RESUMEN
Objective: To detect the effect of over expressed human MIR31HG gene on the proliferation and m grat on of PC9 ce Is, and to clarify the mechan sm of oncogene MIR31 HG Methods: The ful length sequence of ong non coding RNA (LncRNA) MIR31HG was amp fed by RT PGR and cloned nto the pcDNA3 1 ( ) eukaryot c expression vector. The PG9 cells were transfected w th the pcDNA3 1 MIR31HG overexpress on vector and control vector pcDNA3 1. The construction of MIR31HG overexpress on vector was detected by enzymatic digest on identification. The stab e cell 1 nes w th overexpress on of MIR31HG (PC9 pcDNA3 1 MIR31HG∗ stab e transfection group) and control ce 1 1 nes (PC9 pcDNA3 1, empty vector group) were established by G418 drug screen ng. and the express on evel of MIR31 HG gene n stably transfected cell ine was detected by RT PGR CCK 8 method and scratch heal ng assay were used to detect the proliferation act vities and m gration ab 1 ties of PC9 eel s. Results: The agarose gel e ectrophoresis resu ts showed that the spec f c gene fragment of MIR31HG was obta ned by amplif cation successfu ly. The gene fragments of target gene and vector were produced by double enzyme digest on of MIR31HG eukaryotic expression vector. The RT PGR resu ts showed that the MIR31HG RNA expression leve in the cells n stable transfect on group was sign ficantly h gher than that in empty vector group (P< 0 05). The results of CGK 8 test showed that the pro iferat on act vities of the eels in stable transfection group were s gnif cant y higher than those in empty vector group at 24, 36 and 48 h after culture ( P<0 01). The results of scratch healing assay showed that the scratch heal ng rate of ce Is n stable transfect on group was sign ficantly h gher than that in empty vector group at 48 h after culture ( P<0 05). Conclusion: The eukaryotic overexpression vector and the PG9 eel line stably transfected w th human LncRNA MIR31 HG gene are constructed successfully, and M1R31 HG overexpression can promote the pro iferat on and m grat on of ung cancer PG9 cells.
RESUMEN
BACKGROUND: Psoriasis is a complex, chronic inflammatory skin disease with substantial negative effects on patient quality of life. Long non-coding RNAs (lncRNAs) are able to be involved in multitudes of cellular processes in diverse human diseases. This study aimed to investigate the potential involvement of lncRNA MIR31HG in HaCaT keratinocytes proliferation. RESULTS: The study showed that MIR31HG was significantly elevated in the lesional psoriatic skin compared with normal individuals' skin. Knockdown of MIR31HG inhibited HaCaT keratinocytes proliferation. Flow cytometry analysis showed that siRNA-mediated MIR31HG depletion induced cell cycle arrest in the G2/M phase. In addition, MIR31HG expression was found to be dependent on NF-κB activation. CONCLUSIONS: NF-κB activation mediated MIR31HG upregulation plays an important role in the regulation of HaCaT keratinocytes proliferation. It could be a potential diagnostic biomarker and therapeutic target for psoriasis.