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OBJECTIVE：To investigate the composition-activity relationship of the antioxidant active component in essential oil of Lavandula angustifolia from Xinjiang. METHODS ：Antioxidant activity of essential oil of L. angustifolia was evaluated with 1，1-diphenyl-2-picrylhydrazyl（DPPH）free radicals scavenging method. GC-MS combined with retention index were adopted to qualitatively analyze the compounds. The relationship of chemical components with antioxidant activity of essential oil of L. angustifolia was analyzed by principal component analysis （PCA） and partial least squares analyses （PLS）. The variable importance in projection （VIP＞1）was used to screen the components which had great contribution to the elimination of DPPH free radical. RESULTS ：The IC 50 values of essential oil in 9 batches of L. angustifolia from 3 different varieties was 4.82-9.88 mg/mL，IC50 of France blue was 6.66-8.58 mg/mL，IC50 of H- 701 and Xinxun- 4 were 4.82-7.73 mg/mL and 9.55-9.88 mg/mL，respectively. A total of 40 chemical components were identified of essentil oil in 9 batches of L. angustifolia . Results of PCA analysis showed that accumulative variance contribution rate of former 2 main components was 81.8％；9 batches of samples were divided into 3 regions， and different varieties were classified into one category ；the characteristic variable components of the essential oil of French blue samples were lavandulyl acetate corresponding to peak 32 and trans- β-ocimene corresponding to peak 9；the characteristic variable components of the essential oil of H- 701 samples were linalool corresponding to peak 16，linalyl acetate corresponding to peak 30 and terpinen- 4-ol corresponding to peak 21；the characteristic variable components of the essential oil of Xinxun- 4 samples were eucalyptol corresponding to peak 8，camphor corresponding to peak 18 and 2-borneol corresponding to peak 20. The compound with the greatest antioxidant activity was linalool corresponding to peak 16 （VIP＝2.940 0），followed by terpenein- 4-ol corresponding to peak 21（VIP＝2.863 1），caryophyllene corresponding to peak 35（VIP＝2.570 8）and eucalyptol corresponding to peak 8（VIP＝2.115 8）. CONCLUSIONS ：The essential oil of L. angustifolia has certain antioxidant activity ，and H- 701 sample has higher antioxidant activity. Linalool corresponding to peak 16 is the most important component of essential oil of L. angustifolia in eliminating DPPH free radical.
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Lavender is well-known for its essential oils, which are in high demand and have a very important economicinterest, particularly for the pharmaceutical and cosmetic industries. This work had been done to study seedgermination and in vitro culture of Lavandula angustifolia. The seeds of L. angustifolia had undergonea vernalization followed by two treatments (physical and chemical), in order to increase the percentage ofgermination. Seeds were introduced into tubes containing the culture medium Murashige et Skoog (MS).Untreated seeds were germinated in the same medium supplemented with gibberellic acid at differentconcentrations. The kinetics, the speed, and the final germination rate were retained in order to evaluate theresponse of seeds. The micropropagation of L. angustifolia was performed on MS medium supplemented withdifferent concentrations of 6-Benzylaminopurine (Treatment I) and 6-Benzylaminopurine in combination withNaphthalene Acetic Acid (Treatment II). Multiplication rate, shoots number, nodes number, leaves number, andstem length were determined. Lavandula angustifolia seeds revealed very low germination percentages for allthe treatments used. However, those treated with sand (physical treatment) showed the highest percentage ofgermination (22%) followed by sulfuric acid treatment with a percentage of 14%. Results showed that the 11.11μM concentration of 6-Benzylaminopurine favored the maximum reactivity of the explants. The combinationbetween 6-Benzylaminopurine and Naphthalene Acetic Acid showed that MS supplemented with 8.88 μM6-Benzylaminopurine and 2.68 μM Naphthalene Acetic Acid was the most effective in the development ofvitroplants.
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Objective: To investigate the lignans compounds constituents of Lavandula angustifolia. Methods: The chemical constituents were isolated and purified by TLC, silica gel, MCI-gel, and RP-HPLC, and their structures were identified by analysis of spectroscopic evidences and physicochemical properties. Results: A total of 11 constituents were isolated from L. angustifolia and elucidated as pinoresinol (1), syringaresinol (2), fraxiresinol-4'-O-β-D-glucopyranoside (3), syringaresinol-4'-O-β-D-glucopyranoside (4), 8-hydroxypinoresinol-4-O-β-D-glucopyranoside (5), rel-(2α,3β)-7-O-methylcedrusin (6), lariciresinol-4'-O-β-D-glucoside (7), (2S,3R)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxybenzofuran-5-(trans) propen-1-ol-3-O-β-glucoside (8), (7S,8R)-dihydrodehydrodiconiferyl alcohol-9-β-D-glucopyranoside (9), (7R,8R)-7,8-dihydro-9'-hydroxyl-3'-methoxyl-8- hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1'-benzofuranpropanol-9'-O-β-D-glucopyranoside (10), and (E)-3-((2S,3S)-2-(4- hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxy-2,3-dihydrobenzofuran-5-yl) allyl-2-hydroxyacetate (11). Conclusion: The 11 compounds are isolated from this plant for first time.
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Candida sp. especially C. albicans is the main cause of candidiasis in women in reproductive ages. The prevalence of drug resistant C. albicans along with adverse effects of current treatments have encouraged the scientists to research on medicinal plant's essential oils. The aim of this study was to evaluate the potential effects of Ziziphora tenuior, Lavandula angustifolia, Cuminum cyminum essential oils against clinical isolates of C. albicans, which were isolated from women with vulvovaginal candidiasis. The anti-candidal effects of these commercial essential oils were screened against these clinical isolates (n=80) by disc diffusion and micro-broth dilution assays. The means of inhibition zone diameters, MIC and MFCs were reported for each essential oil. Also, the capability of fungal strain for biofilm formation in comparison with C. albicans ATCC 10231 were determined. The anti-biofilm effects of essential oils against 11 biofilm producing strains of C. albicans were determined. The results showed C. cyminum and Z. tenuior essential oils had the best anti-candidal effects, while the best biofilm killing effects was for L. angustifolia essential oil. C. cyminum and Z. tenuior essential oils can be formulated for more evaluations in preclinical and clinical studies.
Candida sp., especialmente C. albicans, es la principal causa de candidiasis en mujeres en edad reproductiva. La aparición de resistencia a los antifungicos de C. albicans, junto con el riesgo de efectos adversos de los tratamientos actuales, ha llevado a los científicos a buscar alternativas en los aceites esenciales derivados de plantas. Los objetivos del estudio fueron evaluar los efectos potenciales de los aceites esenciales de Ziziphora tenuior, Lavandula angustifolia, Cuminum cyminum, contra aislados clínicos de C. albicans, obtenidos de mujeres con candidiasis vulvovaginal. Los efectos anti-Candida de estos aceites esenciales comerciales fueron probados contra estos aislados clínicos (n=80) por difusión en disco y ensayos de microdilución. Se obtuvo el promedio de diámetro de inhibición, MIC y MFCs para cada aceite esencial. También se comparó la capacidad de formación de biopelículas de 11 cepas de C. albicans de cada aislado frente a la cepa de referencia C. albicans ATCC 10231 y la capacidad de cada aceite esencial para evitar la formación de biopelículas. Los resultados muestran que los aceites esenciales de C. cyminum y Z. tenuior tuvieron la mejor actividad anti-Candida, mientras que los mejores efectos para destruir biopelículas se obtuvieron con los aceites esenciales de L. angustifolia. Los aceites esenciales de C. cyminum y Z. tenuior ameritan tener mayores evaluaciones preclínicas y clínicas.
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Humanos , Femenino , Adulto , Candida albicans , Candidiasis Vulvovaginal , Aceites Volátiles , Biopelículas , Cuminum , LavandulaRESUMEN
ABSTRACT: Lavender is an aromatic ornamental plant that is used widely in the perfume, pharmaceutical, cosmetical and food industries. That is why it is important to study ways to promote a production of lavender raw material. For this, four experiments were carried out in order to study the germination and initial in vitro development of lavender. In the first part the efficiency of the use of hydrogen peroxide in the pre-treatment of seeds inoculated in culture media with different concentrations of gibberellic acid was evaluated. Besides that, the influence of the use of Growlux lamps and white fluorescent lamps on plantlets cultivated in MS and LS media at salts concentrations of 50 and 100% was evaluated. Finally, the effect of the gibberellic acid, putrescine, spermine and spermidine in different concentrations was evaluated. Better results were estimated when hydrogen peroxide pretreatment was applied to the seeds with subsequent inoculation in a medium containing 2,5 mg L-1 of gibberellic acid. The use of Growlux lamps did not influence the characteristics observed when compared to the use of white fluorescent lamps, however it was concluded that the species develops more in a LS medium. Concerning the use of polyamines was found that germination, aerial part and number of leaves of the seedlings were generally favored at the concentration of 0,5 mg L-1 except when spermine was applied, which resulted in a lower number of leaves under this condition.
RESUMO: Lavanda é uma planta ornamental aromática que é amplamente utilizada nas indústrias de perfumaria, farmacêutica, cosmética e alimentícia e por isso é importante estudar maneiras de promover a produção dessa matéria-prima. Para tanto, foram realizados quatro experimentos com o objetivo de estudar a germinação e o desenvolvimento inicial in vitro da lavanda. No primeiro experimento testou-se a eficiência do uso de peróxido de hidrogênio no pré-tratamento de sementes inoculadas em meios de cultura com diferentes concentrações de ácido giberélico. Além disso, foi avaliado a influência do uso de lâmpadas Growlux e de lâmpadas fluorescentes brancas em plântulas cultivadas nos meios MS e LS em concentrações de sais de 50 e 100%. Por fim, também estudou-se o efeito dos reguladores ácido giberélico, putrescina, espermina e espermidina em diferentes concentrações. Melhores resultados foram encontrados quando o pré-tratamento com peróxido de hidrogênio foi aplicado às sementes com posterior inoculação em meio contendo 2,5 mg L-1 de ácido giberélico. O uso de lâmpadas Growlux não influenciou as características avaliadas quando comparado ao uso de lâmpadas fluorescentes brancas, entretanto observou-se que as espécies se desenvolvem melhor em meio LS. Com relação ao uso de poliaminas, verificou-se que a germinação, parte aérea e número de folhas das plântulas foram favorecidas na concentração de 0,5 mg L-1, exceto quando se aplicou espermina, que resultou em menor número de folhas nessa condição.
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OBJECTIVE:To establish the quality standard for Compound Lavandula angustifolia ointment. METHODS:TLC was used for the qualitative identification of ethanol extract from Scutellaria baicalensis and volatile oil of L. angustifolia. GC method was used for qualitative identification of dementholized peppermint oil. GC method was used to determine the content of menthol. The determination was performed on Agilent DB-WAX capillary column,with temperature programming. The injector temperature was 250℃,and the temperature of detector was 250℃.The injection volume was 1 μL and the split ratio was 5:1 by split sampling. RESULTS:TLC spots of ethanol extract of S. baicalensis and volatile oil of L. angustifolia were clear and well-repeated without interference from negative control. The chromatographic peaks in TLC of test samples of dementholized peppermint oil had same retention time as that of substance control.The linear range of menthol injection amount was 0.113 4-1.133 5μg (r=0.999 4). RSDs of precision,intra-day precision,stability and reproducibility tests were not higher than 2.0%. The recoveries were 95.40%-99.82%(RSD=1.61%,n=6). CONCLUSIONS:Established quality standard can be used for the quality control of Compound L.angustifolia ointment.
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Lavandula angustifolia (Family Labiates) is a medicinal herb found in Mediterranean area. It is a well known herb in ayurvedic system of medicines and has traditionally been used to treat disorder of liver, fever and several conditions including infertility, infection and anxiety. There are few reports on tissue culture of Lavandula angustifolia that too mainly on micropropagation. Present study explored an in vitro micropropagation of Lavandula angustifolia. In vitro callus formation was established by using nodal segments on Murashique and Skoog, (1962) medium (MS) supplemented with IAA at 0.1mg/l, l BAP at 0.002mg/l and 2-4D at 0.2mg/l, significantly recorded complete callus formation after 6 weeks of incubation at 25±1ºC. The callus was allowed for organogenesis and then shoot multiplication was carried out at 4 concentrations of BAP (0.5, 1, 2 and 1mg/L) and IAA (0.5, 0.5, 0.5 and 1mg/L) on MS medium. The shoot regeneration medium for shoot multiplication and proliferation with higher number of shoots was recorded at 0.5mg/l of IAA and 2.0mg/l BAP. However, the growth was very steady and originating from the base. MS medium without any growth regulators tabulated the tallest shoot length of 35 mm, but the shoots were clustered not properly differentiated.
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Objective To establish the fingerprints of 20 batches of Lavandula Angustifolia by HPLC. Methods The determination was performed on a Phenomenex ODS-A column (250 mm× 4.6 mm, 5 μm). The mobile phase was in gradient elute mode with a mixture consisting of acetonitrile and 0.036 mol/L phosphate acid solution. The flow rate was 1.0 mL/min. The temperature was 30 °C. The determine wavelength was 350 nm. The fingerprints of 20 batches of Lavandula Angustifolia were compared and classified by similarity evaluation, cluster analysis, and principal composition analysis. Results Totally 10 chromatographic peaks were extracted as the common peaks of Lavandula Angustifolia, and 2 peaks were identified. The similarity degrees of the 20 batches of Lavandula Angustifolia were above 0.9. All the batches of Lavandula Angustifolia were classified into 3 categories. Conclusion The method is simple and reproducible, and can be used for the standardization and quality control of Lavandula Angustifolia.
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This paper reports on the chemical composition, the in vitro antischistosomal effects, and the cytotoxicity of the essential oil from the leaves of Lavandula angustifolia Mill., Lamiaceae, grown in the Southeastern Brazil. Borneol (22.4%), epi-α-muurolol (13.4%), α-bisabolol (13.1%), precocene I (13.0%), and eucalyptol (7.9%) were the major essential oil constituents. Incubation with essential oil at 200 μg/ml killed all the adult S. mansoni worms after 24 h (LC50 117.7 and 103.9 μg/ml at 24 and 120 h of incubation, respectively). At a concentration of 50 μg/ ml, the essential oil significantly decreased the motor activity and reduced the percentage of egg development after 120 h. In addition, the essential oil separated all the coupled S. mansoni worm pairs into individual male and female at 25 and 50 μg/ml within 120 and 24 h, respectively. This oil was cytotoxic to GM07492-A cells at only concentrations higher than 200 µg/ml (IC50 243.7 µg/ml). These data indicate that LA-EO exhibits moderate in vitro activity against adult S. mansoni and exerts remarkable effects on eggs development.
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The chemical composition of essential oils and essential oil yield obtained from Rosemarinus officinalis (family Lamiaceae) and Lavandula angustifolia (family Lamiaceae) were determined in two harvesting times. Their essential oil was determined by hydro-distillation, and analysed by GC/MS. The results showed that harvesting time had significant effects on the oil content and compositions in both plants. The maximum essential oil percentage was obtained in full flowering stage in rosemary. Also and in lavender maximum linalool percentage (19.2%) was obtained in full flowering, and minimum linalool percentage (0.2%) was shown in the other time. Also the concentration of β – pinene (2.1%), δ-3-carene (1.5%), β – phellandrene (6.6%), Camphor(10.6%), Cryptone (0.8%), α- terpineol (2.3%) and Linalool acetate (1.2%) were higher than befor flowering stage. Therefore the harvesting time have a great importance in the production of essential oil and influenced on the quantity and quality of essential oil. As consequence, the best harvesting time in both medicinal plants was obtained in full flowering stage.
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<i>Objective</i>: It has been reported that essential oils used in aromatherapy effect the nervous, immune and endocrine systems. The purpose of this study was to assess the effects of essential oils on the cardiovascular system in healthy human subjects.<br> <i>Methods</i>: Rosemary essential oil, which reportedly has a vasopressor-like effect, and lavender essential oil which reportedly has a depressor-like effect, were administered to sixty subjects divided into rosemary, lavender, or control groups. Blood pressure and heart rate were measured after two-minutes of smelling. Data were compared among the three groups.<br> <i>Result</i>: Heart rate increased in the rosemary group. In addition to blood pressure and heart rate increased in subjects who expressed a negative impression from smelling a specific essential oils.<br> <i>Conclusion</i>: The results suggest that fragrance preference affects the therapeutic effects of aromatherapy.<br>