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Objective To investigate the relationship between the methylation status of mucoprotein 3A (MUC3A)gene and the prognosis of children with neuroblastoma(NB).Methods A pathological section of 92 cases of NB from January 2013 to December 2015 in Department of Pathology,Children and Women′s Healthcare Hospital of Laiwu City,were co-llected.There were 43 males and 49 females;and their ages ranged from 3 months to 13 years old, in which 31 cases were less than 1 year old,and 61 cases were over 1 year old.Methylation specific polymerase chain reaction was used to detect the methylation status of MUC3A gene in 92 cases of NB,and the relationship between the methylation status of MUC3A gene and the clinicopathological features of NB were analyzed.According to the methyla-tion status of MUC3A gene,the 3-year survival rate and survival time of the MUC3A gene methylation positive group and the negative group were compared.Results For those over 1 year old,the pathological types were differentiated +undifferentiated and in clinical stage of stage Ⅲ+Ⅳ,their MYCN gene amplification,MUC3A gene methylation inci-dence(86.89%,91.80%,90.32%,81.82%)were significantly higher than those less than 1 year old,whose patho-logical types were well differentiated and in clinical stage of Ⅰ + Ⅱ,MYCN gene was not amplified(38. 71%, 29.03%,30. 00%,42.31%),and the differences were statistically significant(χ2= 23.007,39. 059,35. 480, 14.043,all P<0.001).The 3 year survival rate of MUC3A gene methylation positive children(26.15%)was signifi-cantly lower than that of MUC3A gene methylation negative children(66.67%),and the difference was statistically sig-nificant(χ2=13.283,P<0.001).The median survival time of MUC3A gene methylation positive children in 3-year old children was 19 months,significantly shorter than that of MUC3A gene methylation negative children(32 months), and the difference was statistically significant(log-rank:χ2=5.910,P=0.015).Conclusion The positive methyla-tion of MUC3A gene suggests that children with NB have poor prognosis.
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Akkermansia muciniphila planted at the end of ileum and on the outer layer of intestinal grume for colon and cecum is a kind of dominant bacteria using mucoproteins as the culture medium. On the one hand, it can provide energy to degrade mucoprotein for protection of epithelial cell of intestinal mucosa and decline the permeability of mucosal barrier. On the other hand, it can preferentially use the mucoprotein. As known to all, excessive abundance will weaken the mucosal barrier and the occurrence and development of type 2 diabetes is closely related to the long-term chronic low-intensity inflammatory reaction arising from the damage of intestinal barrier function. Therefore, to improve the intestinal flora and protect intestinal mucosal barrier, A. muciniphila has served as a new idea to treat type 2 diabetes. As A. muciniphila is the dominant bacteria on the outer layer of intestinal mucosa closely relevant to the intestinal mucosal barrier function, this article has reviewed its physiological property and its role in curing type 2 diabetes.
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Objective To approach the effects of decoction changed from Didangtang and Xiaoxianxiongtang Modified Formula on mucoprotein in stratum medium and the III-type collagen level of serum in experimental rats with fibrosis disease in lung interstitial tissue. Methods 40 male Wistar rats were randomly divided into 4 groups, that is pseudo-operation group, model control group, traditional Chinese drug group (TCD) and western medicine group (WM). Every group has 10 rats. Acid hydroc bleocin was dripped disposably into trachea of every group rats except pseudo-operation group, and Sodium Chloride with same volume as Acid hydroc bleocin was dripped disposably into trachea of pseudo-operation group. 24 hours after operation, the rats of TCD was lavaged with Decoction changed from Didangtang and Xiaoxianxiongtang Modified Formula, and the rats of WM was lavaged with Prednisone physiological saline solution. The rats of pseudo-operation group and model control group was lavaged with Sodium Chloride of same volume as TCD and WM. 28 days after operation, the rats was executed. And contents of mucoprotein in stratum medium of and the III-type collagen serum was detected. Results Didangtang and Xiaoxianxiongtang Modified Formula could decrease contents of mucoprotein in stratum medium of and the III-type collagen in serum of experimental rats, and extenuate fibro-accrementition in lung interstitial tissue. Conclusion Didangtang and Xiaoxianxiongtang Modified Formula has the function of treating fibrosis disease in lung interstitial tissue probably by decreasing contents of extracellular matrix in experimental rats with fibrosis disease in lung interstitial tissue.