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OBJECTIVE:To study the effect and its possible mechanism of Xuezhiping capsule on blood lipid levels of high blood lipid model golden hamsters. METHODS:Golden hamsters were randomly divided into normal control group,model group, atorvastatin group (3 mg/kg),combination group (Xuezhiping capsule 1.3 g/kg+atorvastatin 1.5 mg/kg),Xuezhiping capsule low-dose,medium-dose,high-dose groups(Xuezhiping capsule 1.3,2.6,5.2 g/kg),10 in each group. Hamsters in normal control group received normal diet,the other groups were given high-fat diet to establish high blood lipid model. Then relevant drugs were intragastrically given 2 weeks later. Normal control group and model group were intragastrically given equal volume of distilled wa-ter,once a day,for 4 weeks. After last administration,blood lipid indexes(TG,TC,HDL-C,LDL-C,FFA),mRNA and protein expressions of lipid metabolism related genes (PPAR-α,CYP7A1,ACOX,SREBP-1c,ACC1) were detected. RESULTS:Com-pared with normal control group,serum contents of TC,TG,LDL-C,FFA in model group increased,HDL-C content decreased;PPAR-α,CYP7A1,ACOX mRNA and protein expressions were weakened,SREBP-1c,ACC1 mRNA and protein expressions were enhanced(P<0.05). Compared with model group,above-mentioned indexes were obviously improved in Xuezhiping capsule high-dose group,atorvastatin group and combination group (except for HDL-C in Xuezhiping capsule high-dose group)(P<0.05);TC,TG,FFA contents,and PPAR-α,CYP7A1,ACOX,ACC1 mRNA,and CYP7A1,SREBP-1c,ACC1 protein were obviously improved in Xuezhiping capsule medium-dose group(P<0.05);TC,TG contents,and PPAR-α mRNA,and CYP7A1, SREBP-1c protein were obviously improved in Xuezhiping capsule low-dose group (P<0.05). CONCLUSIONS:Xuezhiping cap-sule has a promising effect of lowering blood lipid,the mechanism may be related to activating PPAR-α and inhibiting SREBP-lc signaling pathway.
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Objective To explore the relationship between metabolic syndrome (MS) and obesity in obese children,and to explore the risk factors of MS.Methods The study included 56 obese children (26 boys and 30 girls) and 50 non-obese controls (25 boys and 25 girls).Anthropometry,fasting glucose,insulin concentrations,total cholesterol,high density lipoprotein (HDL),low density lipoprotein (LDL),triglycerides,systolic and diastolic blood pressure(BP) were measured in both of groups.Insulin resistance was determined by HOMA-IR.Finally subcutaneous and visceral fat thicknesses were measured by ultrasonographically.Results Obese children with metabolic syndrome had a high level of BMI,waist circumference,insulin levels,HOMA-IR,TG,blood pressure and visceral fat thickness than obese children without metabolic syndrome,but HDL-C was over than control group.The proportion of obese children with insulin ≥ 15 μU/ml,HOMA-IR ≥ 3.4 and TG ≥ 150mg/dl was significantly higher,while the proportion of HDL-C ＜ 40mg/dl was significantly lower than the control group.In logistic regression analysis showed that HOMA-IR ≥ 3.4,TG ≥ 150mg/dl and HDL-C ＜ 40mg/dl was strongly associated with the occurrence of MS and the risk was increased with the HOMA-IR score.The ORs of MS recurrence in obese children with HOMA-IR score between 3.4-4.9 was 3.8 compared with the reference group.That of HOMA-IR≥5 was 5.7,that of TG≥ 150mg/dl was 4.2,that of HDL-C ＜40mg/dl was 3.4 (P ＜0.05).Conclusion Children with HOMA-IR score between 3.4-4.9,HOMA-IR≥5,TG≥ 150mg/dl or HDL-C ＜ 40mg/dL have an significantly increased risk for metabolic syndrome.So for these obese children,we should advance screening of high-risk patients and intervene as early as possible to prevent the occurrence of metabolic syndrome and its complications.