Kadsura coccinea: A rich source of structurally diverse and biologically important compounds / 中草药·英文版

Kadsura coccinea belongs to medicinally important genus Kadsura from the Schisandraceae family. It has been used in traditional Chinese medicine (TCM) for the treatment of rheumatoid arthritis and gastroenteric disorders. The initial phytochemical work focused on the identification of some structurally novel and diverse natural products, which turned the attention of many researchers towards this plant. Thus far, 202 compounds have been reported in this plant. Lignans and terpenoids were found as the main chemical constituents of this plant. Some of the triterpenoids and sesquiterpenoids with novel structures are of particular interest for natural product researchers. The isolated compounds of this plant have shown different bioactivities including anti-tumor, anti-HIV, anti-inflammatory, nitric oxide (NO) production inhibitory and other pharmacological effects. This review systematically summarizes all the phytochemical and pharmacological work done so far on K. coccinea, and can be used as a reference for future research on this plant.

Effects of Kadsura coccinea on Expression of Bcl-2, Bax and PCNA in Liver Tissue of Hepatic Fibrosis Rats / 医药导报

Objective To observe the effect of Kadsura coccinea on the expression of Bcl-2, Bax and proliferating cell nuclear antigen (PCNA) in liver tissue of experimental hepatic fibrosis rats, to further discuss the mechanism of anti-liver fibrosis. Methods Sixty SD rats were randomly divided into normal control group, model control group, colchicine group, low-dose Kadsura coccinea group (2.5 g·kg-1) and high-dose Kadsura coccinea group (5 g·kg-1) (n = 12) . All groups except the normal control group were treated with CCl4, rich fat and poor protein to establishexperimental hepatic fibrosis animal model. The second day after modeling, drug treatmentwas started, till the end of the sixth week. Pathological section of the rat' s liver was examined in order to observe its tissue under a optical microscope. Liver tissues were taken to examine the degree of liver fibrosis by HE and Masson staining, and the expression of Bcl-2, Bax and PCNA protein were detected by immunohistochemistry. Results Kadsura coccinea relieved the degree of necrosis of liver cells, liver fat' s degeneration and collagen fiber hyperplasia significantly. Compared with the model control group, expression of Bax in low-,high-dose Kadsura coccinea group and colchicine group were significantly decreased, and the expression of PCNA in hepatic fibrosis rats were enhanced(P<0.05 or P<0.01) . Conclusion Kadsura coccinea has a certain inhibitory effects on experimental hepatic fibrosis in rats, and its mechanism may be related to inhibiting the expression of Bax protein and promoting the expression of PCNA protein in liver tissues.

Effect of Kadsura coccinea Alcohol Extract on Immunologic Hepatic Fibrosis In Rats / 中国药学杂志

OBJECTIVE: To study the effects of Kadsura coccinea alcohol extract(KCAE) on rats with immunologic hepatic fibrosis and research the possible mechanisms in it. METHODS: Totally 60 SD male rats were randomly divided into 6 groups: a normal control group,a model group, a compound Biejia-ruangan tablets group(0.7 g•kg-1), KCAE high, middle and low dose groups(1.68, 0.84, 0.42 g•kg-1) at ten in each groups. Except for the normal control group,other groups were duplicated intraperitoneal injection of porcine serum twice a week at dose of 0.5 mL•time-1. The rats in treatment groups were intragastric administration respectively, meanwhile, the rats in normal control and model groups were treated with the same volume of distilled water, once a day for 15 weeks. The liver was weighed to calculate the liver index. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), total protein(TP), albumin(ALB) and total bilirubin(TB) were evaluated by the Mind-Ray automatic biochaemical analyzer. The expression level of procollagen III(PCIII), collagen type (-C), laminin(LN), hyaluronic acid(HA), transforming growth factor-β1(TGF-β1), interkeukin-10(IL-10), interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) in serum were detected by ELISA. The degrees of fibrosis were evaluated by HE and Masson straining, and the expression levels of TGF-β1 in liver tissue were assessed by Western blot. RESULTS: Compared with model group, the liver index of KCAE high-dose group was decreased significantly(P0.05). KCAE could decrease the level of PCIII, IV-C, LN, HA, TGF-β1, TNF-α and increase the level of IFN-γ in serum. KCAE could alleviate the hepatic fibrosis in rats(P<0.01) and inhibit the expression of TGF-β1 in the liver tissues significantly(P<0.01). CONCLUSION: KCAE has an anti-immunologic hepatic fibrosis effect in rats and the mechanisms possibly involve effectively regulating inflammatory cytokines, reducing extracellular matrix expression and inhibiting the expression of TGF-β1.

A high-performance liquid chromatography with circular dichroism detector for determination of stereochemistry of 6, 9-oxygen bridge dibenzocyclooctadiene lignans from kadsura coccinea / 中国天然药物

The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.

A lignin glycoside and a nortriterpenoid from Kadsura coccinea / 中国天然药物

AIM@#To study the chemical constituents of the roots and stem bark of Kadsura coccinea.@*METHOD@#Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS.@*RESULTS@#Two compounds were determined as (7'S,8'S,8R)-(8β,8'α)-dimethyl-4,4'-dihydroxy-5,3'-dimethoxy-5'-cyclolignan glucoside (1) and micrandiactone H (2), respectively.@*CONCLUSION@#Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide (NO) production in lipopolysaccharide-induced RAW264.7 macrophages.

Chemical constituents and their anti-oxidative activiities of Kadsura coccinea / 中草药

Objective: To study the chemical constituents and their anti-oxidative activities of Kadsura coccinea. Methods: The compounds were isolated and purified by column chromatography. The structures were determined by means of physicochemical properties and spectroscopic analyses. The anti-oxidative activity was studied by DPPH methods. Results: Fourteen compounds were separated and identified as micrandilactone C (1), 3, 4-dimethoxy phenyl-α-D-glucopyranoside (2), salidroside (3), schisanhenol (4), protocatechuic acid (5), ethyl shikimate (6), shikimic acid (7), 5-O-β-D-glucopyranosylgentisic acid (8), syringic acid (9), 1, 2-benzenedicarboxylic acid-dibuty ester (10), benzyl-O-β-D-glucopyranoside (11), 4-O-β-D-glucopyranosylvanillic acid (12), syringaldehyde (13), and methyl shikimate (14). Compounds 5, 6, and 14 had the anti-oxidative activities. Conclusion: Compounds 2, 4, 6, and 8-14 are firstly isolated from K. coccinea. Compounds 5, 6, and 14 have the certain prospects in the development of natural anti-oxidative agents.