Determination of strychnine and brucine in maqianzi san by RP-HPLC / 中国药学杂志

OBJECTIVE: To develop a HPLC method for assaying strychnine and brucine in traditional Chinese drug Maqianzi San. METHODS: With μBondapak C18 (10 μm) column (4 mm×300 mm), MeOH-H2O-HAc-(C2H5)3N(70∶230∶2.4∶0.3) as mobile phase, we set a detector at 254 nm. RESULTS: Linear range of strychnine and brucine was 0.367 2 μg～0.061 2 μg and 0.945 0 μg～0.157 5 μg respectively. The recovery rate of strychnine and brucine was 100.36% (RSD=1.09%) and 100.01% (RSD=1.46%) respectively. CONCLUSION: This method provides a simple and feasible way to control the quality of Maqianzi San.