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Objective:To investigate the influence of magnetic Fe3O4 nanoparticles in the expressions of Caveolin-1 and Clathrin Heavy Chain proteins in organ tissues of the rats, and to clarify its mechanism.Methods:Twenty-four Wistar rats were randomly divided into control group, and low,medium and high doses of magnetic Fe3O4 nanoparticle groups by weights.24 h after tail vein injection of different doses of magnetic Fe3O4 nanoparticles, the organ tissues were obtained.Western blotting method was used to detect the expressions of Caveolin-1 and Clathrin Heavy Chain proteins in the main organ tissues of the rats.Real-time fluorescent quantitative PCR was used to measure the expressions of Caveolin-1 and Clathrin Heavy Chain mRNA.Results:Compared with control group,the expressions levels Clathrin Heavy Chain protein and mRNA in liver and spleen tissues of the rats in medium and high doses groups were significantly increased (P0.05).Compared with control group,the Caveolin-1 mRNA expression levels in liver, spleen, and lung tissues of the rats in low,medium and high doses groups were significantly increased (P0.05).Conclusion:Magnetic Fe3O4 nanoparticles could enhance the expressions of Clathrin Heavy Chain in the liver, spleen, and lung tissues of the rats.Endocytosis of Clathrin Heavy Chain protein is one way for magnetic Fe3O4 nanoparticles into the liver, lung, spleen cells of the rats.
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Magnetic iron oxide (Fe3O4) nanoparticles were sythesized using solvothermal reaction and then coated with titanium oxide (TiO2) via sol-gel process of hydrolysis and condensation of tetrabutyl titanate (TBOT). The obtained Fe3O4-TiO2 particles were characterized with transmission electron microscope (TEM) and dynamic light scattering (DLS). The loading and release of doxorubicin (DOX) were evaluated. Methyl thiazolyl tetrazolium (MTT) method was used to study the cytotoxicity and effect of chemo-photodynamic therapy. The obtained Fe3O4-TiO2 particles were uniform and well dispersed. The loading capacity of DOX was 43%. A pH-sensitive release property of Fe3O4-TiO2-DOX was observed. In the cytotoxicity experiment, cytotoxicity was found upon combination of Fe3O4-TiO2-DOX and ultraviolet (UV), while no obvious cytotoxicity was found in the blank Fe3O4-TiO2 particles. In conclusion, the fabricated Fe3O4-TiO2 nanoparticles exhibited a high loading capacity and excellent photodynamic therapeutic effect, suggesting that it may be used as a novel carrier for chemo-photodynamic therapy of cancer.
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OBJECTIVE: To prepare magnetic nanoparticles with good biocompatiblity and evaluate the photothermal effect, cytotoxicity and photothermal abltion of cancer cells in vitro.
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The effect of magnetic Fe3O4 particles on cellulase in the enzymatic hydrolysis of sunflower seed hull was studied in different adding ways and additive amount. In the process of enzymatic hydrolysis of sunflower seed hull, the variations of cellulase activity, reducing sugar concentration and cellulose conversion were evaluated. After the reaction, the analysis of pH and surface tension of hydrolysate were also used to determine the mechanisms of cellulase by the magnetic effect. The results indicated that after adding magnetic Fe3O4, the cellulase activity, reducing sugar concentration and conversion of cellulose had an increased between the 0.5 g/L and 2.0 g/L cases after 48 h. When the additive amount of magnetic Fe3O4 was 2 g/L, the cellulase activity at 60 h was improved significantly by 25.9%. It was found that the concentration of reducing sugar was increased from 6.950 mg/mL to 8.775 mg/mL with magnetic Fe3O4 1.5 g/L. Simultaneously, compared with the blank, which the conversion of cellulose was 47.932%, the maximum celluloseconversion of samples with adding magnetic Fe3O4 was 60.531%. Besides, the stability of cellulase activity adding in times was better than in one time. After the reaction, the final surface tension of hydrolysate with 1.5 g/L magnetic Fe3O4 was the lowest in comparison with the blank. However, no significant differences were observed in the final pH of the hydrolysate.