Interference of non-intelligent factors in volleyball players' sports events / Interferência de fatores não inteligentes nos eventos esportivos dos jogadores de vôlei / Interferencia de factores no inteligentes en los eventos deportivos de los jugadores de voleibol

ABSTRACT Introduction Non-intelligent factors include learning habits, motivation, interest, emotion, attitude, and student characteristics. Many sports practices have demonstrated that creating excellent athletic performance and winning intense competition depends on various factors. Among them, physical quality is the physiological and material basis to ensure the quality of exercise. Movement technique is the essential condition. However, non-Intelligent factors are the internal motivators for both to function. Objective Analyze the non-Intelligent factors that affect the performance of volleyball players. Methods Several volleyball players were selected as research objects. The non-Intelligent factors that affect volleyball performance are analyzed by questionnaire survey and experimental method. Finally, this paper uses mathematical statistics to analyze the experimental data. Results Volleyball players are easily disturbed by external factors. These non-Intelligent factors can easily lead to large fluctuations in the athlete's psychology. These reasons will affect the stability of volleyball players' serving skills. Conclusion The non-Intelligent factors that affect the performance of volleyball players are the proficiency of serving technique, the degree of psychological relaxation, and the ability of emotional control. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução Fatores não-inteligentes incluem hábitos de aprendizagem, motivação, interesse, emoção, atitude e características estudantis. Muitas práticas esportivas têm demonstrado que criar um excelente desempenho atlético e vencer competições intensas depende de vários fatores. Entre eles, a qualidade física é a base fisiológica e material para garantir a excelência do exercício. A técnica do movimento é a condição essencial. Entretanto, fatores não inteligentes são os motivadores internos para que ambos funcionem. Objetivo Analisar os fatores não-inteligentes que afetam o desempenho dos jogadores de vôlei. Métodos Vários jogadores de voleibol foram selecionados como objetos de pesquisa. Os fatores não-inteligentes que afetam o desempenho do voleibol são analisados por questionário e método experimental. Finalmente, este artigo utiliza estatísticas matemáticas para analisar os dados experimentais. Resultados Os jogadores de vôlei são facilmente perturbados por fatores externos. Estes fatores não-inteligentes podem facilmente levar a grandes flutuações na psicologia do atleta. Estas razões afetarão a estabilidade das habilidades de serviço dos jogadores de vôlei. Conclusão Os fatores não-inteligentes que afetam o desempenho dos jogadores de vôlei são a proficiência da técnica de saque, o grau de relaxamento psicológico e a capacidade de controle emocional. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Los factores no inteligentes incluyen los hábitos de aprendizaje, la motivación, el interés, la emoción, la actitud y las características del alumno. Muchas prácticas deportivas han demostrado que la creación de un excelente rendimiento deportivo y la obtención de una intensa competición dependen de varios factores. Entre ellos, la calidad física es la base fisiológica y material para garantizar la calidad del ejercicio. La técnica de movimiento es la condición esencial. Sin embargo, los factores no inteligentes son los motivadores internos para que ambos funcionen. Objetivo Analizar los factores no inteligentes que afectan al rendimiento de los jugadores de voleibol. Métodos Se seleccionaron varios jugadores de voleibol como objeto de investigación. Los factores no inteligentes que afectan al rendimiento de los jugadores de voleibol se analizan mediante un cuestionario y un método experimental. Por último, este trabajo utiliza la estadística matemática para analizar los datos experimentales. Resultados Los jugadores de voleibol son fácilmente perturbados por factores externos. Estos factores no inteligentes pueden provocar fácilmente grandes fluctuaciones en la psicología del deportista. Estas razones afectarán a la estabilidad de las habilidades de saque de los jugadores de voleibol. Conclusión Los factores no inteligentes que afectan al rendimiento de los jugadores de voleibol son el dominio de la técnica de saque, el grado de relajación psicológica y la capacidad de control emocional. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Preparation of national reference panel of HBV for nucleic acid testing donor screening / 中国生物制品学杂志

@#Objective To prepare the national reference panel of hepatitis B virus(HBV) for nucleic acid testing(NAT)donor screening.Methods A number of plasma samples from donors positive for HBV antibody and patients with HBV infection collected from blood centers,plasma stations and biological products companies in Shanghai,Gansu,Henan,Hunan,Hubei and other regions were tested for HBV DNA viral load agent,and negative and positive reference candidates were screened;The HBV DNA national standard was diluted to 10~3 IU/ml with human negative plasma,as a candidate for limit of detection(LOD).National negative and positive reference candidates of HBV for NAT donor screening and LOD reference to be calibrated were distributed to 8 enterprise laboratories for joint detection of HBVHCVHIV NAT donor screening.The homogeneity and stability of the national reference panel were investigated.Results A total of 8 negative samples with HBV viral load of 0 were screened as negative references and 9 positive samples with viral load of 10~3～10~4 IU/mL were used as positive references;One LOD reference was calibrated with WHO HBV DNA standard,and the virus content was 1.0 × 10~3 IU/ml.The national reference panel showed good stability and the homogeneity inspection met the requirements.Conclusion The national reference panel of HBV DNA for NAT donor screening was prepared,which provided a basis for the quality control and standardization of HBV DNA reagents for donor screening.

Preliminary study on cerebrospinal fluid proteomics of Erxian Decoction against neurogenesis impairment in late-onset depression / 中国中药杂志

This study aims to elucidate the underlying mechanism of Erxian Decoction(EXD) against neurogenesis impairment in late-onset depression(LOD) rats based on cerebrospinal fluid(CSF) proteomics. A total of 66 20-21-month-old male Wistar rats were randomized into naturally aged(AGED) group, LOD group, and EXD group. All rats received chronic unpredictable mild stress(CUMS) for 6 weeks for LOD modeling except for the AGED group. During the modeling, EXD group was given EXD(ig, twice a day at 4 g·kg~(-1)) and other groups received equivalent amount of normal saline(ig). After modeling, a series of behavioral tests, such as sucrose preference test(SPT), open-field test(OFT), forced swimming test(FST), and Morris water maze test(MWMT) were performed. Immunofluorescence method was used to detect the number of Ki-67/Nesti-positive cells and BrdU/DCX-positive cells in the hippocampal DG area of each group. High-concentration corticosterone(CORT) was combined with D-galactose(D-gal) to simulate the changes of LOD-related stress and aging and the proliferation and differentiation of primary neural stem cells of hippocampus in each group were observed. Data independent acquisition(DIA)-mass spectrometry(MS) was used to analyze the differential proteins in CSF among groups and bioinformatics analysis was performed to explore the biological functions of the proteins. Behavioral tests showed that sucrose consumption in SPT, total traveling distance in OFT, and times of crossing the platform in MWMT were all reduced(P<0.01) and the immobility time in FST was prolonged(P<0.01) in the LOD group compared with those in the AGED group, suggesting that LOD rats had developed depression symptoms such as anhedonia, decreased locomotor activity ability, and cognitive dysfunction. Behavioral abnormalities were alleviated(P<0.01, P<0.05) in the EXD group as compared with those in the LOD group. Immunofluorescence results demonstrated that Ki-67/Nesti-positive cells and BrdU/DCX-positive cells in the hippocampal DG area were fewer(P<0.05) in LOD group than in the AGED group, and the positive cells in the EXD group were more(P<0.05) than those in the LOD group. In vitro experiment showed that the proliferation and differentiation of primary hippocampal neural stem cells under the CORT+D-gal treatment were reduced(P<0.01). The proliferation rate of neural stem cells decreased(P<0.05) in CORT+D-gal+LOD-CSF group but increased(P<0.01) in CORT+D-gal+EXD-CSF group compared with that in the CORT+D-gal group. A total of 2 620 proteins were identified from rat CSF, with 135 differential proteins between the LOD group and AGED group and 176 between EXD group and LOD group. GDF11, NrCAM, NTRK2, and GhR were related to neurogenesis and 39 differential proteins were regulated by both LOD and EXD. EXD demonstrated obvious anti-LOD effect, as it improved the locomotor activity ability and cognitive function of LOD rats and protected the proliferation and differentiation of hippocampal neural stem cells. EXD exerts anti-LOD effect by regulating the proteins related to neurogenesis in CSF, such as GDF11, NrCAM, NTRK2, and GhR and maintaining hippocampal neurogenesis.

Safety issues and new rapid detection methods in traditional Chinese medicinal materials

The safety of traditional Chinese medicine (TCM) is a major strategic issue that involves human health. With the continuous improvement in disease prevention and treatment, the export of TCM and its related products has increased dramatically in China. However, the frequent safety issues of Chinese medicine have become the 'bottleneck' impeding the modernization of TCM. It was proved that mycotoxins seriously affect TCM safety; the pesticide residues of TCM are a key problem in TCM international trade; adulterants have also been detected, which is related to market circulation. These three factors have greatly affected TCM safety. In this study, fast, highly effective, economically-feasible and accurate detection methods concerning TCM safety issues were reviewed, especially on the authenticity, mycotoxins and pesticide residues of medicinal materials.

Establishment and Evaluation of Blank Limit,Detection Limint and Quantitation Limit of Fecal Occult Blood Tests with Colloidal Gold Method Based on the Document of EP-1 7A2 / 现代检验医学杂志

Objective To explore colloidal gold method used to detect fecal occult blood tests(FOB)detection capability and establish the laboratory standard operation of detecting FOB limit of blank(LOB),limit of detection (LOD)and quantifica-tion limit (LOQ)according to the CLSI document《Evaluation of Detection Capability for Clinical Laboratory Measurement Procedures;Approved Guideline-Second Edition》(EP17-A2),in order to reduce the false negative rate of the weakly positive samples,and to provide a way of quantitative detection for qualitative detection of colloidal gold method.Methods Detected series of solution of hemoglobin made of dissolved fresh whole blood with the ELISA kit of human free hemoglobin,and es-tablished the standard curve of detection of FOB with colloidal gold method.Detected the blank samples and a series of low concentration samples with the colloidal gold test strip of FOB and measured the color bands by the Nato Checker710.The quantitative results obtained were statistically analysised by SPSS 1 9.0 and calculated blank limit,detection limit and quanti-fication limit.Results The LOB,LOD and LOD were 99.01,340.48 and 354.9 ng/ml according to the methods in CLSI EP1 7-A2 ducument.Conclusion The detection limits established by CLSI EP1 7-A2 document was more scientific in j udge-ment positive or negative to FOB than which used naked eye and can meet the clinical laboratory and clinical doctor require-ment better.Clinical laboratories should be strictly in detection limits of reagents in order to ensure their effectiveness,and should be generaly to other tests based on colloidal gold method.

Linkage analysis of three families with arrythmogenic right ventricular cardiomyopathy in India.

BACKGROUND: Arrythmogenic Right Ventricular Cardiomyopathy (ARVC) is a primary myocardial disorder morphologically characterized by subtle to severe replacement of the right ventricular myocardium by fatty and fibrous tissue. ARVC is known to be highly prevalent in European population with recent reports implicating it to be a major cause of sudden death in young individuals even from American and Asian population. AIM: To implicate or exclude TMEM43 (ARVC-5), DSP(ARVC-8) genes and the yet to be identified gene at ARVC-6 locus in the pathogenesis in three families affected with ARVC from India. MATERIALS AND METHODS: Three families comprising of 42 affected/unaffected members were included in the study. Three microsatellite markers, D3S3613 (ARVC5) D10S1664 (ARVC6), D6S309 (ARVC8) were genotyped by PCR-based native PAGE. Two-point Linkage analysis was performed using LINKAGE program version 5.2 RESULTS AND DISCUSSION: LOD scores from linkage analysis for the microsatellite marker D10S1664 (ARVC-6) in families KS and REV have shown positive value hinting the involvement of this locus in the etiology of ARVC, while linkage analysis in the SB family ruled out involvement of DSP, TMEM43 and ARVC-6, as negative LOD scores were obtained with all three loci. Therefore, linkage analysis carried out in the present study indicates that ARVC-6 (cumulative LOD score is equal to plus 1.203376 at θ is equal to 0.05) could be the locus harboring the mutated gene in two out of three families.