Klingler’s Fiber Dissection Method- An Assisting Approach Towards Teaching Neuroanatomy

Background and Objective: The Klingler fiber dissection technique is a simple and less complicated method foridentifying the fine structure of the white fiber tracts of brain. In this study, we have used classical fiber dissectiontechnique by Klinger’s to produce white matter specimens which can be used for explaining anatomy of variouswhite matter tracts to students.Materials and Methods: Five brains specimen removed from formalin fixed human cadavers (3 males and 2female) were used in this study. Klinger’s fibers dissection method was used to obtain white fibers specimen.Dissection of the cerebrum was performed using wooden spatulas, fine curved metal spatulas, fine forceps. Thewhite fibers were exposed by peeling brain with help of wooden spatula to expose the fibers. The dissectionmicroscope was used to isolate small structures.Results: Using the classical Klinger’s technique, we were able to obtain a brain specimen depicting organizationof various white fibres such as corona radiata, superior longitudinal bundle, association fibres with fibrespassing in relation to lentiform nucleus. In another specimen, dissection of right cerebral hemisphere medial tolentiform nucleus showed continuity of white projection fibres of corona radiata as internal capsule. Fibres ofcorpus callosum were delineated in two specimens which displayed spatial disposition of its various parts.Conclusion: White matter fiber of brain are very important for understanding of function of the central nervoussystem function. The Klingler’s fiber dissection technique with other study material can successfully serve thepurpose of the teaching of complex brain architecture of white matter. These dissected specimens will be moreattractive to students, than the mere imagination of white fiber tracts during neuroanatomy classes.

Neural Substrate Responsible for Crossed Aphasia

Crossed aphasia (CA) refers to language impairment secondary to right hemisphere lesion. Imaging analysis on the lesion location of CA has not yet been reported in the literature. This study was proposed to analyze the most prevalent lesion site related to CA. Brain MRI of 7 stroke patients satisfying the criteria for CA were used to define Region of interest (ROIs) before overlaying the images to visualize the most overlapped area. Talairach coordinates for the most overlapped areas were converted to corresponding anatomical regions. Anatomical lesions where more than 3 patients' images were overlapped were considered significant. The overlayed ROIs of 7 patients revealed the lentiform nucleus as the most frequently involved area, overlapping in 6 patients. Our study first demonstrates the areas involved in CA by lesion mapping using brain MRI, and lentiform nucleus is the responsible neural substrate for crossed aphasia.

RECONSTRUCTION AND FUSION WITH MRI OF CAUDATE NUCLEUS, LENTIFORM NUCLEUS AND THALAMUS / 解剖学报

Objective To reconstruct caudate nucleus,lentiform nucleus and thalamus three-dimensionally,fuse these neural structures with (patient's) radiological images and provide anatomical data for diagnosis and treatment of neurosurgical diseases. Methods Chinese Virtual Human dataset was segmented using image-transparency method at first and each image was smoothed by eroding and dilating, then fused neural structures with MRI,the neural structures and brain in MRI were reconstructed and displayed using surface rendering and volume rendering lastly. Results The reconstructed neural structures were smooth,natural and realistic.Their shapes and positions was clearly displayed and could be rotated, observed in any direction.On the fused images,the neural structures and tumor could be seen at the same time.Conclusion The reconstructed structures and fused images have great reference value to teaching and learning and diagnosis and treatment of neurosurgical diseases.