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Objective:To repair knee joint defects in rabbits with rat adipose-derived stem cells induced by cartilage-derived morphogenetic protein growth factor 1 (CDMP1) ,so as to assess the feasibility of using heterogeneity cells as the seed cells for cartilage tissue engineering. Methods: The second generation ADSCs were seeded on scaffold, cultured for another two weeks in presence of CDMP1(50 μg/L),and identified by immunohistochemistory method. Bilateral rabbit knee joint defect model was established. The left side defect was embedded with ADSCs-scaffold composite (experimental group); the right side was embedded only with the scaffold(control group). Nine rabbits were killed in each group 8,16,24, and 48 weeks after embedding and the tissues were made into slices for safranine O and haematoxylin eosin staining. Results: In the experimental group the defects were filled with white semi-transparent tissues 8 weeks after embedding, with clear boundary to the surrounding cartilage; 16 weeks after embedding, the boundary of defect was further improved but still could be seen; 24 weeks after embedding, the repair outcomes were satisfactory, with the newly-generated chondrocytes having a nearly normal morphology (sphere shape,cartilage lacuna),and safranine O and haematoxylin eosin staining results were both positive; and 48 weeks after embedding, the boundary of the repair region could be clearly seen,and the repair effects were not as satisfactory as those of after 24 weeks. In the control group the boundary between the repairing area and the normal circumjacent area was visible at all 4 time points,with clear boundary and granulation tissues; the newly generated cells took a spindle shape and were negative for H-E and safranine O staining. Conclusion: The knee joint defects of rabbits can be satisfactorily repaired by using CDMP1-induced ADSCs seeded on spongy bone scaffold of cattle, which provides a theoretical basis for using heterogeneity cells as the seed cells for cartilage tissue engineering.
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Objective To investigate the anti-rejection effect of T cell vaccination combined with FK506 of heterogenic transplantation on rat nerve. Methods SD rats were vaccinated by the spleen cells of BABL/c mice; T cell vaccines were activated and vaccinated in the spleen cells of these SD rats. The SD rats of nerve transplantation recipient were vaccinated with T cells combined with FKS06. The mixture lymphocytes culture, microcytotoxicity assay, histological alteration of nerves, humid weights of tibial anterior muscle were observed to confirm the anti-rejection effect of T cell vaccination combined with FK506 treatment. Results The humid weights of tibial anterior muscle of the vaccinated rats combined with FKS06 treatment were statistically different from those in control group (P ＜ 0.05), and so as the index of rejection (e.g. lymphocytes transformation efficiency) (P ＜ 0.05). Conclusion T cell vaccination combined with FK506 treatment can inhibit the rejection of heterogenic transplantation on rat peripheral nerve.