RESUMEN
@#<b>Objective</b> To investigate the differences in output results between difference analysis software, and to provide a reference for the improvement of quality control and the standard revision of SPECT equipment. <b>Methods</b> The image analysis software for quality control held by 14 radiological health technical service institutions was selected as the research subjects using the cluster sampling method. The results of the comparison were analyzed and evaluated according to the Z-score method with certified reference values. <b>Results</b> In the comparison items of “intrinsic uniformity” and “intrinsic spatial linearity”, the number of software with suspicious, deviated and incorrect results accounted for a relatively high proportion, while the results of other comparison items were satisfactory. <b>Conclusion</b> The applicability of SPECT image analysis software lacks effective supervision measures. Relevant departments should introduce feasible measures to standardize the software production and update process, accelerate the construction of radiological health information reporting platform, and promote the healthy development of the radiation health testing industry.
RESUMEN
Objective To investigate a new method using Image-Pro Plus (IPP) combined with Photoshop image analysis software to quantitatively measure the changes in microcirculation in hippocampus.Methods Twenty-two Japanese white rabbits that had received bilateral carotid artery ligation for 2 weeks without neurological dysfunction were divided into a subarachnoid hemorrhage (SAH) group and a control group, 11 rabbits in each group. The rabbit model of symptomatic cerebral vasospasm was established by the method of twice injecting blood into occipital cistern. On the 7th day after the first time of injecting blood, the rabbits were sacrificed, cerebral perfusion fixation was carried out, the hippocampus was harvested, and CD34 was determined by immunohistochemical determination. IPP 6.0 combined with Photoshop image analysis software was used to quantitatively measure the count of hippocampal microvessels density (MVD) and the field (for statistics)/microvascular capillary area ratio was calculated.Results CD34 could effectively identify microvascular endothelial cells, and using IPP 6.0 could automatically and accurately calculate MVD and field (for statistics)/micrevascular area ratio in hippocampus. Compared with the control group, in SAH group, the MVD and the area ratio in hippocampus were significantly reduced, the differences being statistically significant [MVD (count/area): 3.87±0.67 vs. 5.17±0.53, area ratio: (0.86±0.20)% vs. (1.40±0.17)%, bothP < 0.05].Conclusions CD34 can be used to identify microvessels, IPP 6.0 image analysis software co-Photoshop is a high efficient and accurate new method to measure the microvessels count and calculate the field/microvessel area ratio, not only it is easy to operate, but also the data can be automatically calculated and generated, reflecting precisely the changes in microcirculation in hippocampus after symptomatic cerebral vasospasm in rabbits.