Identification bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia with antimicrobial activity against human pathogens

Aims@#Antimicrobial compounds are bioactive compounds that have ability to inhibit microbial growth activities. This study aimed to screen and identify bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia that had potential to produce antimicrobial compounds against Escherichia coli, Candida albicans and Staphylococcus epidermidis.@*Methodology and results@#The method used to screen and identify bacteria in this study including screening assay, morphological identification, Gram staining and spore staining method, biochemical tests and molecular identification based on 16S rRNA gene. This study resulted 16 isolates which were successfully isolated from Haliclona sp. According to screening assay, 5 isolates could potentially produce antimicrobial compounds coded as HEBS1, HEBS3, HEBS6, HEBB2 and HEBB3. Based on Gram staining, spore staining, biochemical test and molecular identification results, HEBS1 had proximity to Brachybacterium paraconglomeratum, HEBS3 had proximity to Kocuria palustris, HEBS6 had proximity to Psychrobacter pasificensis, HEBB2 had proximity to Bacillus aryabhattai, and HEBB3 had proximity to Bacillus toyonensis.@*Conclusion, significance and impact of study@#From 16 isolates that successfully isolated, there were 5 isolates that could potentially produce antimicrobial compounds against Escherichia coli, Staphylococcus epidermidis and Candida albicans. These isolates can be served as antimicrobial compounds producer. However, identification and purification of these antimicrobial compounds are needed to be done before applied it for medicine in the future.

Antigout effect of renierol extracted from the marine sponge in the South China Sea / 中国海洋药物

Objective To investigate the inhibitive effect of renierol extracted from the marine sponge in the South China Sea on xanthine oxidase and the effect on mice hyperuricemia as well as its possible mechanisms.Methods Renierol was extracted from sponge and its antigoat effect was tested.The activity of xanthine oxidase was measured by using the generation of uric acid method and the NBT reduction induced by superoxide anions,then its possible mechanism was estimated.Results The two methods showed renierol could obviously inhibit the effect of xanthine oxidase with IC_(50)of 1.85 and 1.36?g?mL~(-1),respectively.Conclusion Renierol extracted from the marine sponge can effectively inhibit the effect of xanthine oxidase.

STUDIES ON THE CHEMICAL CONSTITUENTS OF THE MARINE SPONGE HALICLONA SP / 中国海洋药物

The chemical constituents of the marine sponge Haliclona sp. collected from the South China Sea have been studied. An alkanoid 1 and an aryl acid 2 were isolated from the marine sponge and the structure of 1 and 2 were respectively elucidated as 1,3 -dimethylxanthine and p - hydroxybenzoic acid by spectroscopic analysis and elemental analysis.