RESUMEN
Objective To establish a quality standard and in vivo efficacy system for Mingmu Guben granule. Methods Chrysanthemi flos and Lycii fructus were identified by thin layer chromatography(TLC)method. The content of chlorogenic acid in Mingmu Guben granule was determined by HPLC. The Welchrom C18(200 mm×4.6 mm,5μm)column was used. The mobile phase consisted of acetonitrile-0.4%phosphoric acid of water(10∶90,V/V)at a flow rate of 1.0 ml/min. The detection wavelength was at 327 nm under 25℃. The in vivo efficacy of Mingnu Guben granule was measured by cataract,retinal ischemia and renal dysfunction rat model. Results The spots in the were clear. The chlorogenic acid showed a good linear relationship within the range of 2.5-90μg/min(r=0.9998). The average recovery was 96.93%,and the RSD was 2.65%. Moreover,the in vivo efficacy of Mingnu Guben granule was measured. The results indicated that orally taking Mingnu Guben granule prevent rat from cataract,retinal ischemia and renal dysfunc?tion induced by adenine. Conclusion By establishing simple,accurate and repeatable methods and measuring in vivo efficacy,our present work provides a quality control system for Mingmu Guben granule.
RESUMEN
Objective To establish a quality standard and in vivo efficacy system for Mingmu Guben granule. Methods Chrysanthemi flos and Lycii fructus were identified by thin layer chromatography(TLC) method. The content of chlorogenic acid in Mingmu Guben granule was determined by HPLC. The Welchrom C18(200 mm×4.6 mm,5 µm) column was used. The mobile phase consisted of acetonitrile -0.4% phosphoric acid of water(10:90, V/V) at a flow rate of 1.0 ml/min. The detection wavelength was at 327 nm under 25°C. The in vivo efficacy of Mingnu Guben granule was measured by cataract, retinal ischemia and renal dysfunction rat model. Results The spots in the were clear. The chlorogenic acid showed a good linear relationship within the range of 2.5-90 µg/min (r=0.9998). The average recovery was 96.93%, and the RSD was 2.65%. Moreover, the in vivo efficacy of Mingnu Guben granule was measured. The results indicated that orally taking Mingnu Guben granule prevent rat from cataract, retinal ischemia and renal dysfunction induced by adenine. Conclusion By establishing simple, accurate and repeatable methods and measuring in vivo efficacy, our present work provides a quality control system for Mingmu Guben granule.