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Objective To analyze the levels of IL-35, IL-10 and TGF-β in women with or without pregnancy and to investigate the correlation between IL-35 and recurrent spontaneous abortion.MethodsLevels of IL-35, IL-10 and TGF-β in serum were analyzed by enzyme-linked immunosorbent assay (ELISA) in 120 gravidas with normal pregnancy, 40 gravidas with a history of recurrent spontaneous abortion, 40 healthy postpartum women and 40 healthy non-pregnant women of childbearing age.Single factor logistic regression analysis was used for correlation analysis.Results The level of serum IL-35 in normal pregnancies was significantly higher than that in non-pregnant women [333.6 (59.32, 1 391) pg/ml vs 123.9 (8.763, 471.7) pg/ml, P0.05].The level of serum IL-35 in gravidas with recurrent spontaneous abortion was significantly lower than that in healthy gravidas in their first trimester [220.4 (4.951, 702.0) pg/ml vs 386.5 (64.37, 1 355) pg/ml, P<0.05].Serum IL-35 was negatively correlated with the occurrence of recurrent spontaneous abortion (regression coefficient=-0.003, OR=0.997).Conclusion The level of serum IL-35 increases in healthy gravidas, but decreases in gravidas with recurrent spontaneous abortion.IL-35, rather than IL-10 or TGF-β, is recognized as an active player in maternal-fetal immune tolerance.
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Objective To explore the effect of chemotherapy on serum IL-6 expression in cancer patients with T2DM. Methods Eighty patients were divided into the malignant tumor group with T 2DM (n=40) and the malignant tumor group without T2DM (n=40).All the patients were given two cycles of chemotherapy .2 ml of fasting venous blood in the morn-ing before and after the day of chemotherapy was collected .Then the serum was separated .Enzyme-linked immunosorbent assay (ELISA)was used to measure the level changes of serum IL-6.20 cases of healthy people were selected for compari-son.Results The expression of serum IL-6 in groups with or without T2DM was significantly higher than in the normal control group before the first cycle of chemotherapy (P0.05).Conclusion The expression level of serum IL-6 declines after the first cycle chemotherapy in the malignant tumor groups with T2DM or without T2DM but rises in the malignant tumor group with T2DM after the second cycle chemotherapy .
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Immunosuppressive activities have been found in the culture supernatant of four leukemiacell lines and in the sera of four patients with leukemia. The inhibitors suppressed PHA-p in-duced lymphocytes proliferation and IL-2 production and responsiveness of T cells to IL-2, butno inhibitory activity was observed in the culture supernatant of 2BS cell line or in the sera ofnormal humam. The culture supernatant of tumor cell lines was not cytoxic. The result of PAGEshows: the molecular weight of TDSF from HL-60 human promyelocytic leukemia is about87KD. It is protein in chemical nature. The synthesis and sercretion of TDSF were partially in-hibited by drugs of anti-acute non-lymphocytic leukemia.