RESUMEN
The mycelial biomass production of four strains (WS218-1, WS218-2, CST01, and CST02) of Pleurotuscystidiosus in liquid culture using coconut water as a medium was evaluated. Mycelia were extracted and the1,1-diphenyl-2-picrylhydrazyl radical scavenging activities and total phenolic contents were analyzed. Themycochemicals of the best strain were also screened using a thin-layer chromatography spot test. Resultsrevealed that WS218-2 strain produced the heaviest mycelial biomass (6.57 g fresh wt. and 0.39 g dry wt.)while CSC02 and WS218-1 strains registered the lowest fresh and dry weight, respectively. Ethanolic extractof the WS218-2 strain showed the highest radical scavenging activity (79.01%) and contained the highestphenolic content (95.67 mg gallic acid equivalents/g of sample). Eleven mycochemicals including essential oil,triterpenes, anthraquinones, tannins, flavonoids, phenols, fatty acids, alkaloids, steroids, sugars, and coumarinswere detected in WS218-2 mycelia. Pleurotus cystidiosus mycelia could be a valuable source of naturalantioxidants and bioactive metabolites and it is strain-dependent.
RESUMEN
The present study aimed at the evaluation of chemical composition and bioactive potential of methanolic extract obtained from Lepista sordida in terms of antioxidative and antimicrobial efficacy. The macrofungus is recognized for its high nutritional value and medicinal properties. However, to the best of our knowledge bioactivity of its methanolic extract is yet to be explored. In this investigation, quantitative analysis of mycochemicals revealed the extract contained significant amount of phenolic compounds such as phenols and flavonoids. Ascorbic acid was found in higher amount than β carotene and lycopene which were present in vestigial amounts. A phenolic profile was also determined using high performance liquid chromatography that further confirmed the presence of 10 phenolic constituents in the extract. Furthermore, the extract was subjected for determining antioxidant potential in different in-vitro assays. The findings showed remarkable 2, 2-Diphenyl-1-picrylhydrazyl and ABTS radical scavenging ability which was evident by low EC50 values, 330 µg/mL and 30 µg/mL respectively. The extract also demonstrated good chelating and reducing ability, an important marker of antioxidant compounds. Antimicrobial screening displayed positive results against Staphylococcus aureus and Escherichia coli. Altogether, the observations recommend therapeutic application of this mycotaxon on a commercial basis.