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In a continuing search for biological natural products with structure diversity from traditional Chinese herbs, five new sesquineolignans (1-5) were isolated from an ethyl acetate extract of the twigs of Litsea cubeba. Their structures were elucidated based on MS, 1D and 2D NMR spectroscopic data, as well as experimental electronic circular dichroism (ECD) spectra. Compounds 1-5 showed moderate inhibitory effects against LPS-induced NO production in RAW264.7 macrophages, with IC
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Litsea , Macrófagos , Estructura MolecularRESUMEN
Chemical investigation on the constituents of the ethyl acetate soluble extraction of Litsea cubeba has resulted in the isolation and structure elucidation of thirty compounds, including one sesquiterpene(1), four monoterpenes(2-5), two γ-butyrolactone derivatives(6 and 7), seven tyramine derivatives(8-14), fifteen aromatic compounds(15-29), and one pyrone derivative(30) via various chromatographic techniques and spectroscopic data analysis(MS, IR, 1 D and 2 D NMR). Compounds 1-7, 13 and 14 were obtained from the genus Litsea for the first time.
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Acetatos , Litsea , Monoterpenos , SesquiterpenosRESUMEN
Objective:To establish the purification process of citral in volatile oil from Fructus Litseae by molecular distillation . Methods:The twice molecular distillation , GC and area normalization method were used for extracting citral , detecting the contents and describing the efficacy of purification , respectively .The mainly factor concerned was temperature .The conditions were as follows:the system pressure was 3000 Pa, the scraper speed was 300 r· min-1 , the feeding rate was 7.5 ml· min-1 and the distillation tem-perature was 45℃for the first time molecular distillation; the system pressure was 5 Pa, the scraper speed was 300 r· min-1 , the feeding rate was 7.5 ml· min-1 and the distillation temperature was 45℃ for the second time molecular distillation .GC was utilized under the following conditions:the sample injection was 1 μl, the column temperature was programmed from 70℃to 250℃, the split ratio was 1:100 and the flow rate of carrier gas was 1.0 ml· min-1 .Results: The content and yield of citral was up to 95.0％ and 87.5％, respectively.Conclusion:The method of twice molecular distillation in combination with GC to purify and detect citral is es -tablished in the work, which can provide reference for the research on the chemical components of Litsea cubeba(Lour.) Pers.and the preparation of citral .
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Objective To study inhibition of litsea cubeba oil on biofilm initial formation stage of Candida albicans ,in order to provide new sight for prevention and treatment of refractory infections arising from Candida albicans biofilm .Methods Observe the inhibition effect of different concentrations litsea cubeba oil on yeast budding by serum germ tube experiments .Results 2 -5μL/mL litsea cubeba oil could thoroughly inhibit budding growth of Candida albicans ,but haven′t antiseptic effect ,the inhibition rate of 0 .25 μL/mL litsea cubeba oil on Candida albicans germ was 85 .0% .Conclusion 2-5μL/mL litsea cubeba oil could completely inhibit the budding growth of Candida albicans ,but have no antiseptic effect .0 .25 μL/mL litsea cubeba oil could hardly inhibit the growth of buds .
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Objective To study inhibition of litsea cubeba oil on biofilm initial formation stage of Candida albicans ,in order to provide new sight for prevention and treatment of refractory infections arising from Candida albicans biofilm .Methods Observe the inhibition effect of different concentrations litsea cubeba oil on yeast budding by serum germ tube experiments .Results 2 -5μL/mL litsea cubeba oil could thoroughly inhibit budding growth of Candida albicans ,but haven′t antiseptic effect ,the inhibition rate of 0 .25 μL/mL litsea cubeba oil on Candida albicans germ was 85 .0% .Conclusion 2-5μL/mL litsea cubeba oil could completely inhibit the budding growth of Candida albicans ,but have no antiseptic effect .0 .25 μL/mL litsea cubeba oil could hardly inhibit the growth of buds .
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Twenty five known aromatic glycosides (1-25) and three known sesquiterpene glycosides (26-28) have been isolated from the twigs of Litsea cubeba by using various chromatographic techniques. Their structures were identified by spectroscopic data analysis (MS, IR, 1D and 2D NMR) as (7S,8R)-dehydrodiconiferyl alcohol 4,9'-di-O-β-D-glucopyranoside(1),(7S,8R)-5-methoxydihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside(2), (7S,8R)-urolignoside(3), (7R,8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside(4), saposide B(5), lanicepside A(6), matairesinol-4-O-β-D-glucopyranoside (7), tyraxjaponoside B(8), (+)-lyoniresinol-9'-O-β-D-glucopyranoside (9), alaschanisoside A (10), syringin (11), psoralenoside (12), isopsoralenoside (13), scopolin(14), 2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucopyranoside (15), 3-hydroxy-4,5-dimethoxyphenyl-β-D-glucopyranoside (16), 2-(3,4-dihydroxyphenyl)ethyl-β-D-glucopyrnoside (17), 2-(4-dihydroxyphenyl)ethyl-β-D-glucopyranoside (18), (+)-catechin-7-O-β-D-glucopyranoside (19), 3'-O-methylepicatechin-7-O-β-D-glucopyranoside (20), kaempferitrin (21), quercetin-3-O-α-L-rhamnopyranside (22), kaempferol-3-O-β-D-glucopyranoside (23), kaempferol 3-O-β-D-glucopyranosyl(1→2)-O-β-D-galactopyr anoside-7-O-α-L-rhamnopyranoside (24), quercetin 3-O-α-L-rhamnopyranosyl(1→6)-O-β-D-glucopyranosyl(1→3)-O-α-L-rhamnopyranosyl(1→2)-O-β-D-glucopyranoside (25), staphylionoside D(26), vomifoliol 9-O-β-D-glucopyranoside (27), dihydrovomifoliol-O-β-D-glucopyranoside (28). Compounds 1-21 and 24-28 were obtained from this genus for the first time.
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Objective To analyze the effects of Litsea cubeba oil on gene expression profile of Candida albicans by comparing the differential gene expression profile after exposure to Litsea cubeba oil using genome-wide gene expression array.Methods Candida albicans ATCC90028 was exposed to Litsea cubeba oil for 90 min.Then RNA was isolated and gene expression profiles were compared to identify the differential gene expression profile using cDNA microarray analysis.Results A total of 491 geneswerefoundtoberesponsivetoLitseacubebaoil,accountingforabout11% ofthetotalnumberofgenesinCandida albicans (491/4 634),of which 216 genes were up-regulated and 275 down-regulated.These differentially expressed genes included genes encoding the key target enzyme in ergosterol biosynthesis pathway,genes in stress response,DNA replication and repair,molecular transport,and energy metabolism.Conclusions Litsea cubeba oil has significant effect on the expression of about 1 1% genes of Candida albicans genome.We presume that the genes encoding the key target enzyme in ergosterol biosynthesis pathway may contribute to the action of Litseacubeba oil on Candidaalbicans,which is similar to azole antifungal drugs.However,the role of other differentially expressed genes in the action of Litseacubeba oil on Candidaalbicans remains unclear,which deserves further study to characterize their potential association with the antifungal effect of Litsea cubeba oil.
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Materials and methods: Fruits and leaves of Litsea cubeba (Lour.) Pers. were collected in August 2001. The essential oil analysis was carried out by combination of capillary gas chromatography and mas spectrometry. Results: 16 constituents were identified in the fruit oil, and 27 in the leaf oil. The major components of the fruit oil were geranial (46.83%) and neral (38.77%). The monoterpenic hydrocarbon compounds occurred in a very low amount, except l-limonene (2.14%). The other oxygenated compounds as well as the sesquiterpenic compounds were only about 1%. The major constituents of the leaf oil were cis-ocimene (42.84%) and cineole (10.54%). In addition, there were: linalool (2.07%), terpinene-4-ol (3.65%) and alpha-terpineol (3.65%). The monoterpenic hydrocarbon compounds were found at the level of 74.56%.
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Química , Bioquímica , PlantasRESUMEN
Investigatetion of the fruits and leaves oil of Litsea cubeba (Lour.) Pers. wild growing in the moutain areas in Ba Vi district, Ha Tay province. The major components of the fruits oil of Litsea cubeba (Lour.) Pers. is citral, which includes of neral (citral b) and geranial (citral a) with content over 65% equivalent in some Northern provinces. Fruits and leaves oil analysis were carried out by a combination of capilarry GC and MS. The main components of the leaves oil are linalol (over 85%), 1.8-cineol (-50%), sabinen (10-15%) and -terpineol or -terpinyl acetate (10-20%). This plant in Ba Vi has many biochemical diversification, that is also a character of Lauraceae as Long nao, Vu huong.