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Journal of the Korean Gastric Cancer Association ; : 19-25, 2003.
Artículo en Coreano | WPRIM | ID: wpr-88533

RESUMEN

PURPOSE: Dysregulation of apoptosis may attribute to development of cancer by abnormally prolonging cell viability with accumulation of transforming mutations. Survivin and HIAP (Human Inhibitors of Apoptosis)-1 were recently described as apoptosis inhibitors. Their pathogenic roles in gastric cancer are largely unknown. In the present study, we examined the expression of survivin and HIAP-1 in gastric cancer tissues and cell lines in order to elucidate the roles of survivin and HIAP-1 in the process of gastric carcinogenesis. MATENRIALS AND METHODS: Eight gastric cancer cell lines and five gastric cancer tissues were studied. The expression of survivin and HIAP-1 were evaluated by reverse transcription -polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot. RESULTS: Western blot and RT-PCR analysis revealed survivin and HIAP-1 expression in all gastric cancer cell lines. Increased expression of survivin and HIAP-1 were found in all cases of gastric cancer tissues compared to normal tissues by Western blot analysis. In immunohistochemical analysis tumor cells were stained with anti-survivin and anti-HIAP-1 antibodies. Cell cycle dependence of survivin expression was preserved in gastric cancer cell lines. CONCLUSION: The results indicate that increased expression of survivin and HIAP-1 genes may play an important role in gastric cancer.


Asunto(s)
Anticuerpos , Apoptosis , Western Blotting , Carcinogénesis , Ciclo Celular , Línea Celular , Supervivencia Celular , Inmunohistoquímica , Transcripción Reversa , Neoplasias Gástricas
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