RESUMEN
OBJECTIVE:To pre pare Glycyrrhetinic acid-modified docetaxel magnetic nanoparticles (GA-DTX-NGO/IONP- NPs),and to evaluate its physicochemical properties. METHODS :Magnetic nano graphene oxide (NGO/IONP)was chosen as the anti-tumor drug carrier ,docetaxel(DTX)as the model drug and glycyrrhetinic acid (GA)as the target molecule. Firstly ,NGO/ IONP was synthesized by hydrothermal method and GA-CS was synthesized by amidation reaction. Fourier IR spectrometer ,DSC and vibration sample magnetic measuring instrument were used to characterize NGO/IONP and GA-CS. GA-DTX-NGO/IONP-NPs Huperzine A in the nicotinic acetylcholine receptor alleviates Aβ -induced 1-42 treatment of Alzheimer ’s disease and vascular dementia :a neurotoxicity via downregulation of p 38 and JNK MAPK meta-analysis[J]. Evid Based Complement Alternat Med , signaling pathways[J]. Neurochem Int ,2018. DOI :10. 2014. DOI :10.1155/2014/363985. 1016/j.neuint.2018.09.005. were prepared by the ion gelation method. TEM and particle size analyzer were used to observe and determine the morphology , particle size and Zeta potential of GA-DTX-NGO/IONP-NPs ;the ultrafiltration-centrifugation method was used to determine encapsulation efficiency and drug loading amount ;the magnetic properties were investigated by investigating the state with or without external magnetic field ;the photothermal conversion test was carried out with laser irradiation of 808 nm. RESULTS :NGO/ IONP and GA-CS were successfully synthesized ,and NGO/IONP exhibited superparamagnetism characteristics. GA-DTX-NGO/ IONP-NPs were spherical under TEM ,the particle size was (262.8±4.23)nm and the Zeta potential was (13.6±1.51)mV. The encapsulation rate and drug loading amount were (94.29±0.50)% and(17.12±0.12)%,respectively. GA-DTX-NGO/IONP-NPs were black in appearance and evenly dispersed. Under the external magnetic field ,the magnetic nanoparticles could move directionally,showing good magnetic properties. GA-DTX-NGO/IONP-NPs showed a good concentration- and time-dependent photothermal conversion effect under 808 nm laser irradiation. CONCLUSIONS :GA-DTX-NGO/IONP-NPs are successfully prepared. This study could provide some theoretical basis for the combined treatment of magnetic heating-chemotherapy for liver tumors.