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@#Objective To investigate the effects of low frequency stimulation (LFS) on proliferation and angiogenesis of neural stem cells (NSC) in cerebral infarction side in rats. Methods The rats with permanent middle cerebral artery occlusion (MCAO) were randomly divided into sham-operation group, control group and LFS group, each group was divided into 7 days and 14 days subgroups with 12 rats in each subgroup. LFS therapy was started 2 days after operation. The degree of nerve function defect was evaluated with Neurological Severity Score (NSS), and the 5-bromodeoxy-dine (BrdU) positive cells in the subventricular zone (SVZ) of cerebral infarction side were detected with immunofluorescence. Stromal cell-derived factor 1(SDF-1) and vascular endothelial growth factor (VEGF) in infarction side were detected with enzyme-linked immunosorbent assay (ELISA). Results The NSS score was lower in the LFS group than in the control group and the sham-operation group 14 days after surgery (P<0.01). The number of BrdU positive cells, the content of SDF-1 and VEGF in the ischemic side were more in the LFS group than in the other groups (P<0.01) after treatment. Conclusion LFS can improve the neurological function in rats with acute cerebral infarction, which may associate with activating SDF-1/CXCR4 axis.
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Objective To study the effect of low frequency stimulation (LFS) in the pedunculopontine tegmental nucleus (PPTg) on gait and movement ability in unilateral lesioned Parkinson's disease(PD) rats.Methods Thirty-two male SD rats were randomly assigned to control group (6-hydroxydopamine treatment,n=8) and experimental group (6-hydroxydopamine treatment combined with electrode implantation,n=24).All subjects were processed with injection of 6-hydroxydopamine in the fight medial forebrain bundle (MFB) and simultaneously the rats in the experimental group were received an electrode implantation into the ipsilateral PPTg.Catwalk gait and open field test data were acquired at 1 week before stereotactic surgery and 3 weeks after operation in the rats of control and experimental groups; the behavioral data of rats in the experimental group were recorded again 4 weeks after operation when they were under ongoing LFS-PPTg.Results Catwalk results showed that the postoperative couplings values (right front→left front,right front→left hind,right hind →left front,right hind →left hind) were significantly increased in all subjects as compared with the preoperative ones (P＜0.05); while couplings value in rats of the experimental group after LFS-PPTg significantly reduced as compared with that before LFS-PPTg (P＜0.05).The postoperative run speed variation statistically increased and postoperative cadence significantly decreased in all rats as compared with the preoperative ones (P＜0.05); the run speed variation significantly decreased and cadence obviously increased in experimental group after LFS-PPTg as compared with those before LFS-PPTg (P＜0.05).Open field test:the moving distance of all subjects decreased significantly after operation; the distance in rats of the experimental group after LFS-PPTg increased significantly as compared with that before LFS-PPTg.Conclusion LFS-PPTg can effectively improve gait function and motor activity of unilateral PD rat models.
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Long-term potentiation(LTP)is a typical model of synaptic plasticity which has great relationship with learning and memory mechanisms:The induction of LTP depends on the activation of the calcium channels and LTPs generated by calcium ions influx through different channels play different roles in memory.This paper reviews the progress of the research on LTPs induced by activations of N-methyl-D-aspanate receptor(NMDAR)channels and voltage-dependent calcium channels(VDCC).Finally,it gives an example of new forms of low frequency stimulation for induction of LTP.
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@#ObjectiveTo investigate the effect of low frequency stimulation on motor function of stroke patients.Methods50 stroke patients were randomly divided into control group and treatment group with 25 cases in each group. Patients of control group received neuromedical and Bobath technical treatment; cases of treatment group received low frequency stimulation besides the above two methods. All patients were assessed respectively with the Fugl-Meyer Assessment and Clinical Nerve Function Impaired Scale at the admission and on 20 days after treatment.ResultsThe motor functions of both groups were improved (P<0.05). But the recovery of treatment group was superior to the control group (P<0.05).ConclusionLow frequency stimulation can promote the stroke patients' motor function recovery.
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We retrospectively compared the treatment effects of electro-acupuncture against <i>in situ</i> acupuncture, in 15 Hunt's Syndrome patients who underwent acupuncture therapy in our institution between August 1996, and June 2004, and who had fulfilled 3 electroneurography (ENoG) criteria with a value of 0% within 90 days after onset, had a paralysis score of <20, and could be followed up for 6 months after onset. Electroacupuncture therapy was performed on the paralysis side of the faces of 8 patients (the electro-acupuncture group), while <i>in situ</i> acupuncture therapy was performed on the remaining 7 (the <i>in situ</i> acupuncture group). No significant differences were observed in the background factors between the two groups. Treatment results were evaluated using the 40-point paralysis score described by Yanagihara, as well as a sequela score, which was a modified method of the sequela assessment developed by Nishimoto and Murata et al. Changes in paralysis scores from first acupuncture therapy, out to 6 months post-onset were compared using repeated ANOVA measures, and Mann-Whitney U tests respectively. Recovery of paralysis scores did not significantly differ between the two groups (p=0.0507), although slightly better recovery was observed in the electroacupuncture group, as compared with the <i>in situ</i> acupuncture group. Additionally, sequelae scores did not significantly differ between the two groups (p=0.51). In recent years, many have been of the opinion that lowfrequency stimulation is contraindicated. In this study, however, the incidence of sequelae did not differ significantly between the electro-acupuncture therapy and the <i>in situ</i> acupuncture therapy groups, although slightly better paralysis recovery was observed in the former.
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Objective To investigate the effect of low frequency electric deep brain stimulation on amygdale kindling in rats.Methods The amygdale kinkling model of rats was established by operation on the brain.The effects of low frequency deep brain electric stimulation used alone or in combination with anti-epilepsy drugs were ob- served in terms of severity of seizure attack reflected by Racine's scale and afterdischarge duration recorded in electro- encephalogram.Results Fifteen minutes of low frequency electric stimulation at 1 Hz and 100 to 350?A effective- ly inhibited amygdale kindling as demonstrated by a significant decrease of afterdischarge duration,and decreased the severity of seizure attack (P