RESUMEN
Three 2,3-diketoquinoxaline alkaloids were isolated from Heterosmilax yunnanensis Gagnep. Their structures were determined through 1D and 2D NMR, HR-ESI-MS, UV, and IR as 1-[5′-(3″-hydroxy-3″-methyl) glutaryl] ribityl-2,3-diketo-1,2,3,4-tetrahydro-6,7-dimethylquinoxaline (1), 1-[2′-(3″-hydroxy-3″-methyl) glutaryl]ribityl-2,3-diketo-1,2,3,4-tetrahydro-6,7-dimethylquinoxaline (2), and 1-ribityl-2,3-diketo-1,2,3,4-tetrahydro-6,7-dimethylquinoxaline (3). Compounds 1 and 2 are novel compounds, and 3 was isolated from H. yunnanensis for the first time. The hepatoprotective activity of these three compounds was evaluated, with compound 3 showing promising hepatoprotective activity.
RESUMEN
To minimize the predatory harvest of Heterosmilax yunnanensis and maintain the sustainable utilization of its resources, a study on the tending technology of wild H. yunnanensis was carried out. The results showed that the tuber tending model had a higher seed emergence rate, shorter growth period and easier control of male and female ratios than other tending models; by removing shrubs, topping, bending pruning, controlling insects and pests and other effective technical measures, the growth period of H. yunnanensis was shortened; the average annual net income of the tending area was 1 086 yuan/mu (1 mu≈666.67 m²), which was 86.9% higher than before. This study was conducive to increasing the yield and quality of H. yunnanensis in Karst landform area, and instructive for the tending of other wild traditional Chinese medicinal herbs in this area.
Asunto(s)
Femenino , Humanos , Masculino , SmilacaceaeRESUMEN
OBJECTIVE: To investigate the chemical differences between Smilacis Glabrae Rhizoma and its adulterants, and provide a basis for the identification and quality evaluation of Tufuling samples purchased from the markets. METHODS: The method of HPLC fingerprinting was used to analyze Smilacis Glabrae Rhizoma, Heterosmilacis Chinensis Rhizoma, Heterosmilacis Yunnanensis Rhizoma, and 102 Tufuling samples which were collected around China. The fingerprints were analyzed by the methods of similarity and principal component analysis (PCA). RESULTS: The analytical method of HPLC fingerprinting was established. Eleven, fifteen and eight common peaks were selected in the fingerprints of Smilacis Glabrae Rhizoma, Heterosmilacis Chinensis Rhizoma, and Heterosmilacis Yunnanensis Rhizoma, respectively. Only five common peaks were found in the fingerprints of the three species, which were No. 1, 2, 3, 13, and 14 peaks. A total of twelve peaks were characterized in the three fingerprints. Nine peaks were characterized in the fingerprint of Smilacis Glabrae Rhizoma, among which, four constituents were characterized for the first time. Six and two constituents were for the first time characterized in the fingerprints of Heterosmilacis Chinensis Rhizoma and Heterosmilacis Yunnanensis Rhizoma, respectively. The result of PCA analysis showed that the chemical differences between the three species were quite obvious and they could be distinguished from each other. The established method was used for the analysis of Tufuling samples purchased from the markets. Sixty-five samples were identified as Smilacis Glabrae Rhizoma, seventeen samples were identified as Heterosmilacis Yunnanensis Rhizoma, and twenty samples were identified as Heterosmilacis Chinensis Rhizoma. CONCLUSION: The established method is simple and reliable, and the development of fingerprint and its chemical pattern recognition provide the way and basis for identification of Smilacis Glabrae Rhizoma and its adulterants.
RESUMEN
Objective To study the chemical constituents of Heterosmilax yunnanensis.Methods The compound was isolated and repeatedly purified with chromatography and the structure was elucidated by physico-chemical properties and spectral analysis.Results A new compound was isolated and identified.Conclusion This compound is a novel compound with structure of 1,3,6,7-tetrahydroxy-xanthone-3-O-?-D-glucopyranoside,mamed heterosmioside A.