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Objective:To explore the potential value of interim 18F-fluorodeoxyglucose (FDG) PET/CT combined with B-cell lymphoma-2 (Bcl-2)/MYC protein dual expression (DE) status in the prognostic stratification for patients with primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL). Methods:Forty-six patients (21 males, 25 females; age 20-83 years) with newly diagnosed PGI-DLBCL from June 2012 to May 2019 in Nanjing Drum Tower Hospital were enrolled in this retrospective study. Immunohistochemistry for Bcl-2 and MYC protein expression was performed. All patients underwent baseline and interim (after 2-4 cycles of cyclophosphamide, doxorubicin, vincristine, prednisone, and rituximab (R-CHOP) regimen) 18F-FDG PET/CT scans for assessment. Interim 18F-FDG PET/CT results were determined based on Deauville 5-point scale (DS) and changing rate of maximum standardized uptake value (ΔSUV max%) in 18F-FDG PET/CT images. Kaplan-Meier survival analysis, Cox proportional hazards regression model (single factor, multiple factors analysis) were used to analyze the prognosis (3-year progression free survival (PFS) and overall survival (OS) rates). Results:Patients were followed up for 6-84 months, and 14 showed disease progression and 9 died. The PFS rate and OS rate were 69.6% and 80.4%, respectively. DE, DS as well as ΔSUV max% were significant predictors of PFS (hazard ratio ( HR) values: 3.280, 5.120, 9.167, all P<0.05); lactate dehydrogenase (LDH), MYC protein expression, DS and ΔSUV max% were significant predictors of OS ( HR values: 4.091, 9.618, 7.697, 11.151, all P<0.05). Multivariate analysis revealed that DS and ΔSUV max% were independent predictors of PFS and OS ( HR values: 4.370-9.244, all P<0.05). In the DS negative (-) group, patients with DE positive (+ ) had lower PFS and OS rates than those with DE- (PFS rate: 50.0% vs 88.9%; OS rate: 66.7% vs 96.3%; χ2 values: 6.050, 4.966, both P<0.05). In ΔSUV max%<90% group, patients with DE+ had lower PFS rate than those with DE- (12.5% vs 68.8%; χ2=6.649, P=0.01). Conclusions:Interim PET/CT analysis using DS and ΔSUV max% is able to predict survival in PGI-DLBCL patients. The combination of DS, ΔSUV max% and DE can risk-stratify PGI-DLBCL patient more effectively.
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Purpose To investigate the expression of c-myc and MDM2 protein in multiple myeloma (MM) and their correlation with clinical stage.Methods Immunohistochemical SP three-step method was used to detect the expression of MDM2 and c-myc protein in 60 cases of MM with different clinical stages and 10 cases of nontumorous bone marrow tissue.Results The positive rate of c-myc protein in MM and nontumorous bone marrow tissue were 71.7％ and 10.0％;the positive rate of MDM2 protein in two tissues were 80.0％ and 20.0％,respectively.The expression difference of two proteins between MM and nontumorous bone marrow tissue was statistically significant (P ＜ 0.05).The expression of c-myc and MDM2 protein was positively correlated with MM clinical ISS stage (P ＜ 0.05),but irrelevant with the age and gender.The expression of c-myc and MDM2 protein in MM was positively correlated (P ＜ 0.05).Conclusion The c-myc and MDM2 protein have a highly expression in MM,and it may be relative to the occurrence of MM and clinical stage.In the MM,c-myc and MDM2 protein may have synergetic functions and promote the development of tumor.
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Objective To explore inhibitory effects of ginsenoside on the proliferation of human thyroid canc-er SW579 cells in vitro and expression of C -myc and Bcl -2 protein.Methods Human thyroid cancer SW579 cells were cultured according to conventional method.The study was divided into the control group and ginsenoside group (20,40,8ug/mL).Inhibitory role of ginsenoside on proliferation of SW579 cells was detected by MTT assay.Western-blot method was used to determine expression levels of C -myc and Bcl -2 protein in different group.Results The inhibition rates of 20,40,80μg/mL ginsenoside to SW579 thyroid carcinoma cell were 22.35%,51.76% and 68.24% respectively.Which meaned ginsenoside had obvious inhibitory effect compared with the control group(P <0.01),and inhibition increased with the increase of solubility(P <0.01).C -myc and Bcl -2 protein were reduced progressively in place with the increase of ginsenoside concentration by Western -Blot analysis (P <0.01 ).Conclusion Gisenoside may play the inhibitory role on proliferation of human thyroid cancer SW579 cells in vitro, and its mechanism may be related with down -regulation of C -myc and Bcl -2 protein.
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PURPOSE: c-myc, bcl-2 and p53 are known to regulate apoptosis. There has been growing interest in analyzing their contribution to the pathogenesis and prognosis in a variety of human cancers. This study was undertaken to investigate the expression of these proteins in pulmonary adenocarcinomas and to determine their relationship with clinicopathologic parameters and survival. MATERIALS AND METHODS: Archival tumor tissues from 61 patients with adenocarcinoma of lung were analyzed by immunohistochemistry for the expression of c-myc, bcl-2 and p53 proteins. Clinical information was obtained through the computerized retrospect database from the tumor registry. RESULTS: Of 61 patients, 32 were men and 29 women with the median age 63 years. 4 had stage I disease, 2 had stage II disease and 55 had stage III disease. The expression of c-myc protein was identified in 13% (8/61) tumors, bcl-2 protein was detected in 1.6% tumors (1/61) nd p53 was detected in 77% (47/71) tumors. The association of the expression of c-myc, bcl-2 and p53 was not detected. The survival time was longer in patients expressing c-myc protein than in patients without the c-myc protein expression (p=.045). Neither bcl-2 nor p53 showed the correlation to clinicopathologic variables. CONCLUSION: Our data suggest the involvement of p53 alteration in the pathogenesis of lung adenocarcinoma. The c-myc expression in some tumors indicates that c-myc alone may not contribute critically to the development and/or the progression of these tumors. It, however, correlated to the survival time, suggesting the c-myc expression as a favorable prognostic factor possibly through the apoptosis pathway.
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Femenino , Humanos , Masculino , Adenocarcinoma , Apoptosis , Inmunohistoquímica , Neoplasias Pulmonares , Pulmón , PronósticoRESUMEN
Objective To investigate the expression of telomerase-associated genes and c-myc protein before and after eradication of Helicobacter pylori(H.pylori) and to elucidate the possible correlation between human telemerase catalytic subunit(hTERT) and human telomerase RNA(hTR) and c-myc protein. Methods Thirty nine H.pylori positive and 21 negative patients were enrolled. The expression of hTR,hTERT and c-myc protein before and after H.pylori eradication were compared. The expression of hTR was determined by in situ RNA hybridization whereas hTERT and c-myc protein were detected by immunohistochemical staining. Results Before treatment,the positive expression of hTR,hTERT and c-myc protein in H.pylori -positive group were significantly higher than those in H.pylori -negative group(51.3% vs. 19.0%,53.8% vs. 23.8% ,53.8% vs. 28.6%,P 0.05). Conclusions H.pylori infection may upregulate the expression of hTERT by inducing the overexpression of c-myc protein,which can cause telomerase activation and gastric carcinogenesis. The expression of telomerase-associated genes and c-myc protein may decrease or disappear after H.pylori eradication,which can lower the risk of gastric carcinogenesis.
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Objective:To study the anti proliferation and inducing apoptosis effects of cytokine induced killer cells CIK cells on MGC 803 gastric cancer cell lines and to probe its underlying mechanism.Methods:To detect the anti proliferation and the cytotoxicity of CIK cells on MGC 803 gastric cancer line by MTT assay.The morphological changes of the apoptosis cell were observed by HE stain, scanning and transmission electron microscope. The positive expression of p53, p16,C myc were determined by immunocytochemistry (ICC).Results:MTT assay showed that the inhibitive rate inhanced obviously with the addition of Effect/Target rate and extension of time ( P
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Objective To study the effect of spider venom from Macrothele raveni on proliferation and cell cycles of human hepatocellular carcinoma cell line SMMC-7721 and the molecular mechanism of the(effect.) Methods Proliferation of SMMC-7721 cells was determined by MTT assay.DNA synthesis of SMMC-7721 cell pre-and post-treatment with spider venom from M.raveni was tested by -TdR(assay.) The induction of apoptosis and the change of cell cycle in SMMC-7721 cells treated with spider(venom) from M.raveni were investigated by Flow Cytometry.The effect of spider venom from M.raveni on expression of c-myc protein in SMMC-7721 cells was studied by Western Blot.Results MTT assay showed that the proliferation of SMMC-7721 cells in vitro was inhibited by spider venom from M.raveni((P
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Objective: To investigate the expression of Skp2,c-Myc and P27kip1 protein,their association with clinicopathological features and the correlation between one and another.Method:Skp2,c-Myc and P27kip1 protein were examined by using immunochistochemical staining method in 20 cases of normal colorectal mucosa,20 cases of colorectal adenomatous polyp and 60 cases of colorectal cancer,and analysed the relationship with clinicopathologic features and between Skp2 with c-Myc and P27kip1 protein.Results:The positive rates of Skp2,c-Myc and P27kip1 protein in colorectal cancer were 71.67%,66.67% and 21.67% respectively.Moreover,the expression levels of Skp2 and P27kip1 protein in colorectal cancer were significantly associated with the pathologic grade,invasion deep,clinical stage and lymph node metastasis,c-Myc associated with the invasion deep,clinical stage and lymph node metastasis,but were independent of age and sex.Besides,in colorectal cancer,the expression levels of Skp2 and P27kip1 protein were inversely correlated,and the expression levels of Skp2 and c-Myc protein were positively correlated.Conclusions:The expression of Skp2,c-Myc and P27kip1 protein are associated with occurrence and development in colorectal cancer through their alteration,which is very important for assessment of malignancy degree and prognosis in colorectal cancer,and detection cancer of their expression provides theoretical basis for targetting therapy of colorectal.