RESUMEN
Calculus Bovis is one precious traditional Chinese medicine used widely in clinical practice. Because of its high price and lack of resources, the substitutes of Calculus Bovis artificial Calculus Bovis, in vivo cultured Calculus Bovis and in vitro cultured Calculus Bovis also have been widely used in clinical practice. In order to understand the difference in chemical composition between Calculus Bovis and its substitutes, and determine whether all kinds of Calculus Bovis medicinal materials have special detection METHODS:, the chemical constituents and quality control METHODS: of Calculus Bovis and its substitutes were reviewed in this paper. Through the review of literature it was found that artificial Calculus Bovis mainly contain hyodeoxycholic acid and only a small amount of free bilirubin, and the chemical components of the other three kinds of Calculus Bovis medicinal materials are different. The chemical compositions of natural Calculus Bovis and in vitro cultured Calculus Bovis are similar, but the proportions of different types of bilirubin are different. At present, no characteristic ingredients of natural Calculus Bovis have been found to distinguish it from in vitro cultured Calculus Bovis and in vivo cultured Calculus Bovis.
RESUMEN
Calculus Bovis is the dry gall-stone of Bos taurus domesticus Gmelin, which is the most precious traditional Chinese medicine for its incomparable therapeutic effects. In vitro cultured Calculus Bovis,cultured Calculus Bovis and artificial Calculus Bovis are developed as the substitutes of Calculus Bovis. The Chinese Pharmacopoeia 2015 edition includes 89 Chinese patent drugs containing cow-bezoar and its substitutes. In this paper, the standards of Calculus Bovis and its substitutes in 89 Chinese patent drugs were reviewed to provide suggestions and reference for improving the relative standards. The qualities of Calculus Bovis and its substitutes of several Chinese patent drugs were summarized and analyzed based on the quality research of these drugs in recent years to offer suggestions for the revision of quality standards of relevant drugs and regulatory policy.
RESUMEN
OBJECTIVE: To develop a method for determination of different types of bilirubin in bezoar and explore the differences in their contents or ratios between natural and in vitro cultured Calculus Bovis to provide reference for identification of Chinese patent drugs containing bezoar. METHODS: Free bilirubin was determined directly by HPLC, while unconjugated and total bilirubin in bezoar were converted into free bilirubin respectively using different chelating agents. Converted bilirubin was eluted on a Diamonsil C18 column(4.6 mm×150 mm, 5 μm)using isocratic mobile phase composed of acetonitrile-1% acetic acid (95∶5) and detected at 450 nm. RESULTS: A small amount of free bilirubin was detected in Calculus Bovis but not found in in-vitro cultured Calculus Bovis. The content of total bilirubin in Calculus Bovis from different sources varied greatly while being similar in in vitrocultured samples. Bilirubin in covalent form occupied a great part in Calculus Bovis, and bilirubin in conjugated form hold a great proportionin in vitro cultured Calculus Bovis. CONCLUSION: The contents or ratios of different types of bilirubin can be used as the quality control parameters of naturaland in vitro cultured Calculus Bovis. The established method is easy to operate with high effectiveness in quality control, suggesting a new way of identifying bezoar.
RESUMEN
0.05).The results of electrocardiography and the laboratory ex-amination showed that neither ANWin cluding natural Calculus Bovis nor A NWincluding in -vitro cultured Calc ulus Bo-vis had obviously toxic and side effe cts in treating epidemic encephalitis B.Conclusion ANW including in -vitro cul-tured Calculus Bovis has an markedly effect in the treatment of epidemic e ncephalitis B.
RESUMEN
0.05).Conclusion In-vitro-cultured CB has good effects in the treatment of apoplexy.Neither in-vitro-cultrued CB nor natural CB for apoplexy has obvious adverse reaction.