Determination of carbohydrate chains in two kinds of microvascular endothelial cells by lectin cytochemistry / 解剖学报

Objective To determine the expression pattern of carbohydrate chains in two kinds of microvascular endothelial cells (MVECs). Methods Rat jejunal mucosal MVECs were thawed, and lung tissues were removed from specific pathogen free piglet of 3 days old to isolate and culture porcine pulmonary MVECs by collagerase digestion and differential attachment. By lectin cytochemistry, staining of 8 lectins including concanavalin A (Con A), phaseolus vulgaris erythroagglutinin (PHA-E), ricinus communis agglutinin I (RCA-I), lycopersicon esculentum lectin (LEL), sambucus nigra lectin (SNA), ulex europaeus agglutinin I (UEA-I), wheat germ agglutinin (WGA) and dolichos biflorus agglutinin (DBA) was detected in rat jejunal mucosal and porcine pulmonary MVECs. Results In rat jejunal mucosal MVECs, strong positve staining was present for Con A, WGA and LEL, medium one for PHA-E N SNA and RCA-I, weak one for DBA, and negative staining for UEA-I. In porcine pulmonary MVECs, strong positive staining was present for Con A and PHA-E, medium one in RCA-I, weak one for LEL and SNA, and negative staining for UEA-I, WGA and DBA. Conclusion The carbohydrate patterns in two kinds of MVECs display significant heterogeneity. Both rat jejunal mucosal and porcine pulmonary MVECs express mannose, galactose, 1, 3-N-acetylglucosamine and sialic acid at different levels. N-acetylglucosamine and N-acetylgalactosamine are detected in the former but not in the latter, and fucose do not in both MVECs.

The Effects Of Dietary Sodoium Nitrite Supplementation On Jejunal Mucosa And Possible Protective Effect Of Vitamin A In Albino Rats

Nitrate (NO3-) and nitrite (NO2-) are naturally-occurring oxidation products of nitrogen which widely used in thefood industry. The goal of this study was to illustrate the histopathological effects of sub-lethal dose of sodiumnitrite on jejunal mucosa and the possible protective effect of vitamin A. Thirty adult male Albino rats weredivided into 3 groups as follows: Group I (control) received distilled water, Group II (Nitrite-treated) whichreceived intra-gastric daily dose of 50 mg/kg NaNO2 and Group III (Nitrite & vit-A treated group) which receivedintra-gastric daily dose of 50 mg/kg NaNO2 +10,000 I.U/ rat vitamin A. Animals were sacrificed 8 weeks aftertreatment. Blood samples were collected and examined. Specimens from jejunal mucosa of rats were collectedfor the optical and electron microscope study. The blood samples showed significant reduction in all blood cellscounting and albumin level with elevated methemoglobin level in the nitrite treated group. Light microscopicexamination revealed that; Sodium nitrite cause jejunal mucosa damage represented by abnormal shape andlength of jejunal mucosa villi, necrosis with marked loss of covering epithelium and excessive cellular infiltrationin its core of lamina. PAS stained sections exhibited weak or negative PAS reaction of goblet cells of brush borderof villi and crypts. The entrocytes and goblet cells showed ultrastructural changes. Vitamin A administrationresulted in marked regression of the previously mentioned jejunal mucosal effects. Results of the current studyrevealed that diatry supplemtation of nitrite cause multible jejunal mucosal injury but the co-administration ofVitamin A greatly reduce the toxic effects of nitrite supplementation on jejunal mucosa

Sinergia entre aditivo vegetal e níveis crescentes de promotores de crescimento sobre o desempenho produtivo e morfometria intestinal de frangos de corte / Synergy between vegetal additive with increase levels of growth promoter on the performance and intestinal morphometry of broilers chickens

Objetivou-se avaliar o efeito da adição de níveis crescentes de antibióticos associados ao farelo integral de pimenta rosa (FIPR) sobre o desempenho produtivo e morfometria intestinal de frangos corte. Utilizaram-se 528 pintos Cobb, machos, distribuídos em um delineamento inteiramente casualizado, com seis tratamentos e quatro repetições de 22 animais cada, constituído pelos grupos T1 - Controle Negativo (CN) - Dieta basal sem FIPR e sem antibióticos; T2 - Controle Positivo (CP) - Dieta Basal com 1,2% FIPR; T3 - CP + 11ppm bacitracina zinco e 17ppm salinomicina; T4 - CP + 22ppm bacitracina zinco e 34ppm salinomicina; T5 - CP + 33ppm bacitracina zinco e 51ppm salinomicina; T6 - CP + 45ppm bacitracina zinco e 67ppm salinomicina. No período de 1-43 dias de idade, observou-se que a adição de FIPR com ou sem antibiótico reduziu (P≤0,05) o consumo e melhorou a conversão alimentar, comparado com CN. Verificou-se que os frangos alimentados com FIPR associado aos antibióticos, em valores acima de 22mg kg-1 de bacitracina de zinco e 34mg kg-1 de salinomicina, tiveram maiores alturas de vilosidades intestinais que o CN (P≤0,05). O FIPR pode ser utilizado em substituição aos antibióticos sem queda de desempenho animal e com a manutenção da viabilidade e do fator de produção.

The aim of this research was to evaluate the effects of the increase levels of antibiotics associated with the pink pepper meal (PPM) on animal performance and intestinal morphometry of broilers jejunum. A total of 528 day-old male chicks, Cobb 500, distributed in a randomized design of six treatments and four replicates of 22 birds each: T1 - Negative Control (NC) - basal diet without PPM or antibiotics, T2 - Positive Control (PC) - basal diet with 1,2% PP; T3 - PC + 11ppm zinc bacitracin and 17ppm salinomycin, T4 - PC + 22ppm zinc bacitracin and 34ppm salinomycin, T5 - PC + 33ppm zinc bacitracin and 51ppm salinomycin, T6 - PC + 45ppm zinc bacitracin and 67ppm salinomycin. In period of 1-43 days-old, it was observed that the use of PPM with or without antibiotcs reduced feed intake and improved feed:gain ratio compared with NC (P≤0,05). It was observed that the broilers fed diet with antibiotics values of up to 22mg kg-1 of zinc bacitracin and 34mg kg-1 of salinomycin associated with the PPM had the higher villus height compared with NC. The PPM can be used to replace antibiotic growth promoters without decrease on animal performance, viability and production factor.

Radioprotective Effects of Granulocyte-Colony Stimulating Factor in the Jejunal Mucosa of Mouse / 대한방사선종양학회지

PURPOSE: Granulocyte-colony stimulating factor (G-CSF) has been widely used to treat neutropenia caused by chemotherapy or radiotherapy. The efficacy of recombinant human hematopoietic growth factors in improving oral mucositis after chemotherapy or radiotherapy has been recently demonstrated in some clinical studies. This study was designed to determine whether G-CSF can modify the radiation injury of the intestinal mucosa in mice. MATERIALS AND METHODS: One hundred and five BALB/c mice weighing 20 grams were divided into nine subgroups including G-CSF alone group (I : 10 microgram/kg or II : 100 microgram/kg), radiation alone group (7.5 or 12 Gy on the whole body), combination group with G-CSF and radiation (G-CSF I or II plus 7.5 Gy, G-CSF I or II plus 12 Gy), and control group. Radiation was administered with a 6 MV linear accelerator (Mevatron Siemens) with a dose rate of 3 Gy/min on day 0. G-CSF was injected subcutaneously for 3 days, once a day, from day -2 to day 0. Each group was sacrificed on the day 1, day 3, and day 7. The mucosal changes of jejunum were evaluated microscopically by crypt count per circumference, villi length, and histologic damage grading. RESULTS: In both G-CSF I and II groups, crypt counts, villi length, and histologic damage scores were not significantly different from those of the control one (p>0.05). The 7.5 Gy and 12 Gy radiation alone groups showed significantly lower crypt counts and higher histologic damage scores compared with those of control one (p0,05). Most of the mice in 12 Gy radiation with or without G-CSF group showed intestinal death within 5 days. CONCLUSION: These results suggest that G-CSF may protect the jejunal mucosa from the acute radiation damage following within the tolerable ranges of whole body irradiation in mice.

Tropical sprue in a Filipino

The first case of mild Tropical Sprue in a Filipino is reported. The symptoms, as well as the microscopic changes in the jejunal and elial mucosa, were mild and non-specific. There was malabsorption of vitamin B12 which reverted to normal after a course of oral tetracycline and parenteral vitamin B12. The occurrence of tropical sprue in a Filipino and American visitors suggests a large reservoir of the disease exists in the Philippines

EFFECTS OF SOYBEAN PROTEIN ON JEJUNAL MUCOSA GROWTH IN GOAT LAMBS / 营养学报

Objective To determine the effect of soybean protein on growth of jejunal mucosa. Method Seventeen goat lambs at 7d of age were randomly allocated into control (casein group, n=9) and experimental group (soybean group, n=8). All lambs were fed milk mixture containing either casein or soybean protein (replaced 30% of casein). The lambs were slaughtered after 5 w and the tissues of jejunum were sampled to determine the villus morphology. The mucosa was collected for analysis of protein, nucleic acid content and the activities of alkaline phosphatase (ALP). Results The soybean administration induced villus atrophy. Protein content of mucosa tend to decrease both in proximal (127.2?4.6 vs 143.6?7.3 mg/g mucosa,P=0.07) and medial jejunum(111.2?6.3 vs 132.7?8.5 mg/g mucosa,P=0.06). In soybean group the activity of AP was lower(P