect on cell apoptosis.

Preliminary research on the effect of elemene liposome on gastric cancer cell HGC-27 and its mechanism / 中国癌症杂志

Background and purpose:In recent years, many studies have showed that elemene liposome is widely used in the treatment of digestive tract tumors, malignant pleural effusion and ascites. This study combined in vitro and in vivoexperiments to observe the inhibitory effect of elemene liposome on the growth of human gastric cancer and the HGC-27 cell line.Methods:In order to screen the optimum concentration of elemene liposome, machine vision automatic live cell observation analysis system (Cell-IQ) was applied to detect the best inhibitory effect on human gastric cancer cell line HGC-27, and the lfow cytometry was applied to further detect the apoptosis of HGC-27 treated with elemene liposome. The model of human gastric cancer peritoneal metastasis in nude mice was established to investigate the intervention of elemene liposome and cisplatin (DDP) on peritoneal cancer index (PCI). CD31 marker of tumor microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) in tumor tissues were investigated to explore the mechanism underlying the inhibitory effect on peritoneal metastasis of HGC-27 cells in nude mice.Results:Cell-IQ analysis showed that the inhibitory effect of elemene liposome on HGC-27 presented in a positive concentration-dependent manner, which could not be further enhanced when the concentration exceeded 100 μg/mL with the best reaction time between 4 and 19 hours. Flow cytometry showed that the early apoptosis rate was 45% in the elemene liposome group and only 0.019% in the control group. The early apoptosis rate was signiifcantly higher in treatment group than that in control group (P0.05).Conclusion:Elemene liposome can inhibit human gastric cancer cells at the optimal concentration of 100 μg/mL with the best reaction time between 4-19 hours. Elemene liposome has a clear preventive effect on peritoneal metastasis of human gastric cancer in nude mice. Induction of apoptosis in gastric cancer cells may be the main mechanism underlying the inhibitory effect of elemene liposome on human gastric cancer cells.

The saponin DT-13 inhibits gastric cancer cell migration through down-regulation of CCR5-CCL5 axis / 中国天然药物

AIM@#To investigate the effect of DT-13 on gastric cancer cell migration, and to explore the possible mechanisms underlying the anti-metastasis activity of DT-13.@*METHODS@#Growth inhibition of DT-13 was analyzed by the MTT assay. Cell migration was measured by the scratch-wound assay and transwell double chamber assay. To investigate the possible mechanisms underlying the anti-metastasis activity of DT-13, chemokine receptors that are involved in cancer metastasis (CCR2, CCR5, CCR7, CXCR4, and CXCR6) were detected by conventional PCR. The effect of DT-13 on CCR5 and CXCR4 expression was further evaluated by quantitative PCR and Western blot, respectively. The secretion of CCL5 (ligand of CCR5) and SDF-1 (ligand of CXCR4) were detected by enzyme-linked immunosorbent assay (ELISA).@*RESULTS@#DT-13 inhibited BGC-823 and HGC-27 cell growth in a dose dependent manner, and the estimated IC50 value for 24 h treatment was 23.5 ± 5.1 μmol·L(-1) for BGC-823 cells and 35.6 ± 7.6 μmol·L(-1) for HGC-27 cells. DT-13 also significantly decreased gastric cancer cell migration. DT-13 significantly decreased the gene expression of CCR5 in both BGC-823 and HGC-27 gastric cancer cells, and moderately reduced the expression of CXCR4. Similar to the results of gene expression, significant down-regulation of CCR5 protein was observed, but CXCR4 protein levels were much less affected. CCL5 secretion, but not SDF-1 production, was inhibited by DT-13.@*CONCLUSION@#DT-13 inhibited gastric cancer cell migration by down-regulation of the CCR5-CCL5 axis.

Assessment of Collagen-Induced Arthritis Using Cyanine 5.5 Conjugated with Hydrophobically Modified Glycol Chitosan Nanoparticles: Correlation with 18F-Fluorodeoxyglucose Positron Emission Tomography Data

OBJECTIVE: To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). MATERIALS AND METHODS: We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 microL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice. RESULTS: The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 +/- 0.76 and 0.79 +/- 0.71, respectively, for CIA mice; and 0.57 +/- 0.17 and 0.54 +/- 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 +/- 1.54 x 10(5) and 2.75 +/- 1.51 x 10(5), respectively, for CIA mice, and 1.22 +/- 0.27 x 10(5) and 0.88 +/- 0.24 x 10(5), respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice. CONCLUSION: NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.

Photodynamic efficiency of new porphyrin-typed drug on HGC27 and MGC803 cells / 国际生物医学工程杂志

Objective To explore photodynamic therapeutic efficiency and related mechanisms of a new porphyrin-typed photodynamic therapy (PDT) drug synthesized in our lab on HGC27 and MGC803 cells.Methods Two different kinds of gastric cancer cells, HGC27 and MGC803, were chosen as cell lines to evaluate the PDT efficiency of new porphyrin-typed PDT drug based upon four group experiments: control (no drug, no light) group, drug treated group (only drug, no light), light treated group (no drug, only light) and experiment group (with drug and light at the same time). Bleaching method was used to evaluate the photostability of new porphyrin-typed PDT drug upon repititive illumination. MTT assay was carried out to test the survival rate of tumor cells after PDT. Cofocal laser scanning microscope (CLSM) was applied to observe subcellular localization of drug in HGC27 and MGC803 cells (mitochondria and lysosome). Results New porphyrin-typed PDT drug has good stability to light, no toxicity on HGC27 and MGC803 cells without light (P>0.05), while intense lethal effect was observed upon light illumination (P<0.05). The peak efficacy appeared when concentration is 3.12 μmol/L for both HGC27 and MGC803 cells and the new porphyrin-typed PDT drug localizes in lysosome other than traditional mitochondrion. Conclusion New porphyrin-typed PDT drug is a good photodynamic therapeutic sensitizer for HGC27 and MGC803 tumor cells.