RESUMEN
Objective: Kangxian Pills, containing Angelicae Sinensis, Chuanxiong Rhizoma, radix paeoniae rubra, and 12 other kinds of Chinese materia medica, have the functions of softening and dispersing blood stasis. It has been used for liver injury and liver fibrosis. The current study was designed to evaluate the anti-hepatic injury activity and the mechanism of Kangxian Pills on a CCl4-induced animal model. Methods: To induce chronic liver injury, mice were treated with CCl4 twice a week for four weeks. Kangxian Pills (6 or 12 g/kg) and Compound Biejia Ruangan Tablet (0.901 g/kg) were ig given to mice once daily for four weeks after CCl4 was withdrawal. The anti-hepatic injury activities and mechanisms of Kangxian Pills were assessed by hepatic histology and by measuring the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), and total protein (TP) of serum as well as superoxide dismutase (SOD) and glycogen (Gn) of the liver. Results: Kangxian Pills significantly decreased the levels of liver index, ALT, and AST in mice liver injury models in treatment group. Moreover, Kangxian Pills and Compound Biejia Ruangan Tablet inhibited the CCl4-induced reduction of SOD and Gn levels in the liver. The histological study showed that Kangxian Pills could reduce cellular swelling and infiltration of inflammatory cells in liver injury. Conclusion: Kangxian Pills possess the potent abilities to alleviate chronic liver injury, suggesting that Kangxian Pills exert this effect by enhancing the anti-oxidant ability and metabolism of the liver.
RESUMEN
AIM: To establish the methods of quality control of Kangxian Pills (Redix Astragali,Radix Angelicale Sinensis,Rhizoma Chuanxiong,Radix Scutellariae,Fructus Schisandrae Chinensis,etc.). METHODS: Radix Angelicae sinensis;Rhizoma Chuanxiong;Radix Astragali;Radix Scutellariae were identified with TLC and the content of deoxyschizandrin and schisandrin B in the Kangxian Pills were determined by HPLC The separation was performed on Diamonsil~(TM) C_(18)(4.6 mm?250 mm,5 ?m)analytical column with a mobile phase consisted of methanol and water(78(∶)22) at the flow rate of 1.0 mL/min.The UV detection wavelength was set at 254 nm first and was altered to 220 nm after 17 min. RESULTS: The same color spots in the TLC graphs of sample existed at the corresponding position compared with the reference solution.The linear range of deoxyschizandrin was within 0.39-(1.95) ?g(r=0.999 9).The average recovery was 98.8%,RSD was 1.7%.The linear range of schisandrin B was within 0.09-0.45 ?g(r=0.999 9).The average recorery was 99.1%,RSD was 1.7%. CONCLUSION: The methods are simple and have good reproducibility.