Application of active films with natural extract for beef hamburger preservation / Aplicação de filmes ativos com extrato natural para preservação de hambúrguer de carne bovina

ABSTRACT: Edible chitosan-based films, in pure form (CH100), or blended with gelatin (GEL50:CH50), with and without boldo extract (B) were prepared. The oxygen permeability of the films, as well as color changes and antioxidant and antimicrobial activities on beef hamburgers samples were evaluated. CH100+B film exhibited optimal oxygen barrier properties (6.40±0.170cc-mL/[m²-day]), and this value was significantly increased in blended films (GEL50:CH50+B = 12.99±1.50cc-mL/[m²-day]) (P<0.05). All films provoked color changes in hamburger samples during storage, and the samples covered with CH100+B film showed a lower brightness and a higher redness. All films effectively protected beef hamburger from lipid oxidation, and boldo extract incorporation in pure and blended films enabled significant protection, when compared to the control sample (155.78±0.85meq O2/kg) (P<0.05). Finally, all films proved to be effective in microbial growth control, suggesting that these films are an effective alternative in improving food safety and quality of fresh meat.

RESUMO: Filmes comestíveis à base de quitosana, na forma pura (CH100), ou misturados com gelatina (GEL50:CH50), com e sem extrato de boldo (B), foram preparados. Avaliou-se a permeabilidade ao oxigênio dos filmes, assim como mudanças de cor e atividades antioxidantes e antimicrobianas sob amostras de hambúrguer de carne bovina. Filmes de CH100+B exibiram ótimas propriedades de barreira ao oxigênio (6,40±0,170cc-mL/[m²-dia]), e este valor foi significativamente aumentado em filmes mistos (GEL50:CH50+B = 12,99±1,50cc-mL/[m²-dia]) (P<0,05). Todos os filmes provocaram mudanças de cor nas amostras de hambúrgueres durante o armazenamento, apresentando as amostras cobertas com o filme CH100+B uma menor luminosidade e um maior vermelhidão. Todos os filmes protegeram efetivamente o hambúrguer de carne bovina da oxidação lipídica, e a incorporação do extrato de boldo em filmes puros e misturados possibilitou significativa proteção, quando comparada à amostra controle (155,78±0,85 meq O2/kg) (P<0,05). Finalmente, todos os filmes mostraram-se eficazes no controle do crescimento microbiano, sugerindo que estes filmes são uma alternativa eficaz para melhorar a segurança dos alimentos e a qualidade da carne fresca.

Natural antioxidant from yerba maté (Ilex paraguariensis St. Hil.) prevents hamburger peroxidation

The aim of this study was to evaluate whether yerba maté alcoholic extracts at very low concentrations (0.01 and 0.1 percent), prevent/retard lipid peroxidation in beef hamburgers without impairing sensory acceptability. For this TBARs and hexanal levels, fatty acid profile and cholesterol oxides were evaluated as oxidation parameters in beef hamburgers during 90 days' storage. The addition of 0.01 percent yerba maté ethanolic extracts proved inefficient in restraining the lipid peroxidation while the addition of 0.1 percent resulted in efficient antioxidant activity. Sensory evaluation of hamburger containing 0.1 percent yerba maté ethanolic extracts showed good acceptability. Yerba maté ethanolic extracts could entirely or partially replace the phenolic synthetic antioxidants in beef hamburgers only when used at above legally allowed concentrations for antioxidant additives (0.01 percent).

Processamento e características de qualidade de hambúrguer de carne de jacaré-do-pantanal (Caiman crocodillus yacare)

Objetivou-se neste trabalho desenvolver cinco formulações de hambúrguer de carne de jacaré-do-pantanal (Caiman crocodillusyacare) com variações no teor de gordura e na adição de fumaça líquida, como forma de agregar valor na cadeia dederivados cárneos desta espécie. As amostras foram processadas a partir de aparas de carne de jacaré-do-pantanal,obtidas de frigorífico sob o Serviço de Inspeção Federal. Foram produzidos 35kg de hambúrgueres com peso médio de 80g,imediatamente embalados a vácuo e submetidos a processo de congelamento rápido a 18oC±1oC. Para o controle físicoquímicoforam realizadas análises de umidade, proteína, gordura, cinzas, pH, atividade de água (Aa) e valor calórico. Nocontrole bacteriológico dos hambúrgueres foram realizadas contagem de bactérias heterotróficas aeróbias mesófilas,contagem de bactérias heterotróficas aeróbias psicrotróficas, enumeração (determinação do número mais provável) decoliforme total e fecal, contagem e identificação de Staphylococcus coagulase positiva e pesquisa de Salmonella spp. Doponto de vista físico-químico, a melhor formulação, com baixo valor calórico, foi 5% de adição de gordura e adição de fumaça,com possibilidade real para o desenvolvimento industrial. Nas análises de bactérias heterotróficas aeróbias mesófilas epsicrotróficas, coliformes totais e fecais, Staphylococcus coagulase positiva e Salmonella spp., todas as amost

Recognition and use of Nutrition Labeling among Hamburger and Pizza Restaurant Consumers in Daejeon / 대한지역사회영양학회지

To investigate recognition and use of nutrition labeling on eating-out menus, self-recorded questionnaire was surveyed for 641 consumers who visited hamburger and pizza restaurants in Daejeon from May to July, 2010. Subjects were women of 51.3% and 92.9% aged 10s or 20s. Use frequency of hamburger and pizza restaurants was 2-3 times a month with 56.9%. Of subjects 41.0% knew nutrition labeling mandates of eating-out menus, 64.6% have seen nutrition labels of menus, and 44.5% read nutrition labels when purchasing. Among subjects who read nutrition labels, 67.0% (29.8% of total) referred to it when menu purchase and 60.4% (26.9% of total) experienced changing menu. The ratio of reading nutrition labels was different among gender, diet trial frequency and monthly eating-out cost. Reasons for not reading labels were "not interested in" or "never heard about" for 57.0%, and "not noticeable" for 28.7%. The ratio who read correctly all 3 pieces of information, such as calorie amount, daily value, and way of using daily value was 39.2%. Average number of correct reading was 2.13, which was different among gender, marital status, having children, diet trial frequency, monthly eating-out cost, the restaurants use frequency, and accompanying person. Many subjects wanted to be additionally provided with cholesterol and transfat. Therefore, practical and differentiated consumer educations considering consumer features are required for better nutrition labeling usage. Government's regulatory efforts and voluntary effort of eating-out companies, such as adding nutrients on labels and improving labeling places, are also necessary.

Modelo de contaminación cruzada por Escherichia coli verocitotoxigénica durante la elaboración de hamburguesas caseras y evaluación cuantitativa de riesgos / Quantitative risk model for verocytotoxigenic Escherichia coli cross-contamination during hamburger preparation

El objetivo del presente trabajo fue generar un modelo probabilístico para evaluar cuantitativamente el riesgo de contaminación cruzada de E. coli verocitotoxigénica (VTEC) durante el proceso de elaboración de hamburguesas caseras y su impacto en la salud pública. El modelo tuvo en cuenta un grupo de prácticas culinarias corrientes y a cada una de ellas se le asignó la probabilidad asociada de transferencia de VTEC entre los alimentos y los utensilios de cocina. Las distribuciones de probabilidad que mejor describieron cada paso del proceso fueron incorporadas en el programa @Risk® y se realizaron las simulaciones empleando el análisis Monte Carlo. La manipulación de alimentos crudos (en este caso, la carne picada) antes de la preparación de alimentos que no demandan cocción (como las guarniciones de vegetales frescos que suelen acompañarlas) (Odds ratio, OR = 6,57), así como el hábito del lavado de manos (OR = 12,02) y de las tablas que se utilizan durante la elaboración de estos platos (OR = 5,02), fueron los principales factores de riesgo de contaminación cruzada del patógeno entre la carne y las verduras. La información aportada por este modelo debería considerarse durante el diseño de estrategias de comunicación del riesgo del síndrome urémico hemolítico para acentuar la importancia que estos factores pueden tener en la transmisión de la enfermedad.

The objective of this study was to develop a quantitative risk model for verocytotoxigenic Escherichia coli (VTEC) cross-contamination during hamburger preparation at home. Published scientific information about the disease was considered for the elaboration of the model, which included a number of routines performed during food preparation in kitchens. The associated probabilities of bacterial transference between food items and kitchen utensils which best described each stage of the process were incorporated into the model by using @Risk® software. Handling raw meat before preparing ready-to-eat foods (Odds ratio, OR, 6.57), as well as hand (OR = 12.02) and cutting board (OR = 5.02) washing habits were the major risk factors of VTEC cross-contamination from meat to vegetables. The information provided by this model should be considered when designing public information campaigns on hemolytic uremic syndrome risk directed to food handlers, in order to stress the importance of the above mentioned factors in disease transmission.

Hábitos de consumo de hamburguesas y riesgo de exposición a Escherichia coli verotoxigénica (VTEC): modelo de simulación / Hamburger consumption patterns and exposure assessment for verocytotoxigenic Escherichia coli (VTEC): simulation model

El objetivo del trabajo fue evaluar cuantitativamente el riesgo para la salud pública debido a la infección por VTEC derivado del consumo de hamburguesas. Mediante un modelo de simulación, se analizaron procesos de distribución, almacenamiento y venta, así como hábitos de consumo. La prevalencia y concentración de VTEC fue incluida en el modelo sobre la base de información científica publicada acerca de la enfermedad. Las distribuciones de probabilidad que mejor describieron cada paso del proceso fueron incorporadas en el programa @RiskR, y se realizaron múltiples simulaciones empleando el análisis Monte Carlo. El riesgo estimado de padecer la infección por VTEC en los adultos fue de 4,45 x 10-4; mientras que en los niños, los riesgos de adquirir la infección, de padecer Síndrome Urémico Hemolítico (SUH) y de mortalidad fueron de 2,6 x 10-4, 1,38 x 10-5 y 4,54 x 10-7, respectivamente. El riesgo de adquirir la infección y sus secuelas estuvo correlacionado con la concentración bacteriana en la carne (r = 0,664). El consumo de hamburguesas de elaboración propia (r = -0,203) estuvo asociado con el riesgo de enfermar dadas las características del almacenamiento (r = -0,567), que forman parte de los hábitos de consumo de la población. La información generada debería considerarse durante el diseño de estrategias de gestión y comunicación del riesgo del SUH, con énfasis en la importancia que estos factores tienen en la trasmisión de la enfermedad.

A quantitative risk assessment was developed for verocytotoxigenic Escherichia coli (VTEC) associated with hamburger consumption. The assessment (simulation model) considers the distribution, storage and consumption patterns of hamburgers. The prevalence and concentration of VTEC were modelled at various stages along the agri-food beef production system using input derived from Argentinean data, whenever possible. The model predicted an infection risk of 4.45 x 10-4 per meal for adults. The risk values obtained for children were 2.6 x 10-4, 1.38 x 10-5 and 4.54 x10-7 for infection, Hemolytic Uremic Syndrome (HUS) and mortality, respectively. The risk of infection and HUS was positively correlated with bacterial concentration in meat (r = 0.664). There was a negative association between homemade hamburgers (r = -0.116) and the risk of illness; however this association has been considered due to differences between retail and domiciliary storage systems (r = -0.567) and not because of the intrinsic characteristics of the product. The most sensitive points of the production system were identified through the risk assessment, therefore, these can be utilized as a basis to apply different risk management policies in public health.

Caracterização físico-química e sensorial de hambúrguer vegetal elaborado à base de caju / Physical chemical and sensory characterization of vegetal hamburger elaborated from cashew apple

A elaboração de produtos derivados do caju é uma alternativa para seu aproveitamento e para o consumo fora o período de safra. Objetivou-se neste trabalho caracterizar hambúrgueres elaborados à base de caju e comparar suas características com as de produtos comerciais. Foram adquiridos quatro tipos de hambúrgueres comerciais, um deles à base de carne e os demais à base de proteína vegetal. Os hambúrgueres foram analisados quanto às características físico-químicas (proteína, gordura, umidade, cinzas, carboidratos, atividade de água e pH) e aceitação sensorial (aparência, aroma, sabor, textura e impressão global). Foram observadas grandes variações nas características dos hambúrgueres: proteínas variaram de 5,75 a 12,95 por cento, gordura de 5,79 a 16,44 por cento, umidade de 45,83 a 63,71 por cento, cinzas de 2,89 a 4,75 por cento, carboidratos de 16,35 a 33,99 por cento, atividade de água de 0,958 a 0,983 e pH de 4,75 a 6,78. Os hambúrgueres de caju apresentaram menor pH e menores teores de proteína e gordura do que a maioria dos produtos comerciais. A aceitação sensorial também apresentou grandes variações, com notas para aparência de 6,2 a 7,8, aroma de 6,1 a 7,9, sabor de 5,7 a 8,0, textura de 5,9 a 7,9 e impressão global de 5,9 a 7,9. Para o hambúrguer de caju, a aceitação sensorial foi, em média, próxima da avaliação gostei ligeiramente (nota 6,0), para todos os atributos avaliados.

Products elaborated from cashew are good alternatives for their utilization and for consumption after harvest period. This work aimed to characterize hamburgers made from cashew apple and to compare their characteristics with the ones from commercial products. Four kinds of commercial products were bought, one made of meat and the others made of vegetal protein. Hamburgers were analyzed for their physical chemical characteristics (protein, fat, moisture, ash, carbohydrates, water activity and pH) and sensory acceptance (appearance, flavor, taste, texture and overall acceptance). Great variations were observed among hamburgers characteristics: proteins from 5.75 to 12.95 percent, fat from 5.79 to 16.44 percent, moisture from 45.83 to 63.71 percent, ash from 2.89 to 4.75 percent, carbohydrates from 16.35 to 33.99 percent, water activity from 0.958 to 0.983 and pH from 4.75 to 6.78. Cashew hamburgers showed less pH, protein and fat content than the major commercial products. Great variations were also observed for sensory acceptance, being within 6.2 to 7.8 for appearance, 6.1 to 7.9 for flavor, 5.7 to 8.0 for taste, 5.9 to 7.9 for texture and 5.9 to 7.9 for overall acceptance. Average sensory notes were nearly like slightly (note 6.0) for all analyzed attributes of cashew hamburger.