RESUMEN
Promoting hepatocyte growth factors are the small molecule polypeptide active substances which are extracted and purified from the fresh pig liver.Promoting hepatocyte growth factors are extensive in clinical application,used in treatment of acute hepatitis, chronic hepatitis, severe hepatitis, etc.This article introduces the clinical application of promoting hepatocyte growth factors on the treatment of viral hepatitis, in order to promote PHGF in reasonable clinical application.
RESUMEN
AIM: To observe the effects of sera containing Kang xian ling (a traditional Chinese medicine) on c-Met and its downstream MAPK signal molecules in HK-2 cells treated with transforming growth factor-β_1(TGF-β_1), so as to further explore the effective mechanism of anti-renal fibrosis of the traditional Chinese medicine. METHODS: Rat sera containing herbs were collected after rats were intragastrically administered with the herbs. HK-2 cells activated by TGF-β_1 were incubated with different heat-inactivated sera. The proliferation and expression of MAPK and the phosphorylation of MAPK in HK-2 cells were detected by Alarm blue and immunoblotting. RESULTS: No significant difference of cell proliferation between any two groups was observed. C-Met and the phosphorylation of its downstream molecules were also observed after incubated with different sera. CONCLUSION: The receptor c-Met of hepatocyte growth factor, and the phosphorylation of its downstream MAPK molecules can be regulated by the sera containing Kang xian ling.