RESUMEN
Objective: To investigate the phenolic compounds from Plumbago zeylanica and their anti-oxidant activities. Methods :The phenolic compounds and their analogues were isolated and purified by silica gel column chromatography, semi-preparative HPLC, and HPLC. Their structures were identified by spectroscopy data. The isolated compounds were tested for anti-oxidant activity by ABTS assay. Results: Twelve phenolic compounds were obtained and identified as (±)-5,5’-dimethoxyl ariciresionl 4’-O-β-D-glucopyranoside (1), tracheloside (2), quercetin-3-O-β-D-galactoside (3), quercetin-3-O-β-D-glucopyranoside (4), quercetin (5), quercetin 3-O-α-L-rhamnoside (6), kaempferol-3-O-α-L-rhamnoside (7), apigenin-6-C-β-D-glucopyranoside (8), luteolin 6-C-β- glucopyranoside (9), polybotrin (10), 3-O-(β-D-glucopyranosyl)-1-(3’,5’-dimethoxy-4’-hydroxyphenyl)-1-propanone (11), and (2E,4E,1’R,3’S,5’R,8’S)-dihydrophaseic acid-3’-O-β-D-glucopyranoside (12). The anti-oxidant activity experiment showed that compounds 3-9 displayed significant anti-oxidative activities. Conclusion: All of the twelve phenolic compounds are isolated from this plant for the first time. The isolated flavones 3-9 displayed significant anti-oxidative activities.
RESUMEN
Objective: To study the chemical constituents of Salacia cochinchinensis. Methods: The isolates were obtained by silica gel, Sephadex LH-20, MCI column chromatography, as well as prep-HPLC methods. Their structures were indentified on the basis of NMR, MS analyses. Results: Eight compounds were isolated from 90% ethanol extract of S. cochinchinensis. The structures were identified as 28-hydroxyl salaspermic acid (1), pachysandiol A (2), pristimeronol (3), 28-hydroxy-friedelan-3-one-29-oic acid (4), kaempferol-3-O-α-L-rhamnoside (5), 6,7-dimethoxy-4-hydroxyl-1-naphthoic acid (6), grasshopper ketone (7), 3,4,5-trimethoxyl benzoic acid methyl ester (8). Conclusion: Compound 1 is a new friedelane-type triterpenoid. Compounds 2-8 were isolated from the Salacia genus for the first time.
RESUMEN
OBJECTIVE: To study the chemical constituents of the ethnic drug Dicranopteris pedata. METHODS: The compounds were isolated and purified by comprehensive application of silica gel and Sephadex LH-20 chromatography, and their structures were identified on the basis of comprehensive analysis of their physical and chemical properties, NMR data and references. RESULTS: Fourteen compounds were isolated and elucidated as quercitrin(1), kaempferol 3-O-α-L-rhamnoside(2), rutin(3), hyperoside(4), kaempferol(5), quercetin(6),2-hydroxy-4-methoxy-2',3'-benzochalcone(7),stigmasterol(8), cycloastragenol(9), shikimic acid(10), protocatechuic acid(ll), gallic acid(12),β-sitosterol(13), and daucosterol(14). CONCLUSION: Compound 1-14 are for the first time isolated from D. pedata.
RESUMEN
Objective: To study the chemical constituents of Melilotus officinalis. Methods: The compounds were isolated and purified by silica gel column chromatography, gel chromatography, and ODS column chromatography. Their structures were elucidated by chemical and spectral analyses. Results: Twelve compounds were obtained from the 70% ethanol extract of M. officinalis and their structures were identified as β-sitosterol (1), coumarin (2), methyl 3-(4-hydroxyphenyl)propionate (3), kaempferol-3-O-α-L-rhamnoside (4), rutin (5), apigenin-7-O-β-D-lutinoside (6), dibutyl phthalate (7), robinin (8) clovin (9), kaempferol-3-O-rutinoside (10), isoquerecitrin (11), and medicarpin 3-O-β-glucopyranoside (12). Conclusion: Compounds 3, 4, 6, 7, 11, and 12 are isolated from plants from Melilotus Miller for the first time.