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Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate,The in- hibiton of cell proliferation,cell cycle distribution,expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay,flow cytometry,immunocytochemistry,and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24,48,72 h after intervention were 2.41?0.48,0.72?0.08,and 0.39?0.04 ug/ml,The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle,enhancement of Caspase-3,and inhibition of Survivin.
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Objective To observe the chemotactic role on umbilical vein endothelial cells of SMMC7721 hepatic(carcinoma) cells with angiopoietin gene expression in order to study the effects of angiopoietin on hepatocellular carcinoma angiogenesis.Methods Angiopoietin gene 1(Ang-1) fragment and Ang-2 fragment was transfected into SMMC7721 liver carcinoma cell line by Lipofectamine induced gene transfection technique.The chemotactic role of SMMC7721 liver carcinoma cell line on umbilical vein endothelial cells was observed through microchemotaxis analysis.Results The chemotactic response of the Umbilical vein endothelial cells was obviously improved with Ang-1 expression (P0.05).Conclusion Ang-1 is a chemotactic factor for vascular endothelial cell and a promoter for angiogenesis,whereas Ang2 does not show obvious chemotactic role.
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Objective:To prepare lactosaminated matrine liposomes and to study its targetability to liver in mice and efficacy of anti-tumor activities on human hepatoma cell line HepG2 in vitro.Methods:The matrine liposomes were prepared using the reverse-phase evaporation-ultrasonic technique.Lactosylphosphatidy-lethanolamine(Lac-PE) was synthesized and used for modified matrine liposomes..the diameter and entrapment efficiency of it were determined.The RP-HPLC was used for the determination of matrine concentration in mice tissue.The cytotoxic effect of LML on human hepatoma cell line HepG2 in vitro was detected by thiazolyl blue(MTT)assay.Results:The LML were nice and uniform,the particle diameter was between 80 and 150 nm and envelopment rats was 48.1%.Compared with matrine solution,ML and LML exhibited long circulation time.Tissue distribution results proved that the area under curve of liver was significantly difference among modified matrine liposomes,regular matrine liposomes and matrine solutions(P
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Objective To prepare cytokine-induced killer(CIK) in vitro, and study the biological activities of CIK. Methods Lymphocytes were isolated from peripheral blood and umbilical blood, and induced by cytokine.The cell surface markers CD_3 and CD_~56 of CIK were analyzed by FACS. The proliferation of CIK was tested using 3H -TdR release method, and the cytotoxic effect of CIK on hepatic carcinoma cells was measured by MTT. Results CIK cells quickly proliferated from the second week, and expanded more than 60-fold at the 31th day after induction. The CD_3+ and CD_~56 + cells expanded more than 800 folds. The cytotoxic effect of CIK on hepatic carcinoma cells was obviously stronger than that of LAK cells, and CIK had not obvious cytotoxic effect on fetal liver cells. Conclusion CIK was a highly efficient cytotoxic cells against tumors, and had clinical application potentials.
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The synergistic cytotoxicity of rhTNF-? or/and six chemotherapeutic agents namely ADM, MMC, DDP, VP-16, GBP and IFO, against two human hepatic carcinoma cell lines, SMMC-7721 and BEL-7402, was examined by MTT assay. The results showed that the cytotoxic effects of six chemotherapeutic agents with rhTNF-? against two cell lines were significant. Examined cytotoxicity of rhTNF-? combined with chemotherapeutic agents, we found that rhTNF-? singnificantly enhanced cytotoxic efficacy of ADM, DDP, VP-16. The study suggested that rhTNF-? with some chemotherapeutic agents had synergistic effects. This and other preclinical studies with rhTNF-? will form the basis of clinical treatments for hepatic carcinoma patient.
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Objective To investigate the inhibitory effect of SFAS on the growth of human hepatic carcinoma cell line SMMC-7721 in vitro and discuss its mechanism. Methods The inhibitory fraction of SMMC-7721 cells was measured by MTT assay, and both of the Annexin V and TUNEL assays were used to determine the apoptotic proportion. Results The value of the control group was (0.385?0.03), and that of the SFAS groups (250 ?g/ml, 500 ?g/ml, 1 mg/ml and 2 mg/ml) were (0.31?0.019), (0.199?0.022), (0.150?0.033) and (0.048?0.026) respectively, with significant difference statistically ( P