The expression of autophagy and apoptosis in the epithelial tissue of oral submucous fibrosis / 中国现代医生

Objective To examine the expression of autophagy marker proteins LC3,P62 and apoptotic protein caspase-3 in oral submucous fibrosis(OSF)and investigate their relationship in epithelial mucosa.Methods To collect buccal mucosal specimens from 90 patients with OSF who underwent tissue pathological biopsy in the Department of Stomatology,Xiangya Hospital,Central South University from 2016 to 2019.There were divided into early stages of OSF group,moderately stages of OSF group,and advanced stages of OSF group.At the same time,30 healthy individuals without oral mucosal diseases were collected as control group.Compare the thickness of the epithelial cell layer using HE staining,and detect the expression of LC3,P62,and caspase-3 in the buccal mucosa epithelium of each group by immunohistochemistry.Results HE staining showed that compared to normal oral mucosa in control group,epithelial cell layer thickness of OSF patients was significantly reduce(P<0.05).Immunohistochemical staining showed that the expressions of LC3 and caspase-3 increased and P62 expression decreased significantly in the early stages of OSF group,moderately stages of OSF group,and advanced stages of OSF group(P<0.05).Their expressions were related to the mouth opening of patients with oral submucous fibrosis(P<0.05).In addition,the expression levels of LC3 and caspase-3 were positively correlated(r=0.320,P<0.05),the expression levels of P62 and caspase-3 were negatively correlated(r=-0.554 P<0.001),and the expression levels of LC3 and P62 were negatively correlated(r=-0.710,P<0.001).Conclusion Autophagy and apoptosis may be related to the occurrence and development of OSF,and the combined detection of LC3,P62,and caspase-3 is of great significance for the diagnosis of OSF.

Expression of galectin-1 in Chinese laryngeal squamous cell carcinoma patients and its clinical significance / 中华实验和临床病毒学杂志

Objective@#To observe the expression and clinical significance of galectin-1 in Chinese laryngeal squamous cell carcinoma patients.@*Methods@#Immunohistochemical technique was used to test the galecin-1 protein expression in the 50 laryngeal squamous cell carcinoma patients and Western blot method was used to test the galecin-1 protein expression in the 3 of the patients. SAS 9.4 software was used to analyze the relationship between the galectin-1 protein and the clinical degrees, pathological grades and lymph node metastasis in all the cases.@*Results@#In the 50 laryngeal squamous cell carcinoma patients, the positive rate of galectin-1 protein was 74% (37/50). The expression of galectin-1 protein was correlated with the severity of clinical staging, pathological differentiation and lymph node metastasis.@*Conclusions@#High expression of galectin protein related with the occurrence and development of head and neck squamous cell carcinomas.

The expression and clinical significance of Sox 11 in cervical carcinogenesis / 西安交通大学学报(医学版)

Objective To explore the expression level of Sox11 gene in cervical cancer cell lines and cervical pathology tissue .Methods The expression of Sox11 protein was detected in cervical cancer cell lines HeLa , CaSki ,SiHa and C-33A as well as NC ,LSIL ,HSIL and CC using immunohistochemistry and Western blot .We analyzed the relationship between Sox11 expression in cervical cancer and clinicopathologic parameters .Results The expression of Sox11 protein in normal cervical tissue ,low-grade cervical squamous intraepithelial neoplasia was significantly higher than that in the high-grade cervical squamous intraepithelial neoplasia and invasive cervical cancer tissue , and the expression was reduced with the progression of cervical lesions .The expression of Sox11 protein in the invasive cervical cancer was reduced with increased malignant degree .The expression of Sox11 protein in the invasive cervical cancer was significantly correlated with HPV infection ,but not associated with the age , clinical stage ,lymph node metastasis or muscular infiltration depth .Conclusion Sox11 expression is negatively correlated with cervical cancer development ,suggesting that as a tumor suppressor gene ,Sox11 may play a role in cervical cancer development and its absence or low expression is associated with the development of cervical cancer and is an early event of cervical carcinogenesis and may be a sign of malignant change of cervical tissue .

The Relationship of Expression of CD_(44)V_6, PCNA to Lymph Node Metastasis in Colorectal Carcinoma / 中国医师杂志

To explore the relationship of expression of metastasis associated protein-CD 44 V 6 and proliferating cell nuclear antigen(PCNA) to lymph node metastasis in colorectal carcinoma,randomly selected patients with colorectal carcinoma who underwent coloproctectomy plus D 2～D 3 lymph node dissection were studied.The 104 patients were divided into:A group (n=48) without lymph node metastasis and B group(n=56) with metastasis.The expressions of CD 44 V 6 and PCNA were assayed by immunohistochemical technique.The results indicated that the 56 cases(75%) of B group were positive for expressions of CD 44 V 6 and PCNA,there was obvious correlation between both the expressions and lymph node metastasis(P

CSF-1 and TGF-?1 Expression in periodontal tissue of third premolar germ during second primary molar orthodontic movement in dog / 实用口腔医学杂志

Objective: To investigate the expression of CSF-1 and TGF-?1 in periodontal tissue of third premolar germ during second primary molar orthodontic movement in dog. Methods: Orthodontic appliance with the force of 0.784 N for the mesial movement of mandible second primary molar was used in 4 dogs for 14 d.Then the samples including mandibular second and third molars and surrounding tissues were prepared for CSF-1 and TGF-?1 detection by immunohistochemical staining technique. Results: Strong CSF-1, TGF-?1 expression was found in the dental follicle and alveolar bone of mesial part of third premolar germ, and weak in the dental follicle and alveolar bone of distal part of third premolar germ in the treated side of the dogs. Conclusion: CSF-1 and TGF-?1 may play roles in the bone remodeling for orthodontic primary tooth movement.

Urinary Stone in Childen

Herpes simplex virus type 1 and 2 (HSV-1, HSV-2) are the ubiquitous human pathogens responsible for a variety of afflictions. HSV-2 is one of the viruses that were suspected of promoting carcinogenesis in the uterine cervix. Certainly, there is a need for the more sensitive and accurate laboratory techniques for HSV detection. We examined total 80 cases of smears including 17 Tzanck smears of skin and 63 cases of Papanicolaou smears from total 77 patients with clinical impression of herpetic infections, from September, 1985 through August, 1989. Immunohistochemical typings for HSV-1 and HSV-2 were performed together with routine cytologic findings and compared. The results are as follows ; 1) Patients were 9 males and 33 females, and age distribution was between 5 and 71 years 2) Subjective symptoms such as ulceration, vesicle, vaginal discharge, pruritus, and pain were complained in 36 patients and 38 cases were genital herpes. Recurrence was noted in 11 cases. 3) Positive results were obtained in 42 among 80 cases. 4) Both routine cytology and immunohistochemical staining were positive in 13 cases and in 24 cases only immunohistochemical staining were positive. 5 cases were positive only in routine cytologic smears. 5) The cases that immunocytochemical stain had been performed were 37 cases, which were all positive in type 2. Among the above 37 cases, type 1 also were positive in 5 cases. The results show that the immunoperoxidase technique is one of the rapid and reliable method to confirm the herpetic when suspected and that it is particularly useful when the Papanicolaou smear findings are equivocal.

Immunohistochemical Study of the Endotoxin Induced Uveitis in Rat

Using immunohistochemical technique, we observed time-sequentially to T and B lymphocyte in the ciliary body of the endotoxin induced uveitis in rat. The primary antibodies for immunohistochemical stain of the lymphocyte were IgM heavy chain, IgG heavy chain, T suppressor/cytotoxic (CD8) and T helper/macrophage (CD4) was performed in the frozen section. The intensity of immunohistochemical reaction for surface IgM positive cells increased from 12hours and reached most strong intensities at 24hours after endotoxin injection and then decreased rapidly, but the intensity for surface IgG positive cells increased from 48hours and sustained 96hours. There were no significant changes of intensity of immunohistochemical reaction for T cells except slightly increased T suppressor/cytotoxic cells (CD8).

Cellular components of proliferative vitreoretinal membranes

To understand the pathogenesis of proliferative vitreoretinal membrane formation which occurs in proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR), etc., accurate identification of the cellular components of the membrane is needed. This study was performed to identify cellular components of the membranes by means of immunohistochemical technique. 11 proliferative vitreoretinal membranes which were surgically obtained from 7 eyes with PVR and 4 eyes with PDR were stained with monoclonal antibodies against cytokeratin, glial fibrillary acidic protein (GFAP), or vimentin using immunoperoxidase technique (ABC method). In the PVR membranes, mean cell positivities for cytokeratin, GFAP and vimentin were 48%, 1% and 92%, respectively and in the PDR membranes, 0%, 5% and 93%, respectively. The above results suggest that retinal pigment epithelial cells and fibroblasts are major cellular components of PVR membranes, and that mesenchymal cells are major cellular components and glial cells are minor cellular components of PDR membranes.

Application of Immunohistochemical Technique in the Cytologic Diagnosis of herpes Simplex Virus Infection / 대한세포병리학회지

Herpes simplex virus type 1 and 2(HSV-1, HSV-2) are the ubiquitous human pathogens responsible for a variety of afflictions, HSV-2 is one of the viruses that were suspected of promoting carcinogenesis in the uterine cervix. Certainly, there is a need for the more sensitive and accurate laboratory techniques for HSV detection. We examined total 80 cases of smears including 17 Tzanck smears of skin and 63 cases of Papanicolaou smears from total 77 patients with clinical impression of herpetic infections, from September, 1985 through August, 1989. Immunohistochemical typings for HSV-1 and HSV-2 were performed together with routine cytologic findings and compared The results are as follows : 1) patients were 9 males and 33 females, and age distribution was between 5 and 71 years 2) Subjective symptoms such as ulceration, vesicle, vaginal discharge, pruritus, and pain were complained in 36 patients and 38 cases were genital herpes. Recurrence was noted in 11 cases. 3) Positive results were obtained in 42 among 80 cases. 4) Both routine cytology and imunohistochemical staining were positive in 13 cases and in 24 cases only immunohistochemical staining were positive. 5 cases were positive only in routine cytologic smears. 5) The cases that immunocytochemical stain had been performed were 37 cases, which were all positive in type 2, Among the above 37 cases, type 1 also were positive in 5 cases. The results show that the immunoperoxidase technique is one of the rapid and reliable method to confirm the herpetic infection when suspected and that it is particularly useful when the Papanicolaou smear findings are equivocal

DETECTION OF PLATELET MEMBRANE GLYCOPROTEIN WITH PAP-IMMUNOHISTOCHEMICAL TECHNIQUE / 中国法医学杂志

The platelets in the human mixed thrombus,hyaline thrombus antemortemand postmrtem skin incision wounds were detected by the PAP-immunohisto-chemical technique using monoclonal antibodies against the human platelet mem-brane glycoprotein(GPIb,GPIIIa).The positive reaction were observed inthe mixed thrombus,hyaline thrombus and in all the antrmortem skin incisionwounds,but not in the postmortem skin wounds.The significance of theapplication of this technique in the forensic medicine practice was discussed.