RESUMEN
Objective:To develop a UPLC method for determining four glycosides in microctis folium.Methods:The UPLC deter-mination was performed on an Agilent SB -C18 (3.0 mm ×50 mm, 1.8 μm) colume with 0.05% acetonitrile -phosphate solution as the mobile phase.The detection wavelength was set at 320 nm.The flow rate was 0.3 ml· min-1.The column temperature was 25℃.Results:Narcissoside, isovitexin, kaempferol-3-rutinoside and isorhamnetin-3-O-glucoside all showed good linearity with the re-covery of 98.89%,99.03%, 97.00%and 97.41%, and RSD of 0.41%, 0.84%,0.44%and 0.80%, respectively (n=9).Con-clusion:The method is convenient with good stability , repeatability and sample recovery rate , which provides basis for the quality eval-uation and control of microctis folium.
RESUMEN
Objective: To investigate the compouds from methylene dichloride and n-butyl alcohol parts of 70% ethanol extract of the fruits of Euscaphis fukienensis. Methods: The compounds were isolated and purified by Sephadex LH-20, silica gel column chromatographies, and recrystallization. Their structures were identified by physicochemical properties and spectroscopic methods. Results: Ten compounds were isolated and identified as 1, 2-benzenedicarboxylic acid (1), butanedioic acid (2), gallic acid (3), isorhamnetin-3-O-glucoside (4), 4-hydroxy-benzoic acid (5), 2-methyl-5, 7-dihydroxychrom one (6), muconic acid (7), quercetin-3-O- glycosidase (8), β-sitosterol (9), and daucostrol (10). Conclusion: Compounds 1-7 are isolated from the fruits of E. fukienensis for the first time.