Effect of microRNA-138 regulation of Wnt pathway on the biological behavior of human glioma cells in vitro / 解剖学报

Objective To investigate the effect of microRNA (miR)-138 regulation of Wnt signaling pathway on the biological behavior of human glioma cells in vitro. Methods Glioma cell lines U-87MG and U251 were selected and randomly divided into blank group, miR-NC group, miR-138 mimics group and miR-138 inhibitor group. Real-time PCR was used to detect the miR-138 expression in each group; MTT, flow cytometry, Transwell assay and scratch assay were used to detect proliferation, apoptosis, invasion and migration ability of each group respectively, and Western blotting was used to detect Wnt pathway-related protein expression in each group. Results The miR-138 expression level was higher in the miR-138 mimics group compared with the remaining 3 groups, and that in the miR-138 inhibitor group was lower than that in the blank group and the miR-NC group (P<0. 05) ; Compared with the blank group, the cell proliferation rate was lower in the miR-138 mimics group and higher in the miR-138 inhibitor group, and was time-dependent (P<0. 05) ; The apoptosis rate in the miR-138 mimics group was higher than that in the blank group, miR-NC group, and miR-138 inhibitor group, while the apoptosis rate in the miR-138 inhibitor group was lower than that in the rest other groups (P<0. 05) ; The number of cell-invading cells in the miR-138 mimics group was lower than that in the blank group, miR-NC group, and miR-138 inhibitor group, while all miR-138 inhibitor group were higher than the remaining three groups (P<0. 05) ; The cell migration rate of miR-138 mimics group was lower than that of blank group, miR-NC group and miR-138 inhibitor group, while all miR-138 inhibitor group were higher than the remaining three groups (P<0. 05) ; Wnt3a, Wntl, glycogen synthase kinase 3(3(GSK-3(3) and (3-catenin protein expression in the miR-138 mimics group was lower than that in the blank group, miR-NC group, and miR-138 inhibitor group; While miR-138 inhibitor groups were higher than the remaining three groups(P<0. 05). Conclusion MiR-138 overexpression effectively inhibite the proliferation, invasion and migration of glioma cells and promote their apoptosis, probably achieved by pathway inhibition of the Wnt signaling pathway.

Regulatory effects of miR-138 and RUNX3 on Th1/Th2 balance in peripheral blood of children with cough variant asthma / 中国当代儿科杂志

OBJECTIVES@#To study the expression levels of microRNA-138 (miR-138) and Runt-related transcription factor 3 (RUNX3) in peripheral blood of children with cough variant asthma (CVA) and their regulatory effects on Th1/Th2 balance.@*METHODS@#Sixty-five children with CVA (CVA group) and 30 healthy children (control group) were enrolled. Peripheral venous blood samples were collected for both groups, and CD4@*RESULTS@#Compared with the control group, the CVA group showed significantly decreased levels of IFN-γ and IL-2 from CD4@*CONCLUSIONS@#MiR-138 regulates Th1/Th2 balance by targeting RUNX3 in children with CVA, providing a new direction for the treatment of CVA.

Effects of miR-138 on invasion of brain glioma cells through targeting Sema4C / 国际肿瘤学杂志

Objective To explore the effects and mechanism of microRNA-138 (miR-138) on brain glioma cells invasion and migration.Methods The expression of miR-138 was detected by real time quantitative polymcrase chain reaction (qRT-PCR) in 60 cases of glioma tissues and para-carcinoma tissues,and the relationship between miR-138 expression and glioma grading was analyzed.Human glioma cells line U87 and U251 were transfected with miR-138 mimic and negative control (miR-NC).The expression of miR-138 was detected by qRT-PCR.The migration and invasion abilities were tested by Transwell assay,and the expression of semaphoring 4C (Sema4C) protein was tested by Western blotting.Results The expression level of mniR-138 in glioma tissues (2.46 ± 1.07) was significantly lower than that in normal brain tissues (4.83 ±1.16,t =-11.631,P ＜0.001),and miR-138 expression was negatively correlated with tumor grade (r =-O.563,P =0.001).The expression level of miR-138 in cells was significantly higher after being transfected with miR-138 mimic (U251:3.96 ±0.16;U87:4.43 ±0.96) than miR-NC (U251:2.32 ±0.36;U87:2.58± 0.62,t =7.253,P ＜ 0.001;t =8.872,P ＜ 0.001).The ability of invasion and migration were lower after being transfected with miR-138 mimic (U251:89±9;U87:95 ± 10) than miR-NC (U251:206 ± 15;U87:240 ± 20,t =36.629,P ＜ 0.001;t =35.521,P ＜ 0.001).The expression of Sema4C protein was lower after being transfected with miR-138 mimic (U251:0.41 ± 0.06;U87:0.36-± 0.03) than miR-NC (U251:1.01±0.08;U87:1.03±0.13,t=-32.862,P＜0.001;t=-27.512,P＜0.001).Conclusion The up-regulated expression of miR-138 can suppress glioma cells migration and invasion,which might be related to the negative regulation expression of Sema4C protein.

Mechanism of microRNA-138-5p inhibiting proliferation, migration and invasion of lung cancer cells / 中国病理生理杂志

AIM:To investigate the mechanism of microRNA-138-5p (miR-138-5p) inhibiting the proliferation,migration and invasion abilities of lung cancer cells.METHODS:The lung cancer A549 and H460 cells were transfected with miR-NC (control group) or miR-138-5p (experimental group).The bioinformatic analysis was performed to predict the target genes of miR-138-5p.The expression levels of miR-138-5p,forkhead box protein C1 (FOXC1) mRNA and vimentin mRNA were detected by RT-qPCR.The protein expression of FOXC1,vimentin,E-cadhcrin,N-cadherin and β-catenin was determined by Western blot.MTS method and colony formation assay were used to detect cell viability and proliferation ability.Wound healing assay and Transwell assay were used to detect cell migration and invasion ability.RESULTS:Over-expression of miR-138-5p significantly reduced the expression of FOXC1 and vimentin at mRNA and protein levels (P ＜ 0.05).The expression of E-cadherin and β-catenin were up-regulated and the expression of N-cadherin was down-regulated.The proliferation,migration and invasion abilities of the lung cancer cells were inhibited by the over-expression of miR-138-5p.CONCLUSION:miR-138-5p inhibits the proliferation,migration and invasion abilities of lung cancer cells by targeting FOXC1 and vimentin.It may be a potential target for lung cancer gene therapy.

Mechanism of microRNA-138-5p inhibiting proliferation, migration and invasion of lung cancer cells / 中国病理生理杂志

AIM:To investigate the mechanism of microRNA-138-5p (miR-138-5p) inhibiting the proliferation,migration and invasion abilities of lung cancer cells.METHODS:The lung cancer A549 and H460 cells were transfected with miR-NC (control group) or miR-138-5p (experimental group).The bioinformatic analysis was performed to predict the target genes of miR-138-5p.The expression levels of miR-138-5p,forkhead box protein C1 (FOXC1) mRNA and vimentin mRNA were detected by RT-qPCR.The protein expression of FOXC1,vimentin,E-cadhcrin,N-cadherin and β-catenin was determined by Western blot.MTS method and colony formation assay were used to detect cell viability and proliferation ability.Wound healing assay and Transwell assay were used to detect cell migration and invasion ability.RESULTS:Over-expression of miR-138-5p significantly reduced the expression of FOXC1 and vimentin at mRNA and protein levels (P ＜ 0.05).The expression of E-cadherin and β-catenin were up-regulated and the expression of N-cadherin was down-regulated.The proliferation,migration and invasion abilities of the lung cancer cells were inhibited by the over-expression of miR-138-5p.CONCLUSION:miR-138-5p inhibits the proliferation,migration and invasion abilities of lung cancer cells by targeting FOXC1 and vimentin.It may be a potential target for lung cancer gene therapy.