Purification and functional assay of recombinant human interleukin 18 / 中国免疫学杂志

Objective:To purify recombinant human interleukin 18(rhIL 18) and perform activity assay of renatured rhIL 18 Methods:The rhIL 18 was purified by molecular sieve chromatography MTT assay and RT PCR method were used in activity assay of rhIL 18 Results:The purity of rhIL 18 was up to 96% The renatured rhIL 18 could enhance the cytotoxicity of NK cells and induce IFN ? gene expression in PBMC Conclusion:The rhIL 18 prepared exerts functional activity and can be applied for further studies