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Objective To explore the diagnosis and treatment of the hypoxicisehemic encephalopathy of newborn in improving the healing rate and reducing the morbidity and mortality.Methods 56 cases from 2004 July to 2008 June were treated,all the clinical manifestations,diagnosis and treatment methods,causos and exmination were analyzed.Results Of 56 cases,clinical grading is mild 24cases;midrange 21cases;severe 11 cases;CT grading is mild 16 cases ;midrange 26 cases ;severe 14 cases;in this group,healing 34 cases,improving 16 cases,not healing 4 cases,death 2 cases,the total effective rate is 89.3%.Conclusion The causes of hypoxic-isehemic encephalopathy of newborn were complex and the clinical manifestations were veriform,the prognosis has intimate relations to the degree and commence of diagnosis and treatment.The morbidity,disable rate and mortality would be descended if proper diagnosis and prevention and cure means were given in the early period.
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Objective To investigate the effects of recombinant human erythropeietin(rh-EPO)on Bid mRNA and caspase-3 activity in the brain after hypoxic-ischemic brain damage(HIBD)in neonatal rats and the possible mechanism of the neuroprotective effect of rh-EPO.Methods Ninety 7 day old male SD rats weighing 12-18 g were randomly divided into 3 groups(n=30 each):group Ⅰ sham operation(S);group Ⅱ hypoxia-ischemia group(HI)and group Ⅲ rh-EPO.The animals were anesthetized with ether.Left common carotid artery was ligated with 4-0 silk and 3 days later the animals were placed in a 2 L airtisht vessel filled with 8%O2-92%N2 at 2-3 L/min for 2 h in group Ⅱ and Ⅲ.rh-EPO 3 000 IU/kg in 4 ml/kg normal saline(NS)was administered intraperitoneally after induction of hypoxia-ischemia(HI)in group Ⅲ while in group Ⅱ NS 4 ml/kg was given IP instead.Six animals in each group were killed at 6,12,24,48 and 72 h(T1-5)respectively after IP NS or rh-EPO.Their brains were removed for determination of Bid mRNA expression(by RT-PCR)and caspase-3 activity(by colorimetric method).Results The expression of Bid mRNA was up-regulated and caspase-3 activity was significantly increased in the brain at T1-5 in HIBD group(group Ⅱ)as compared with sham operation group.rh-EPO administration significantly reduced the increase in Bid mRNA expression and caspase-3 activity in the brain induced by hypoxia-ischemia.The expression of Bid mRNA was positively correlated with the caspase-3 activity.Conclusion rh-EPO has protective effects on the brain against hypoxia and ischemia by decreasing the expression of Bid mRNA and caspase-3 activity in the brain.