Determinatoin of macromolecule protein in Chinese materia medica injection / 中草药

Objective: To investigate the feasibility of four methods for macromolecule protein determination in Chinese materia medica (CMM) injection. Methods: The sensitivity, specificity, and protein test results of sulfosalicylic acid, Coomassie brilliant blue, Lowry, and size exclusion chromatograph (SEC) methods on five kinds of CMM injections were compared. Results: Sulfosalicylic acid method had low sensitivity, Coomassie brilliant blue and Lowry methods had poor specificities, and SEC method had high sensitivity and good specificity. No protein was detected in five kinds of CMM injection by SEC. Conclusion: SEC is suitable for the determinatoin of macromolecule protein in CMM injection.